IGVFDS5913QKWA

released
{
    "@context": "/terms/",
    "@id": "/measurement-sets/IGVFDS5913QKWA/",
    "@type": [
        "MeasurementSet",
        "FileSet",
        "Item"
    ],
    "accession": "IGVFDS5913QKWA",
    "aliases": [
        "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep1-FFrep3-BinA_AmpliconSequencing"
    ],
    "assay_term": {
        "@id": "/assay-terms/OBI_0003661/",
        "status": "released",
        "term_name": "in vitro CRISPR screen using flow cytometry"
    },
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "mismatched status",
                "detail": "Released in vitro system [IGVFSM7453CDOR](/in-vitro-systems/IGVFSM7453CDOR/) has in progress subobject construct library set [IGVFDS2385DHZO](/construct-library-sets/IGVFDS2385DHZO/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/in-vitro-systems/IGVFSM7453CDOR/"
            },
            {
                "category": "missing sequencing kit",
                "detail": "Measurement set [IGVFDS5913QKWA](/measurement-sets/IGVFDS5913QKWA/) has sequence file(s) [IGVFFI5538EBRB](/sequence-files/IGVFFI5538EBRB/), [IGVFFI6606PLCG](/sequence-files/IGVFFI6606PLCG/), [IGVFFI4854CJNH](/sequence-files/IGVFFI4854CJNH/), [IGVFFI4880YDGX](/sequence-files/IGVFFI4880YDGX/), [IGVFFI0503TXAP](/sequence-files/IGVFFI0503TXAP/), [IGVFFI9261NDVX](/sequence-files/IGVFFI9261NDVX/), [IGVFFI0234KSKE](/sequence-files/IGVFFI0234KSKE/), [IGVFFI5830XVIE](/sequence-files/IGVFFI5830XVIE/), [IGVFFI8977YYWC](/sequence-files/IGVFFI8977YYWC/), [IGVFFI2982BLYB](/sequence-files/IGVFFI2982BLYB/), [IGVFFI1885WBEH](/sequence-files/IGVFFI1885WBEH/), [IGVFFI7184BYWH](/sequence-files/IGVFFI7184BYWH/), [IGVFFI5629WMGP](/sequence-files/IGVFFI5629WMGP/), [IGVFFI4664MANF](/sequence-files/IGVFFI4664MANF/), [IGVFFI5566BYRL](/sequence-files/IGVFFI5566BYRL/), [IGVFFI0405HATV](/sequence-files/IGVFFI0405HATV/), [IGVFFI3441EPFF](/sequence-files/IGVFFI3441EPFF/), [IGVFFI4689FPJI](/sequence-files/IGVFFI4689FPJI/), [IGVFFI7538WLJI](/sequence-files/IGVFFI7538WLJI/), [IGVFFI7416QOPH](/sequence-files/IGVFFI7416QOPH/), [IGVFFI8652HYNB](/sequence-files/IGVFFI8652HYNB/), [IGVFFI5533FKIL](/sequence-files/IGVFFI5533FKIL/), [IGVFFI5025AKHB](/sequence-files/IGVFFI5025AKHB/), [IGVFFI7490FCDL](/sequence-files/IGVFFI7490FCDL/), [IGVFFI9264QXPY](/sequence-files/IGVFFI9264QXPY/), [IGVFFI4190NQYP](/sequence-files/IGVFFI4190NQYP/), [IGVFFI9018SBSM](/sequence-files/IGVFFI9018SBSM/), [IGVFFI8450ZXUO](/sequence-files/IGVFFI8450ZXUO/), [IGVFFI5833XPZA](/sequence-files/IGVFFI5833XPZA/), [IGVFFI2088RXNC](/sequence-files/IGVFFI2088RXNC/), [IGVFFI2642HHPE](/sequence-files/IGVFFI2642HHPE/), [IGVFFI8165CTYL](/sequence-files/IGVFFI8165CTYL/), [IGVFFI3149GIXS](/sequence-files/IGVFFI3149GIXS/), [IGVFFI9788RCRL](/sequence-files/IGVFFI9788RCRL/), [IGVFFI8452SDOL](/sequence-files/IGVFFI8452SDOL/), [IGVFFI2060GXOJ](/sequence-files/IGVFFI2060GXOJ/), [IGVFFI6748GXQW](/sequence-files/IGVFFI6748GXQW/), [IGVFFI7614VLSQ](/sequence-files/IGVFFI7614VLSQ/), [IGVFFI2390FOKU](/sequence-files/IGVFFI2390FOKU/), [IGVFFI2003BZPY](/sequence-files/IGVFFI2003BZPY/), [IGVFFI0621COCJ](/sequence-files/IGVFFI0621COCJ/), [IGVFFI3376QDOE](/sequence-files/IGVFFI3376QDOE/), [IGVFFI2605SVTI](/sequence-files/IGVFFI2605SVTI/), [IGVFFI5441YEJA](/sequence-files/IGVFFI5441YEJA/), [IGVFFI2069BDCH](/sequence-files/IGVFFI2069BDCH/), [IGVFFI5728XYGQ](/sequence-files/IGVFFI5728XYGQ/), [IGVFFI6090XTHK](/sequence-files/IGVFFI6090XTHK/), [IGVFFI7951GGIK](/sequence-files/IGVFFI7951GGIK/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/measurement-sets/IGVFDS5913QKWA/"
            },
            {
                "category": "missing sequence specification",
