IGVFDS5847JIJX

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

MYC

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Construct Library Sets

IGVFDS7857SYCK guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR1_Rep1_S29

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR1_Rep2_S84

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR2_Rep1_S35

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR2_Rep2_S90

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR3_Rep1_S41

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR3_Rep2_S96

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR4_Rep1_S47

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR4_Rep2_S102

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM1124JJAA

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS2059NFJA

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI4334TLKF, IGVFFI8214NGYS, IGVFFI2421MJBY, IGVFFI0157HGQY, IGVFFI6105CYOK, IGVFFI2543THHO, IGVFFI2477AUWQ, IGVFFI1421XINF

jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR1_Rep1_S29
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR1_Rep2_S84
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR2_Rep1_S35
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR2_Rep2_S90
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR3_Rep1_S41
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR3_Rep2_S96
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR4_Rep1_S47
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR4_Rep2_S102

Jesse Engreitz, Stanford

IGVFDS8214HXSU

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI3328SYVP