IGVFDS5733FUWG

released
{
    "@context": "/terms/",
    "@id": "/measurement-sets/IGVFDS5733FUWG/",
    "@type": [
        "MeasurementSet",
        "FileSet",
        "Item"
    ],
    "accession": "IGVFDS5733FUWG",
    "aliases": [
        "jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep3-FACS_Input_AmpliconSequencing"
    ],
    "assay_term": {
        "@id": "/assay-terms/OBI_0003661/",
        "assay_slims": [
            "CRISPR screens"
        ],
        "status": "released",
        "term_name": "in vitro CRISPR screen using flow cytometry"
    },
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "missing publication",
                "detail": "Measurement set [IGVFDS5733FUWG](/measurement-sets/IGVFDS5733FUWG/) has no `publications`. Released and archived file sets are expected to be associated with a publication.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_file_set_missing_publication",
                "path": "/measurement-sets/IGVFDS5733FUWG/"
            },
            {
                "category": "missing sequencing kit",
                "detail": "Measurement set [IGVFDS5733FUWG](/measurement-sets/IGVFDS5733FUWG/) has sequence file(s) [IGVFFI4746YCMV](/sequence-files/IGVFFI4746YCMV/), [IGVFFI8695JDBM](/sequence-files/IGVFFI8695JDBM/), [IGVFFI7946MOUL](/sequence-files/IGVFFI7946MOUL/), [IGVFFI0658RYQK](/sequence-files/IGVFFI0658RYQK/), [IGVFFI8972DZDR](/sequence-files/IGVFFI8972DZDR/), [IGVFFI3653ZRMB](/sequence-files/IGVFFI3653ZRMB/), [IGVFFI5600OSJT](/sequence-files/IGVFFI5600OSJT/), [IGVFFI1222KZLK](/sequence-files/IGVFFI1222KZLK/), [IGVFFI1719NHSE](/sequence-files/IGVFFI1719NHSE/), [IGVFFI2746TMJP](/sequence-files/IGVFFI2746TMJP/), [IGVFFI6376GTQV](/sequence-files/IGVFFI6376GTQV/), [IGVFFI9019NHRM](/sequence-files/IGVFFI9019NHRM/), [IGVFFI2996GNNA](/sequence-files/IGVFFI2996GNNA/), [IGVFFI3336SUCZ](/sequence-files/IGVFFI3336SUCZ/), [IGVFFI5541ETUF](/sequence-files/IGVFFI5541ETUF/), [IGVFFI4143FBNZ](/sequence-files/IGVFFI4143FBNZ/) which lack specification of a `sequencing_kit`. Sequence files in a file set associated wtih bulk data should specify a sequencing kit.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/measurement-sets/IGVFDS5733FUWG/"
            },
            {
                "category": "missing sequence specification",
                "detail": "Measurement set [IGVFDS5733FUWG](/measurement-sets/IGVFDS5733FUWG/) has sequence file(s): [IGVFFI4746YCMV](/sequence-files/IGVFFI4746YCMV/), [IGVFFI8695JDBM](/sequence-files/IGVFFI8695JDBM/), [IGVFFI7946MOUL](/sequence-files/IGVFFI7946MOUL/), [IGVFFI0658RYQK](/sequence-files/IGVFFI0658RYQK/), [IGVFFI8972DZDR](/sequence-files/IGVFFI8972DZDR/), [IGVFFI3653ZRMB](/sequence-files/IGVFFI3653ZRMB/), [IGVFFI5600OSJT](/sequence-files/IGVFFI5600OSJT/), [IGVFFI1222KZLK](/sequence-files/IGVFFI1222KZLK/), [IGVFFI1719NHSE](/sequence-files/IGVFFI1719NHSE/), [IGVFFI2746TMJP](/sequence-files/IGVFFI2746TMJP/), [IGVFFI6376GTQV](/sequence-files/IGVFFI6376GTQV/), [IGVFFI9019NHRM](/sequence-files/IGVFFI9019NHRM/), [IGVFFI2996GNNA](/sequence-files/IGVFFI2996GNNA/), [IGVFFI3336SUCZ](/sequence-files/IGVFFI3336SUCZ/), [IGVFFI5541ETUF](/sequence-files/IGVFFI5541ETUF/), [IGVFFI4143FBNZ](/sequence-files/IGVFFI4143FBNZ/) which do not have any `seqspecs`. Sequence files in a file set associated with bulk data are expected to link to a sequence specification file.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_missing_seqspec",
                "path": "/measurement-sets/IGVFDS5733FUWG/"
            },
            {
                "category": "mismatched status",
                "detail": "Released in vitro system [IGVFSM3818KKHR](/in-vitro-systems/IGVFSM3818KKHR/) has in progress subobject construct library set [IGVFDS6982DESN](/construct-library-sets/IGVFDS6982DESN/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/in-vitro-systems/IGVFSM3818KKHR/"
            },
            {
                "category": "missing publication",
                "detail": "In vitro system [IGVFSM3818KKHR](/in-vitro-systems/IGVFSM3818KKHR/) has no `publications`. Released and archived samples are expected to be associated with a publication.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_sample_missing_publication",
                "path": "/in-vitro-systems/IGVFSM3818KKHR/"
            }
        ],
        "NOT_COMPLIANT": [
            {
                "category": "missing control for",
                "detail": "Measurement set [IGVFDS5733FUWG](/measurement-sets/IGVFDS5733FUWG/) has no `control_for`. The `control_file_sets` should be patched on the file sets it serves as a control for and this property, `control_for`, will be reverse calculated. File sets that specify a control type are expected to be a control for other file sets.",
                "level": 50,
                "level_name": "NOT_COMPLIANT",
                "name": "audit_control_for_control_type",
                "path": "/measurement-sets/IGVFDS5733FUWG/"
            }
        ],
        "WARNING": [
            {
                "category": "missing auxiliary set",
                "detail": "Measurement set [IGVFDS5733FUWG](/measurement-sets/IGVFDS5733FUWG/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_missing_auxiliary_set",
                "path": "/measurement-sets/IGVFDS5733FUWG/"
            },
            {
                "category": "missing targeted genes",
                "detail": "Measurement set [IGVFDS5733FUWG](/measurement-sets/IGVFDS5733FUWG/) has no `targeted_genes`. ChIP-seq and CRISPR flow cytometry assays are expected to specify targeted gene(s).",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_targeted_genes",
                "path": "/measurement-sets/IGVFDS5733FUWG/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization",