                "detail": "Measurement set [IGVFDS5913QKWA](/measurement-sets/IGVFDS5913QKWA/) has sequence file(s): [IGVFFI5538EBRB](/sequence-files/IGVFFI5538EBRB/), [IGVFFI6606PLCG](/sequence-files/IGVFFI6606PLCG/), [IGVFFI4854CJNH](/sequence-files/IGVFFI4854CJNH/), [IGVFFI4880YDGX](/sequence-files/IGVFFI4880YDGX/), [IGVFFI0503TXAP](/sequence-files/IGVFFI0503TXAP/), [IGVFFI9261NDVX](/sequence-files/IGVFFI9261NDVX/), [IGVFFI0234KSKE](/sequence-files/IGVFFI0234KSKE/), [IGVFFI5830XVIE](/sequence-files/IGVFFI5830XVIE/), [IGVFFI8977YYWC](/sequence-files/IGVFFI8977YYWC/), [IGVFFI2982BLYB](/sequence-files/IGVFFI2982BLYB/), [IGVFFI1885WBEH](/sequence-files/IGVFFI1885WBEH/), [IGVFFI7184BYWH](/sequence-files/IGVFFI7184BYWH/), [IGVFFI5629WMGP](/sequence-files/IGVFFI5629WMGP/), [IGVFFI4664MANF](/sequence-files/IGVFFI4664MANF/), [IGVFFI5566BYRL](/sequence-files/IGVFFI5566BYRL/), [IGVFFI0405HATV](/sequence-files/IGVFFI0405HATV/), [IGVFFI3441EPFF](/sequence-files/IGVFFI3441EPFF/), [IGVFFI4689FPJI](/sequence-files/IGVFFI4689FPJI/), [IGVFFI7538WLJI](/sequence-files/IGVFFI7538WLJI/), [IGVFFI7416QOPH](/sequence-files/IGVFFI7416QOPH/), [IGVFFI8652HYNB](/sequence-files/IGVFFI8652HYNB/), [IGVFFI5533FKIL](/sequence-files/IGVFFI5533FKIL/), [IGVFFI5025AKHB](/sequence-files/IGVFFI5025AKHB/), [IGVFFI7490FCDL](/sequence-files/IGVFFI7490FCDL/), [IGVFFI9264QXPY](/sequence-files/IGVFFI9264QXPY/), [IGVFFI4190NQYP](/sequence-files/IGVFFI4190NQYP/), [IGVFFI9018SBSM](/sequence-files/IGVFFI9018SBSM/), [IGVFFI8450ZXUO](/sequence-files/IGVFFI8450ZXUO/), [IGVFFI5833XPZA](/sequence-files/IGVFFI5833XPZA/), [IGVFFI2088RXNC](/sequence-files/IGVFFI2088RXNC/), [IGVFFI2642HHPE](/sequence-files/IGVFFI2642HHPE/), [IGVFFI8165CTYL](/sequence-files/IGVFFI8165CTYL/), [IGVFFI3149GIXS](/sequence-files/IGVFFI3149GIXS/), [IGVFFI9788RCRL](/sequence-files/IGVFFI9788RCRL/), [IGVFFI8452SDOL](/sequence-files/IGVFFI8452SDOL/), [IGVFFI2060GXOJ](/sequence-files/IGVFFI2060GXOJ/), [IGVFFI6748GXQW](/sequence-files/IGVFFI6748GXQW/), [IGVFFI7614VLSQ](/sequence-files/IGVFFI7614VLSQ/), [IGVFFI2390FOKU](/sequence-files/IGVFFI2390FOKU/), [IGVFFI2003BZPY](/sequence-files/IGVFFI2003BZPY/), [IGVFFI0621COCJ](/sequence-files/IGVFFI0621COCJ/), [IGVFFI3376QDOE](/sequence-files/IGVFFI3376QDOE/), [IGVFFI2605SVTI](/sequence-files/IGVFFI2605SVTI/), [IGVFFI5441YEJA](/sequence-files/IGVFFI5441YEJA/), [IGVFFI2069BDCH](/sequence-files/IGVFFI2069BDCH/), [IGVFFI5728XYGQ](/sequence-files/IGVFFI5728XYGQ/), [IGVFFI6090XTHK](/sequence-files/IGVFFI6090XTHK/), [IGVFFI7951GGIK](/sequence-files/IGVFFI7951GGIK/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_missing_seqspec",
                "path": "/measurement-sets/IGVFDS5913QKWA/"
            }
        ],
        "WARNING": [
            {
                "category": "missing auxiliary set",
                "detail": "Measurement set [IGVFDS5913QKWA](/measurement-sets/IGVFDS5913QKWA/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_missing_auxiliary_set",
                "path": "/measurement-sets/IGVFDS5913QKWA/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization",
        "contact_pi": {
            "@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
            "title": "Jesse Engreitz"
        },
        "title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
    },
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            "@id": "/measurement-sets/IGVFDS4869FXYY/",
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                "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-Jurkat-PE2_Exp2_NegControl_AmpliconSequencing"
            ],
            "status": "released"
        }
    ],
    "control_for": [],
    "creation_timestamp": "2023-12-13T08:48:06.344965+00:00",
    "donors": [
        {
