IGVFDS5856FZKF

released
{
    "@context": "/terms/",
    "@id": "/measurement-sets/IGVFDS5856FZKF/",
    "@type": [
        "MeasurementSet",
        "FileSet",
        "Item"
    ],
    "accession": "IGVFDS5856FZKF",
    "aliases": [
        "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep2-FFrep2-BinB_AmpliconSequencing"
    ],
    "assay_term": {
        "@id": "/assay-terms/OBI_0003661/",
        "status": "released",
        "term_name": "in vitro CRISPR screen using flow cytometry"
    },
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "missing sequencing kit",
                "detail": "Measurement set [IGVFDS5856FZKF](/measurement-sets/IGVFDS5856FZKF/) has sequence file(s) [IGVFFI4047BBPJ](/sequence-files/IGVFFI4047BBPJ/), [IGVFFI6397SUJC](/sequence-files/IGVFFI6397SUJC/), [IGVFFI5018WXNV](/sequence-files/IGVFFI5018WXNV/), [IGVFFI0683KABS](/sequence-files/IGVFFI0683KABS/), [IGVFFI3260ODND](/sequence-files/IGVFFI3260ODND/), [IGVFFI1815CRNJ](/sequence-files/IGVFFI1815CRNJ/), [IGVFFI2823RWAW](/sequence-files/IGVFFI2823RWAW/), [IGVFFI1020OWEB](/sequence-files/IGVFFI1020OWEB/), [IGVFFI6845LMPY](/sequence-files/IGVFFI6845LMPY/), [IGVFFI7830NIBI](/sequence-files/IGVFFI7830NIBI/), [IGVFFI7347GBNW](/sequence-files/IGVFFI7347GBNW/), [IGVFFI9237NRDU](/sequence-files/IGVFFI9237NRDU/), [IGVFFI3549ITFF](/sequence-files/IGVFFI3549ITFF/), [IGVFFI1023NAVB](/sequence-files/IGVFFI1023NAVB/), [IGVFFI4791XXDL](/sequence-files/IGVFFI4791XXDL/), [IGVFFI6478TGPB](/sequence-files/IGVFFI6478TGPB/), [IGVFFI9505ZJTP](/sequence-files/IGVFFI9505ZJTP/), [IGVFFI3463QFUD](/sequence-files/IGVFFI3463QFUD/), [IGVFFI4859XPET](/sequence-files/IGVFFI4859XPET/), [IGVFFI1282FWJQ](/sequence-files/IGVFFI1282FWJQ/), [IGVFFI4721AXTO](/sequence-files/IGVFFI4721AXTO/), [IGVFFI6748XRYL](/sequence-files/IGVFFI6748XRYL/), [IGVFFI2520CEXS](/sequence-files/IGVFFI2520CEXS/), [IGVFFI2427CLUI](/sequence-files/IGVFFI2427CLUI/), [IGVFFI5551STSB](/sequence-files/IGVFFI5551STSB/), [IGVFFI1458HQEB](/sequence-files/IGVFFI1458HQEB/), [IGVFFI7502LMVQ](/sequence-files/IGVFFI7502LMVQ/), [IGVFFI1560IQYJ](/sequence-files/IGVFFI1560IQYJ/), [IGVFFI0578DORF](/sequence-files/IGVFFI0578DORF/), [IGVFFI6029OCBI](/sequence-files/IGVFFI6029OCBI/), [IGVFFI1070KTCG](/sequence-files/IGVFFI1070KTCG/), [IGVFFI9545EZRY](/sequence-files/IGVFFI9545EZRY/), [IGVFFI7429CIJF](/sequence-files/IGVFFI7429CIJF/), [IGVFFI2031SKIL](/sequence-files/IGVFFI2031SKIL/), [IGVFFI2351TLFO](/sequence-files/IGVFFI2351TLFO/), [IGVFFI6533YLMJ](/sequence-files/IGVFFI6533YLMJ/), [IGVFFI7734RLWM](/sequence-files/IGVFFI7734RLWM/), [IGVFFI4402QMQD](/sequence-files/IGVFFI4402QMQD/), [IGVFFI4075FCRP](/sequence-files/IGVFFI4075FCRP/), [IGVFFI7199EJUV](/sequence-files/IGVFFI7199EJUV/), [IGVFFI3158RNKH](/sequence-files/IGVFFI3158RNKH/), [IGVFFI6263BSHT](/sequence-files/IGVFFI6263BSHT/), [IGVFFI6735AEPI](/sequence-files/IGVFFI6735AEPI/), [IGVFFI1130XONB](/sequence-files/IGVFFI1130XONB/), [IGVFFI4296KZTZ](/sequence-files/IGVFFI4296KZTZ/), [IGVFFI7113WZPT](/sequence-files/IGVFFI7113WZPT/), [IGVFFI5742CFJG](/sequence-files/IGVFFI5742CFJG/), [IGVFFI1145KNZE](/sequence-files/IGVFFI1145KNZE/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/measurement-sets/IGVFDS5856FZKF/"
            },
            {
                "category": "missing sequence specification",