        "contact_pi": {
            "@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
            "title": "Jesse Engreitz"
        },
        "title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
    },
    "construct_library_sets": [
        {
            "@id": "/construct-library-sets/IGVFDS6982DESN/",
            "accession": "IGVFDS6982DESN",
            "file_set_type": "guide library",
            "status": "in progress",
            "summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
        }
    ],
    "control_file_sets": [
        {
            "@id": "/measurement-sets/IGVFDS5864UGFT/",
            "accession": "IGVFDS5864UGFT",
            "aliases": [
                "jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-THP1-PE2_Exp2_NegControl_AmpliconSequencing"
            ],
            "status": "released"
        }
    ],
    "control_for": [],
    "control_type": "unsorted FACS input",
    "creation_timestamp": "2023-11-30T22:49:42.751617+00:00",
    "data_use_limitation_summaries": [
        "no certificate"
    ],
    "donors": [
        {
            "@id": "/human-donors/IGVFDO7359LVPI/",
            "accession": "IGVFDO7359LVPI",
            "aliases": [
                "igvf:donor_of_THP1"
            ],
            "sex": "male",
            "status": "released",
            "taxa": "Homo sapiens"
        }
    ],
    "externally_hosted": false,
    "file_set_type": "experimental data",
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            "@id": "/sequence-files/IGVFFI4746YCMV/",
            "accession": "IGVFFI4746YCMV",
            "aliases": [
                "jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep3-FACS_Input-PCRrep2_I2"
            ],
            "content_type": "reads",
            "controlled_access": false,
            "creation_timestamp": "2023-12-05T20:37:28.086983+00:00",
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            "file_size": 1289015,
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            "md5sum": "e7b6b5b0be88c3ec01876297241443c5",
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            "sequencing_platform": {
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                "status": "released",
                "term_name": "Illumina NextSeq 550"
            },
            "status": "released",
            "submitted_file_name": "230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep3-BinAll-PCRrep2_S396_I2_001.fastq.gz",
            "upload_status": "validated"
        },
        {
            "@id": "/sequence-files/IGVFFI8695JDBM/",
            "accession": "IGVFFI8695JDBM",
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                "jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep3-FACS_Input-PCRrep1_R1"
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            "upload_status": "validated"
        },
        {
            "@id": "/sequence-files/IGVFFI7946MOUL/",
            "accession": "IGVFFI7946MOUL",
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                "jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep3-FACS_Input-PCRrep4_R2"
            ],
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            "upload_status": "validated"
        },
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            "@id": "/sequence-files/IGVFFI0658RYQK/",
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            ],
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            "upload_status": "validated"
        },
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            "@id": "/sequence-files/IGVFFI8972DZDR/",
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            "upload_status": "validated"
        },
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            "@id": "/sequence-files/IGVFFI3653ZRMB/",
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            "upload_status": "validated"
        },
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            "@id": "/sequence-files/IGVFFI5600OSJT/",
            "accession": "IGVFFI5600OSJT",
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                "jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep3-FACS_Input-PCRrep4_I1"
            ],
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            "upload_status": "validated"
        },
        {
            "@id": "/sequence-files/IGVFFI1222KZLK/",
            "accession": "IGVFFI1222KZLK",
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                "jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep3-FACS_Input-PCRrep3_R2"
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        },
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            "@id": "/sequence-files/IGVFFI1719NHSE/",
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            "aliases": [
                "jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep3-FACS_Input-PCRrep4_R1"
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            "upload_status": "validated"
        },
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            "@id": "/sequence-files/IGVFFI2746TMJP/",
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        },
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            "upload_status": "validated"
        },
        {
            "@id": "/sequence-files/IGVFFI9019NHRM/",
            "accession": "IGVFFI9019NHRM",
            "aliases": [
                "jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep3-FACS_Input-PCRrep3_I1"
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    "protocols": [
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    ],
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}