{
"@context": "/terms/",
"@id": "/measurement-sets/IGVFDS5918RSZR/",
"@type": [
"MeasurementSet",
"FileSet",
"Item"
],
"accession": "IGVFDS5918RSZR",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep2-FFrep2-BinE_AmpliconSequencing"
],
"assay_term": {
"@id": "/assay-terms/OBI_0003661/",
"status": "released",
"term_name": "in vitro CRISPR screen using flow cytometry"
},
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Released in vitro system [IGVFSM5988GKVB](/in-vitro-systems/IGVFSM5988GKVB/) has in progress subobject construct library set [IGVFDS6377KNCH](/construct-library-sets/IGVFDS6377KNCH/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/in-vitro-systems/IGVFSM5988GKVB/"
},
{
"category": "missing sequencing kit",
"detail": "Measurement set [IGVFDS5918RSZR](/measurement-sets/IGVFDS5918RSZR/) has sequence file(s) [IGVFFI7145EKZE](/sequence-files/IGVFFI7145EKZE/), [IGVFFI8588AULD](/sequence-files/IGVFFI8588AULD/), [IGVFFI8706LWAP](/sequence-files/IGVFFI8706LWAP/), [IGVFFI9124AKYT](/sequence-files/IGVFFI9124AKYT/), [IGVFFI6657ZPZS](/sequence-files/IGVFFI6657ZPZS/), [IGVFFI5114CJUG](/sequence-files/IGVFFI5114CJUG/), [IGVFFI4247LKDN](/sequence-files/IGVFFI4247LKDN/), [IGVFFI7161KOGZ](/sequence-files/IGVFFI7161KOGZ/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_inconsistent_sequencing_kit",
"path": "/measurement-sets/IGVFDS5918RSZR/"
},
{
"category": "missing sequence specification",
"detail": "Measurement set [IGVFDS5918RSZR](/measurement-sets/IGVFDS5918RSZR/) has sequence file(s): [IGVFFI7145EKZE](/sequence-files/IGVFFI7145EKZE/), [IGVFFI8588AULD](/sequence-files/IGVFFI8588AULD/), [IGVFFI8706LWAP](/sequence-files/IGVFFI8706LWAP/), [IGVFFI9124AKYT](/sequence-files/IGVFFI9124AKYT/), [IGVFFI6657ZPZS](/sequence-files/IGVFFI6657ZPZS/), [IGVFFI5114CJUG](/sequence-files/IGVFFI5114CJUG/), [IGVFFI4247LKDN](/sequence-files/IGVFFI4247LKDN/), [IGVFFI7161KOGZ](/sequence-files/IGVFFI7161KOGZ/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_missing_seqspec",
"path": "/measurement-sets/IGVFDS5918RSZR/"
}
],
"WARNING": [
{
"category": "missing auxiliary set",
"detail": "Measurement set [IGVFDS5918RSZR](/measurement-sets/IGVFDS5918RSZR/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
"level": 40,
"level_name": "WARNING",
"name": "audit_missing_auxiliary_set",
"path": "/measurement-sets/IGVFDS5918RSZR/"
}
]
},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
"title": "Jesse Engreitz"
},
"title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
},
"control_file_sets": [
{
"@id": "/measurement-sets/IGVFDS9477ARWI/",
"accession": "IGVFDS9477ARWI",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-Jurkat-PE2_Exp1_NegControl_AmpliconSequencing",
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-Jurkat-PE2_Exp1_NegControl_AmpliconSequencing"
],
"status": "released"
}
],
"control_for": [],
"creation_timestamp": "2023-11-30T22:48:14.969371+00:00",
"donors": [
{
"@id": "/human-donors/IGVFDO1080XFGV/",
"accession": "IGVFDO1080XFGV",
"aliases": [
"igvf:donor_of_Jurkat"
],
"sex": "male",
"status": "released",
"taxa": "Homo sapiens"
}
],
"externally_hosted": false,
"file_set_type": "experimental data",
"files": [
{
"@id": "/sequence-files/IGVFFI7145EKZE/",
"accession": "IGVFFI7145EKZE",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep2-FFrep2-BinE-PCRrep1_R1"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T19:34:29.424303+00:00",
"file_format": "fastq",
"file_size": 7495280,
"href": "/sequence-files/IGVFFI7145EKZE/@@download/IGVFFI7145EKZE.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/4e4c026a-f972-48db-a2aa-cb0489b8e83e/IGVFFI7145EKZE.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S16-BioRep2-FFRep2-BinE-PCRrep1_S138_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI8588AULD/",
"accession": "IGVFFI8588AULD",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep2-FFrep2-BinE-PCRrep2_R2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T20:16:04.877843+00:00",
"file_format": "fastq",
"file_size": 14698884,
"href": "/sequence-files/IGVFFI8588AULD/@@download/IGVFFI8588AULD.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/681dba56-2040-4e72-96e8-631cbd5d49c5/IGVFFI8588AULD.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S16-BioRep2-FFRep2-BinE-PCRrep2_S195_R2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI8706LWAP/",
"accession": "IGVFFI8706LWAP",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep2-FFrep2-BinE-PCRrep2_R1"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T20:07:40.842601+00:00",
"file_format": "fastq",
"file_size": 7813467,
"href": "/sequence-files/IGVFFI8706LWAP/@@download/IGVFFI8706LWAP.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/fe264e20-ba27-40e5-bb1a-8006eb470e5d/IGVFFI8706LWAP.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S16-BioRep2-FFRep2-BinE-PCRrep2_S195_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI9124AKYT/",
"accession": "IGVFFI9124AKYT",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep2-FFrep2-BinE-PCRrep1_I1"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T19:51:20.945035+00:00",
