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IGVFDS5864UGFT

released
File Set Type
experimental data
Summary
untransfected prime editing Variant-EFFECTS
Assay Term
in vitro CRISPR screen using flow cytometry
Sample Summaries
  • THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)
Protocols
Aliases
jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-THP1-PE2_Exp2_NegControl_AmpliconSequencing

Sequencing Results (Illumina)

16 items
fastq
reads
I2
1
TGTAATCA:TTGT
1.2 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
TGTAATCA:TTGT
5.0 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
GTAACATC:TTGT
1.2 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
CTAACTCG:TTGT
1.2 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
CTAACTCG:TTGT
3.9 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
CTAACTCG:TTGT
1.2 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
GTAACATC:TTGT
1.2 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
CTAACTCG:TTGT
5.1 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
GTAACATC:TTGT
3.9 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
TGTAATCA:TTGT
3.9 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_THP1
male
Homo sapiens

File Sets Using This Measurement Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
prime editing Variant-EFFECTS targeting PPIF: variant effects
  • jesse-engreitz:PPIF_promoter_tiling_THP1_Variant-FlowFISH_analysis
Jesse Engreitz, Stanford

File Sets Controlled by This Measurement Set

40 items
MeasurementSet
unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep4-FACS_Input_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep1-BinB_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep2-FFrep4-FACS_Input_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep2-FFrep4-BinA_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep2-FFrep3-BinA_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep1-BinA_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep4-BinB_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep2-FFrep2-BinB_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep2-FFrep3-BinB_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep1-FACS_Input_AmpliconSequencing
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford