{
"@context": "/terms/",
"@id": "/measurement-sets/IGVFDS5733FUWG/",
"@type": [
"MeasurementSet",
"FileSet",
"Item"
],
"accession": "IGVFDS5733FUWG",
"aliases": [
"jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep3-FACS_Input_AmpliconSequencing"
],
"assay_term": {
"@id": "/assay-terms/OBI_0003661/",
"assay_slims": [
"CRISPR screens"
],
"status": "released",
"term_name": "in vitro CRISPR screen using flow cytometry"
},
"audit": {
"INTERNAL_ACTION": [
{
"category": "missing publication",
"detail": "Measurement set [IGVFDS5733FUWG](/measurement-sets/IGVFDS5733FUWG/) has no `publications`. Released and archived file sets are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_file_set_missing_publication",
"path": "/measurement-sets/IGVFDS5733FUWG/"
},
{
"category": "missing sequencing kit",
"detail": "Measurement set [IGVFDS5733FUWG](/measurement-sets/IGVFDS5733FUWG/) has sequence file(s) [IGVFFI4746YCMV](/sequence-files/IGVFFI4746YCMV/), [IGVFFI8695JDBM](/sequence-files/IGVFFI8695JDBM/), [IGVFFI7946MOUL](/sequence-files/IGVFFI7946MOUL/), [IGVFFI0658RYQK](/sequence-files/IGVFFI0658RYQK/), [IGVFFI8972DZDR](/sequence-files/IGVFFI8972DZDR/), [IGVFFI3653ZRMB](/sequence-files/IGVFFI3653ZRMB/), [IGVFFI5600OSJT](/sequence-files/IGVFFI5600OSJT/), [IGVFFI1222KZLK](/sequence-files/IGVFFI1222KZLK/), [IGVFFI1719NHSE](/sequence-files/IGVFFI1719NHSE/), [IGVFFI2746TMJP](/sequence-files/IGVFFI2746TMJP/), [IGVFFI6376GTQV](/sequence-files/IGVFFI6376GTQV/), [IGVFFI9019NHRM](/sequence-files/IGVFFI9019NHRM/), [IGVFFI2996GNNA](/sequence-files/IGVFFI2996GNNA/), [IGVFFI3336SUCZ](/sequence-files/IGVFFI3336SUCZ/), [IGVFFI5541ETUF](/sequence-files/IGVFFI5541ETUF/), [IGVFFI4143FBNZ](/sequence-files/IGVFFI4143FBNZ/) which lack specification of a `sequencing_kit`. Sequence files in a file set associated wtih bulk data should specify a sequencing kit.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_inconsistent_sequencing_kit",
"path": "/measurement-sets/IGVFDS5733FUWG/"
},
{
"category": "missing sequence specification",
"detail": "Measurement set [IGVFDS5733FUWG](/measurement-sets/IGVFDS5733FUWG/) has sequence file(s): [IGVFFI4746YCMV](/sequence-files/IGVFFI4746YCMV/), [IGVFFI8695JDBM](/sequence-files/IGVFFI8695JDBM/), [IGVFFI7946MOUL](/sequence-files/IGVFFI7946MOUL/), [IGVFFI0658RYQK](/sequence-files/IGVFFI0658RYQK/), [IGVFFI8972DZDR](/sequence-files/IGVFFI8972DZDR/), [IGVFFI3653ZRMB](/sequence-files/IGVFFI3653ZRMB/), [IGVFFI5600OSJT](/sequence-files/IGVFFI5600OSJT/), [IGVFFI1222KZLK](/sequence-files/IGVFFI1222KZLK/), [IGVFFI1719NHSE](/sequence-files/IGVFFI1719NHSE/), [IGVFFI2746TMJP](/sequence-files/IGVFFI2746TMJP/), [IGVFFI6376GTQV](/sequence-files/IGVFFI6376GTQV/), [IGVFFI9019NHRM](/sequence-files/IGVFFI9019NHRM/), [IGVFFI2996GNNA](/sequence-files/IGVFFI2996GNNA/), [IGVFFI3336SUCZ](/sequence-files/IGVFFI3336SUCZ/), [IGVFFI5541ETUF](/sequence-files/IGVFFI5541ETUF/), [IGVFFI4143FBNZ](/sequence-files/IGVFFI4143FBNZ/) which do not have any `seqspecs`. Sequence files in a file set associated with bulk data are expected to link to a sequence specification file.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_missing_seqspec",
"path": "/measurement-sets/IGVFDS5733FUWG/"
},
{
"category": "mismatched status",
"detail": "Released in vitro system [IGVFSM3818KKHR](/in-vitro-systems/IGVFSM3818KKHR/) has in progress subobject construct library set [IGVFDS6982DESN](/construct-library-sets/IGVFDS6982DESN/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/in-vitro-systems/IGVFSM3818KKHR/"
},
{
"category": "missing publication",
"detail": "In vitro system [IGVFSM3818KKHR](/in-vitro-systems/IGVFSM3818KKHR/) has no `publications`. Released and archived samples are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_sample_missing_publication",
"path": "/in-vitro-systems/IGVFSM3818KKHR/"
}
],
"NOT_COMPLIANT": [
{
"category": "missing control for",
"detail": "Measurement set [IGVFDS5733FUWG](/measurement-sets/IGVFDS5733FUWG/) has no `control_for`. The `control_file_sets` should be patched on the file sets it serves as a control for and this property, `control_for`, will be reverse calculated. File sets that specify a control type are expected to be a control for other file sets.",