"file_format": "fastq",
"file_size": 3283555,
"href": "/sequence-files/IGVFFI9124AKYT/@@download/IGVFFI9124AKYT.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/f69fcfbb-ccd5-4707-b39c-02afd0c47c46/IGVFFI9124AKYT.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S16-BioRep2-FFRep2-BinE-PCRrep1_S138_I1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI6657ZPZS/",
"accession": "IGVFFI6657ZPZS",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep2-FFrep2-BinE-PCRrep1_R2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T19:42:53.427105+00:00",
"file_format": "fastq",
"file_size": 13903734,
"href": "/sequence-files/IGVFFI6657ZPZS/@@download/IGVFFI6657ZPZS.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/5a0f426b-a321-4bd1-a6f8-6febea595d10/IGVFFI6657ZPZS.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S16-BioRep2-FFRep2-BinE-PCRrep1_S138_R2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI5114CJUG/",
"accession": "IGVFFI5114CJUG",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep2-FFrep2-BinE-PCRrep2_I1"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T20:24:33.407033+00:00",
"file_format": "fastq",
"file_size": 3365153,
"href": "/sequence-files/IGVFFI5114CJUG/@@download/IGVFFI5114CJUG.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/469e015d-62cb-4616-a7de-d4eb6e6b0a20/IGVFFI5114CJUG.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S16-BioRep2-FFRep2-BinE-PCRrep2_S195_I1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI4247LKDN/",
"accession": "IGVFFI4247LKDN",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep2-FFrep2-BinE-PCRrep1_I2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T19:59:27.995965+00:00",
"file_format": "fastq",
"file_size": 3137604,
"href": "/sequence-files/IGVFFI4247LKDN/@@download/IGVFFI4247LKDN.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/65531476-f2f5-4ca5-9228-6e2debcd068e/IGVFFI4247LKDN.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S16-BioRep2-FFRep2-BinE-PCRrep1_S138_I2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI7161KOGZ/",
"accession": "IGVFFI7161KOGZ",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep2-FFrep2-BinE-PCRrep2_I2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T20:32:46.442326+00:00",
"file_format": "fastq",
"file_size": 3267564,
"href": "/sequence-files/IGVFFI7161KOGZ/@@download/IGVFFI7161KOGZ.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/c8d89f22-d708-4a28-bbe3-844c8d926380/IGVFFI7161KOGZ.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S16-BioRep2-FFRep2-BinE-PCRrep2_S195_I2_001.fastq.gz",
"upload_status": "validated"
}
],
"input_for": [
"/analysis-sets/IGVFDS1863TJOE/"
],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set. This measurement set previously used Variant FlowFISH as a preferred_assay_title, but this enum is now removed. So it has been defaulted to Variant-EFFECTS.",
"preferred_assay_title": "Variant-EFFECTS",
"protocols": [
"https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
],
"release_timestamp": "2023-12-20T23:04:24.244936+00:00",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM5988GKVB/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM5988GKVB",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep2-FFrep2-BinE"
],
"classifications": [
"cell line"
],
"construct_library_sets": [
{
"@id": "/construct-library-sets/IGVFDS6377KNCH/",
"accession": "IGVFDS6377KNCH",
"file_set_type": "guide library",
"status": "in progress",
"summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
}
],
"modifications": [
{
"@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
"modality": "prime editing",
"status": "released"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"@type": [
"SampleTerm",
"OntologyTerm",
"Item"
],
"aliases": [
"igvf:Jurkat"
],
"status": "released",
"summary": "Jurkat",
"term_name": "Jurkat"
}
],
"status": "released",
"summary": "Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 80-90% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
"taxa": "Homo sapiens",
"treatments": [
{
"@id": "/treatments/df02c0d4-f796-4f77-bfbc-388cdbfcc615/",
"status": "released",
"treatment_term_name": "T-cell surface glycoprotein CD3 gamma chain"
},
{
"@id": "/treatments/42b3c61d-38de-4bd2-bb39-33c125a417cb/",
"status": "released",
"treatment_term_name": "phorbol 13-acetate 12-myristate"
}
]
}
],
"schema_version": "23",
"status": "released",
"submitted_by": {
"@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
"title": "Michael Montgomery"
},
"submitted_files_timestamp": "2023-12-05T19:34:29.424303+00:00",
"summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
"targeted_genes": [
{
"@id": "/genes/ENSG00000108179/",
"geneid": "ENSG00000108179",
"name": "peptidylprolyl isomerase F",
"status": "released",
"symbol": "PPIF",
"synonyms": [
"rotamase F",
"CYP3",
"cyclophilin F",
"mitochondrial cyclophilin",
"peptidyl-prolyl cis-trans isomerase, mitochondrial",
"CypD",
"Cyp-D",
"cyclophilin 3",
"cyclophilin D",
"CyP-M",
"peptidyl-prolyl cis-trans isomerase F, mitochondrial",
"PPIase F"
]
}
],
"uuid": "574ad337-cf7d-4827-94d0-3776597a9935"
}