IGVFDS6224TNVO

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

MYC

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Construct Library Sets

IGVFDS7857SYCK guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR1_Rep1_S30

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR1_Rep2_S85

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR2_Rep1_S36

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR2_Rep2_S91

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR3_Rep1_S42

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR3_Rep2_S97

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR4_Rep1_S48

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR4_Rep2_S103

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9724UBWS

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS6296UIVP

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI6708LGQK, IGVFFI8897HGJW, IGVFFI0849URVD, IGVFFI4236ZKZQ, IGVFFI4472ZCED, IGVFFI5078FJXF, IGVFFI8775RJJR, IGVFFI8620KXPV

jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR1_Rep1_S30
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR1_Rep2_S85
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR2_Rep1_S36
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR2_Rep2_S91
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR3_Rep1_S42
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR3_Rep2_S97
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR4_Rep1_S48
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR4_Rep2_S103

Jesse Engreitz, Stanford

IGVFDS8214HXSU

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI3328SYVP