                "detail": "Measurement set [IGVFDS5856FZKF](/measurement-sets/IGVFDS5856FZKF/) has sequence file(s): [IGVFFI4047BBPJ](/sequence-files/IGVFFI4047BBPJ/), [IGVFFI6397SUJC](/sequence-files/IGVFFI6397SUJC/), [IGVFFI5018WXNV](/sequence-files/IGVFFI5018WXNV/), [IGVFFI0683KABS](/sequence-files/IGVFFI0683KABS/), [IGVFFI3260ODND](/sequence-files/IGVFFI3260ODND/), [IGVFFI1815CRNJ](/sequence-files/IGVFFI1815CRNJ/), [IGVFFI2823RWAW](/sequence-files/IGVFFI2823RWAW/), [IGVFFI1020OWEB](/sequence-files/IGVFFI1020OWEB/), [IGVFFI6845LMPY](/sequence-files/IGVFFI6845LMPY/), [IGVFFI7830NIBI](/sequence-files/IGVFFI7830NIBI/), [IGVFFI7347GBNW](/sequence-files/IGVFFI7347GBNW/), [IGVFFI9237NRDU](/sequence-files/IGVFFI9237NRDU/), [IGVFFI3549ITFF](/sequence-files/IGVFFI3549ITFF/), [IGVFFI1023NAVB](/sequence-files/IGVFFI1023NAVB/), [IGVFFI4791XXDL](/sequence-files/IGVFFI4791XXDL/), [IGVFFI6478TGPB](/sequence-files/IGVFFI6478TGPB/), [IGVFFI9505ZJTP](/sequence-files/IGVFFI9505ZJTP/), [IGVFFI3463QFUD](/sequence-files/IGVFFI3463QFUD/), [IGVFFI4859XPET](/sequence-files/IGVFFI4859XPET/), [IGVFFI1282FWJQ](/sequence-files/IGVFFI1282FWJQ/), [IGVFFI4721AXTO](/sequence-files/IGVFFI4721AXTO/), [IGVFFI6748XRYL](/sequence-files/IGVFFI6748XRYL/), [IGVFFI2520CEXS](/sequence-files/IGVFFI2520CEXS/), [IGVFFI2427CLUI](/sequence-files/IGVFFI2427CLUI/), [IGVFFI5551STSB](/sequence-files/IGVFFI5551STSB/), [IGVFFI1458HQEB](/sequence-files/IGVFFI1458HQEB/), [IGVFFI7502LMVQ](/sequence-files/IGVFFI7502LMVQ/), [IGVFFI1560IQYJ](/sequence-files/IGVFFI1560IQYJ/), [IGVFFI0578DORF](/sequence-files/IGVFFI0578DORF/), [IGVFFI6029OCBI](/sequence-files/IGVFFI6029OCBI/), [IGVFFI1070KTCG](/sequence-files/IGVFFI1070KTCG/), [IGVFFI9545EZRY](/sequence-files/IGVFFI9545EZRY/), [IGVFFI7429CIJF](/sequence-files/IGVFFI7429CIJF/), [IGVFFI2031SKIL](/sequence-files/IGVFFI2031SKIL/), [IGVFFI2351TLFO](/sequence-files/IGVFFI2351TLFO/), [IGVFFI6533YLMJ](/sequence-files/IGVFFI6533YLMJ/), [IGVFFI7734RLWM](/sequence-files/IGVFFI7734RLWM/), [IGVFFI4402QMQD](/sequence-files/IGVFFI4402QMQD/), [IGVFFI4075FCRP](/sequence-files/IGVFFI4075FCRP/), [IGVFFI7199EJUV](/sequence-files/IGVFFI7199EJUV/), [IGVFFI3158RNKH](/sequence-files/IGVFFI3158RNKH/), [IGVFFI6263BSHT](/sequence-files/IGVFFI6263BSHT/), [IGVFFI6735AEPI](/sequence-files/IGVFFI6735AEPI/), [IGVFFI1130XONB](/sequence-files/IGVFFI1130XONB/), [IGVFFI4296KZTZ](/sequence-files/IGVFFI4296KZTZ/), [IGVFFI7113WZPT](/sequence-files/IGVFFI7113WZPT/), [IGVFFI5742CFJG](/sequence-files/IGVFFI5742CFJG/), [IGVFFI1145KNZE](/sequence-files/IGVFFI1145KNZE/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_missing_seqspec",
                "path": "/measurement-sets/IGVFDS5856FZKF/"
            },
            {
                "category": "mismatched status",
                "detail": "Released in vitro system [IGVFSM7961LTZR](/in-vitro-systems/IGVFSM7961LTZR/) has in progress subobject construct library set [IGVFDS2385DHZO](/construct-library-sets/IGVFDS2385DHZO/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/in-vitro-systems/IGVFSM7961LTZR/"
            }
        ],
        "WARNING": [
            {
                "category": "missing auxiliary set",
                "detail": "Measurement set [IGVFDS5856FZKF](/measurement-sets/IGVFDS5856FZKF/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_missing_auxiliary_set",
                "path": "/measurement-sets/IGVFDS5856FZKF/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization",
        "contact_pi": {
            "@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
            "title": "Jesse Engreitz"
        },
        "title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
    },
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                "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-Jurkat-PE2_Exp2_NegControl_AmpliconSequencing"
            ],
            "status": "released"
        }
    ],
    "control_for": [],
    "creation_timestamp": "2023-12-13T08:48:11.854861+00:00",
    "donors": [
        {