            "@id": "/human-donors/IGVFDO1080XFGV/",
            "accession": "IGVFDO1080XFGV",
            "aliases": [
                "igvf:donor_of_Jurkat"
            ],
            "sex": "male",
            "status": "released",
            "taxa": "Homo sapiens"
        }
    ],
    "externally_hosted": false,
    "file_set_type": "experimental data",
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            "@id": "/sequence-files/IGVFFI1885WBEH/",
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            "s3_uri": "s3://igvf-files/2023/12/13/b7768baf-86e7-49dd-8644-50c5f90831f8/IGVFFI7951GGIK.fastq.gz",
            "sequencing_platform": {
                "@id": "/platform-terms/EFO_0008566/",
                "status": "released",
                "term_name": "Illumina NextSeq 550"
            },
            "status": "released",
            "submitted_file_name": "230423pPPIFEnformerJurkat-Jurkat-PE-pPPIF_Enformer-Amp-pPPIF-BioRep1-FFRep3_BinA-PCRrep3_S99_R2_001.fastq.gz",
            "upload_status": "validated"
        }
    ],
    "input_for": [
        "/analysis-sets/IGVFDS6299IJHG/"
    ],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set. This measurement set previously used Variant FlowFISH as a preferred_assay_title, but this enum is now removed. So it has been defaulted to Variant-EFFECTS.",
    "preferred_assay_title": "Variant-EFFECTS",
    "protocols": [
        "https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
    ],
    "release_timestamp": "2023-12-20T23:08:50.443160+00:00",
    "samples": [
        {
            "@id": "/in-vitro-systems/IGVFSM7453CDOR/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM7453CDOR",
            "aliases": [
                "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep1-FFrep3-BinA"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                {
                    "@id": "/construct-library-sets/IGVFDS2385DHZO/",
                    "accession": "IGVFDS2385DHZO",
                    "file_set_type": "guide library",
                    "status": "in progress",
                    "summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
                }
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0002796/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:Jurkat"
                    ],
                    "status": "released",
                    "summary": "Jurkat",
                    "term_name": "Jurkat"
                }
            ],
            "status": "released",
            "summary": "Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        }
    ],
    "schema_version": "23",
    "status": "released",
    "submitted_by": {
        "@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
        "title": "Michael Montgomery"
    },
    "submitted_files_timestamp": "2023-12-13T09:44:21.813738+00:00",
    "summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
    "targeted_genes": [
        {
            "@id": "/genes/ENSG00000108179/",
            "geneid": "ENSG00000108179",
            "name": "peptidylprolyl isomerase F",
            "status": "released",
            "symbol": "PPIF",
            "synonyms": [
                "rotamase F",
                "CYP3",
                "cyclophilin F",
                "mitochondrial cyclophilin",
                "peptidyl-prolyl cis-trans isomerase, mitochondrial",
                "CypD",
                "Cyp-D",
                "cyclophilin 3",
                "cyclophilin D",
                "CyP-M",
                "peptidyl-prolyl cis-trans isomerase F, mitochondrial",
                "PPIase F"
            ]
        }
    ],
    "uuid": "d49b8a0c-bcfe-44d9-a53c-786dd3bf2448"
}