IGVFDS6275PORW

released

File Set Type

experimental data

Summary

CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3

Assay Term

in vitro CRISPR screen using flow cytometry

Sample Summaries

TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin C for 51-75% expression of NOS3) modified with a guide library

Construct Library Sets

IGVFDS8953DFJK Guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3

Protocols

https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1

Aliases

jesse-engreitz:measurement_NOS3_CRISPRi_FF_R2_FFrep1_binC_PCR1_S131

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM3812UYWH

InVitroSystem

TeloHAEC

TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin C for 51-75% expression of NOS3) modified with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO3459DFTW

igvf:donor_of_teloHAEC

female

Homo sapiens

Auxiliary Datasets

1 item

Accession

Type

Summary

Files

Aliases

Lab

IGVFDS0715PLIK

AuxiliarySet

gRNA sequencing for CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3

IGVFFI8197FBZG, IGVFFI2545MKMB, IGVFFI4447FSGS

jesse-engreitz:auxiliary_NOS3_CRISPRi_FF_R2_FFrep1_binC_PCR1_S131

Jesse Engreitz, Stanford

Documents

1 item

Page

Description

Type

Download

Preview

experimental overview

experimental protocol

NOS3_VFF_design.pdf

Attribution

Award

HG011972

Principal Investigator(s)

Anshul Kundaje, Jesse Engreitz, Marlene Rabinovitch, Lars Steinmetz, Michael Bassik, Thomas Quertermous, Will Greenleaf

Contact P.I.

Jesse Engreitz

Lab

Jesse Engreitz, Stanford