            "@id": "/human-donors/IGVFDO1080XFGV/",
            "accession": "IGVFDO1080XFGV",
            "aliases": [
                "igvf:donor_of_Jurkat"
            ],
            "sex": "male",
            "status": "released",
            "taxa": "Homo sapiens"
        }
    ],
    "externally_hosted": false,
    "file_set_type": "experimental data",
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            "s3_uri": "s3://igvf-files/2023/12/13/56a9d31a-8ea3-4541-b85b-7885142ba480/IGVFFI1145KNZE.fastq.gz",
            "sequencing_platform": {
                "@id": "/platform-terms/EFO_0008566/",
                "status": "released",
                "term_name": "Illumina NextSeq 550"
            },
            "status": "released",
            "submitted_file_name": "230423pPPIFEnformerJurkat-Jurkat-PE-pPPIF_Enformer-Amp-pPPIF-BioRep2-FFRep2_BinB-PCRrep3_S255_I2_001.fastq.gz",
            "upload_status": "validated"
        }
    ],
    "input_for": [
        "/analysis-sets/IGVFDS6299IJHG/"
    ],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set. This measurement set previously used Variant FlowFISH as a preferred_assay_title, but this enum is now removed. So it has been defaulted to Variant-EFFECTS.",
    "preferred_assay_title": "Variant-EFFECTS",
    "protocols": [
        "https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
    ],
    "release_timestamp": "2023-12-20T23:08:41.966536+00:00",
    "samples": [
        {
            "@id": "/in-vitro-systems/IGVFSM7961LTZR/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM7961LTZR",
            "aliases": [
                "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep2-FFrep2-BinB"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                {
                    "@id": "/construct-library-sets/IGVFDS2385DHZO/",
                    "accession": "IGVFDS2385DHZO",
                    "file_set_type": "guide library",
                    "status": "in progress",
                    "summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
                }
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0002796/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:Jurkat"
                    ],
                    "status": "released",
                    "summary": "Jurkat",
                    "term_name": "Jurkat"
                }
            ],
            "status": "released",
            "summary": "Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 26-50% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        }
    ],
    "schema_version": "23",
    "status": "released",
    "submitted_by": {
        "@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
        "title": "Michael Montgomery"
    },
    "submitted_files_timestamp": "2023-12-13T10:08:31.141544+00:00",
    "summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
    "targeted_genes": [
        {
            "@id": "/genes/ENSG00000108179/",
            "geneid": "ENSG00000108179",
            "name": "peptidylprolyl isomerase F",
            "status": "released",
            "symbol": "PPIF",
            "synonyms": [
                "rotamase F",
                "CYP3",
                "cyclophilin F",
                "mitochondrial cyclophilin",
                "peptidyl-prolyl cis-trans isomerase, mitochondrial",
                "CypD",
                "Cyp-D",
                "cyclophilin 3",
                "cyclophilin D",
                "CyP-M",
                "peptidyl-prolyl cis-trans isomerase F, mitochondrial",
                "PPIase F"
            ]
        }
    ],
    "uuid": "6c8ecad6-445f-4b0a-b1ab-aea54add7456"
}