"level": 50,
"level_name": "NOT_COMPLIANT",
"name": "audit_control_for_control_type",
"path": "/measurement-sets/IGVFDS5733FUWG/"
}
],
"WARNING": [
{
"category": "missing auxiliary set",
"detail": "Measurement set [IGVFDS5733FUWG](/measurement-sets/IGVFDS5733FUWG/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
"level": 40,
"level_name": "WARNING",
"name": "audit_missing_auxiliary_set",
"path": "/measurement-sets/IGVFDS5733FUWG/"
},
{
"category": "missing targeted genes",
"detail": "Measurement set [IGVFDS5733FUWG](/measurement-sets/IGVFDS5733FUWG/) has no `targeted_genes`. ChIP-seq and CRISPR flow cytometry assays are expected to specify targeted gene(s).",
"level": 40,
"level_name": "WARNING",
"name": "audit_targeted_genes",
"path": "/measurement-sets/IGVFDS5733FUWG/"
}
]
},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
"title": "Jesse Engreitz"
},
"title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
},
"construct_library_sets": [
{
"@id": "/construct-library-sets/IGVFDS6982DESN/",
"accession": "IGVFDS6982DESN",
"file_set_type": "guide library",
"status": "in progress",
"summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
}
],
"control_file_sets": [
{
"@id": "/measurement-sets/IGVFDS5864UGFT/",
"accession": "IGVFDS5864UGFT",
"aliases": [
"jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-THP1-PE2_Exp2_NegControl_AmpliconSequencing"
],
"status": "released"
}
],
"control_for": [],
"control_type": "unsorted FACS input",
"creation_timestamp": "2023-11-30T22:49:42.751617+00:00",
"data_use_limitation_summaries": [
"no certificate"
],
"donors": [
{
"@id": "/human-donors/IGVFDO7359LVPI/",
"accession": "IGVFDO7359LVPI",
"aliases": [
"igvf:donor_of_THP1"
],
"sex": "male",
"status": "released",
"taxa": "Homo sapiens"
}
],
"externally_hosted": false,
"file_set_type": "experimental data",
"files": [
{
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"accession": "IGVFFI4746YCMV",
"aliases": [
"jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep3-FACS_Input-PCRrep2_I2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T20:37:28.086983+00:00",
"file_format": "fastq",
"file_size": 1289015,
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"md5sum": "e7b6b5b0be88c3ec01876297241443c5",
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"sequencing_platform": {
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"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep3-BinAll-PCRrep2_S396_I2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI8695JDBM/",
"accession": "IGVFFI8695JDBM",
"aliases": [
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],
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},
{
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],
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},
{
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],
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},
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"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI8972DZDR/",
"accession": "IGVFFI8972DZDR",
"aliases": [
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],
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},
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},
{
"@id": "/sequence-files/IGVFFI3653ZRMB/",
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],
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},
{
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],
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},
{
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},
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},
{
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],
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},
{
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],
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},
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},
{
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],
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},
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},
{
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],
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