IGVFDS5612XEIT

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

FAM3C

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Construct Library Sets

IGVFDS1891GTLU guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_FAM3C_Auxin0hrs-FF1-BinE-PCR1-Rep1_S113

jesse-engreitz:measurement_CRUDO_FAM3C_Auxin0hrs-FF1-BinE-PCR2-Rep1_S119

jesse-engreitz:measurement_CRUDO_FAM3C_Auxin0hrs-FF1-BinE-PCR3-Rep1_S167

jesse-engreitz:measurement_CRUDO_FAM3C_Auxin0hrs-FF1-BinE-PCR4-Rep1_S173

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM4222TCYN

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS3611WVHW

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI8497NPVR, IGVFFI0840FMQW, IGVFFI7555FXHU, IGVFFI0393VYGB

jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin0hrs-FF1-BinE-PCR1-Rep1_S113
jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin0hrs-FF1-BinE-PCR2-Rep1_S119
jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin0hrs-FF1-BinE-PCR3-Rep1_S167
jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin0hrs-FF1-BinE-PCR4-Rep1_S173

Jesse Engreitz, Stanford

IGVFDS7833JNUC

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI2396MZVU