IGVFDS6319CCJO

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

MYC

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Construct Library Sets

IGVFDS7857SYCK guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR1_Rep1_S137

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR1_Rep2_S192

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR2_Rep1_S143

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR2_Rep2_S198

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR3_Rep1_S149

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR3_Rep2_S204

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR4_Rep1_S155

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR4_Rep2_S210

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM8399ZECZ

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS4580MCDT

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI7956SVSZ, IGVFFI7037YPDM, IGVFFI1162VBEH, IGVFFI1601HIKR, IGVFFI1646OHQB, IGVFFI4444YPRQ, IGVFFI1192EKYN, IGVFFI5750HKEP

jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR1_Rep1_S137
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR1_Rep2_S192
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR2_Rep1_S143
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR2_Rep2_S198
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR3_Rep1_S149
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR3_Rep2_S204
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR4_Rep1_S155
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinC-PCR4_Rep2_S210

Jesse Engreitz, Stanford

IGVFDS5394TNHK

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI9629PGSJ