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IGVFDS6156TPWX

released
File Set Type
experimental data
Summary
CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
Assay Term
in vitro CRISPR screen using flow cytometry
Targeted Genes
Sample Summaries
  • Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
Construct Library Sets
  • IGVFDS8183QSJO guide (sgRNA) library targeting transcription start sites in IL2RA
Protocols
Aliases
charles-gersbach:jurkats-measurement-set-crispra-vp64-il2ra-high-bin-rep1
Publications

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Donors

1 item
Accession
Aliases
Sex
Taxa
  • charles-gersbach:jurkat-tcells-donor-1
unspecified
Homo sapiens

Control File Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
  • charles-gersbach:jurkats-measurement-set-crispra-vp64-il2ra-low-bin-rep1
Charles Gersbach, Duke

Auxiliary Datasets

1 item
Accession
Type
Summary
Files
Aliases
Lab
AuxiliarySet
gRNA sequencing for CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
  • charles-gersbach:jurkats-auxiliary-set-crispra-vp64-il2ra-high-bin-rep1
Charles Gersbach, Duke

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke