IGVFDS6262JHMS

released

File Set Type

experimental data

Control Type

low FACS signal

Summary

low FACS signal CRISPR activation FACS screen sorted on the expression of CCR7 integrating a guide (sgRNA) library targeting transcription start sites in many genes

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

CCR7

Sample Summaries

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Protocols

Aliases

charles-gersbach:cd8-tcells-measurement-set-crispra-ccr7-low-bin-rep3

Publications

Transcriptional and epigenetic regulators of human CD8+ T cell function identified through orthogonal CRISPR screens

Sequencing Results (Illumina)

1 item

Accession

File Format

Content Type

Illumina Read Type

Sequencing Run

Read Names

Associated seqspec Files

Sequencing Platform

Flowcell ID

Lane

Index

File Size

Lab

Upload Status

IGVFFI4454GSFQ

fastq

reads

Illumina MiSeq

000000000-K4RT4

54 MB

Charles Gersbach, Duke

validated

Samples

1 item

Accession

Type

Sample Terms

Associated Construct Library Sets

Summary

IGVFSM4110AXZO

PrimaryCell

CD8-positive, alpha-beta memory T cell

IGVFDS4163YHBI

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)