{
"@context": "/terms/",
"@id": "/measurement-sets/IGVFDS5594RPYL/",
"@type": [
"MeasurementSet",
"FileSet",
"Item"
],
"accession": "IGVFDS5594RPYL",
"aliases": [
"jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep2-FFrep2-BinA_AmpliconSequencing"
],
"assay_term": {
"@id": "/assay-terms/OBI_0003661/",
"status": "released",
"term_name": "in vitro CRISPR screen using flow cytometry"
},
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Released in vitro system [IGVFSM9903HPOM](/in-vitro-systems/IGVFSM9903HPOM/) has in progress subobject construct library set [IGVFDS2385DHZO](/construct-library-sets/IGVFDS2385DHZO/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/in-vitro-systems/IGVFSM9903HPOM/"
},
{
"category": "missing sequencing kit",
"detail": "Measurement set [IGVFDS5594RPYL](/measurement-sets/IGVFDS5594RPYL/) has sequence file(s) [IGVFFI9231PIPG](/sequence-files/IGVFFI9231PIPG/), [IGVFFI2681IIGL](/sequence-files/IGVFFI2681IIGL/), [IGVFFI8689ABQR](/sequence-files/IGVFFI8689ABQR/), [IGVFFI7718EVQL](/sequence-files/IGVFFI7718EVQL/), [IGVFFI8500CMAV](/sequence-files/IGVFFI8500CMAV/), [IGVFFI1054FCNZ](/sequence-files/IGVFFI1054FCNZ/), [IGVFFI0031JMAT](/sequence-files/IGVFFI0031JMAT/), [IGVFFI2858OLQC](/sequence-files/IGVFFI2858OLQC/), [IGVFFI0485EDMQ](/sequence-files/IGVFFI0485EDMQ/), [IGVFFI3951OTQY](/sequence-files/IGVFFI3951OTQY/), [IGVFFI8338URBN](/sequence-files/IGVFFI8338URBN/), [IGVFFI6546KSCF](/sequence-files/IGVFFI6546KSCF/), [IGVFFI9384MWPH](/sequence-files/IGVFFI9384MWPH/), [IGVFFI9545RNNK](/sequence-files/IGVFFI9545RNNK/), [IGVFFI5841VHFF](/sequence-files/IGVFFI5841VHFF/), [IGVFFI0171PGWV](/sequence-files/IGVFFI0171PGWV/), [IGVFFI7973SEPY](/sequence-files/IGVFFI7973SEPY/), [IGVFFI0580ZTKR](/sequence-files/IGVFFI0580ZTKR/), [IGVFFI6282LBFO](/sequence-files/IGVFFI6282LBFO/), [IGVFFI9370KLSB](/sequence-files/IGVFFI9370KLSB/), [IGVFFI5244EAET](/sequence-files/IGVFFI5244EAET/), [IGVFFI2324TABT](/sequence-files/IGVFFI2324TABT/), [IGVFFI0389JZVT](/sequence-files/IGVFFI0389JZVT/), [IGVFFI8050WJCF](/sequence-files/IGVFFI8050WJCF/), [IGVFFI8758VZXX](/sequence-files/IGVFFI8758VZXX/), [IGVFFI8583NSCO](/sequence-files/IGVFFI8583NSCO/), [IGVFFI0408KCIO](/sequence-files/IGVFFI0408KCIO/), [IGVFFI8562MAQU](/sequence-files/IGVFFI8562MAQU/), [IGVFFI9078FCBA](/sequence-files/IGVFFI9078FCBA/), [IGVFFI8071HHVF](/sequence-files/IGVFFI8071HHVF/), [IGVFFI4286JSLT](/sequence-files/IGVFFI4286JSLT/), [IGVFFI2114YKHG](/sequence-files/IGVFFI2114YKHG/), [IGVFFI2153OCDA](/sequence-files/IGVFFI2153OCDA/), [IGVFFI8979HRIF](/sequence-files/IGVFFI8979HRIF/), [IGVFFI4060NZKT](/sequence-files/IGVFFI4060NZKT/), [IGVFFI9029NKIB](/sequence-files/IGVFFI9029NKIB/), [IGVFFI0803RUVZ](/sequence-files/IGVFFI0803RUVZ/), [IGVFFI7283EAPW](/sequence-files/IGVFFI7283EAPW/), [IGVFFI3600TPJQ](/sequence-files/IGVFFI3600TPJQ/), [IGVFFI2552YCGQ](/sequence-files/IGVFFI2552YCGQ/), [IGVFFI6915HJTC](/sequence-files/IGVFFI6915HJTC/), [IGVFFI0222WGQD](/sequence-files/IGVFFI0222WGQD/), [IGVFFI0050DNPN](/sequence-files/IGVFFI0050DNPN/), [IGVFFI8157MRLJ](/sequence-files/IGVFFI8157MRLJ/), [IGVFFI5798TJGY](/sequence-files/IGVFFI5798TJGY/), [IGVFFI1502OXFK](/sequence-files/IGVFFI1502OXFK/), [IGVFFI4246MURX](/sequence-files/IGVFFI4246MURX/), [IGVFFI1598RDSQ](/sequence-files/IGVFFI1598RDSQ/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_inconsistent_sequencing_kit",
"path": "/measurement-sets/IGVFDS5594RPYL/"
},
{
"category": "missing sequence specification",
"detail": "Measurement set [IGVFDS5594RPYL](/measurement-sets/IGVFDS5594RPYL/) has sequence file(s): [IGVFFI9231PIPG](/sequence-files/IGVFFI9231PIPG/), [IGVFFI2681IIGL](/sequence-files/IGVFFI2681IIGL/), [IGVFFI8689ABQR](/sequence-files/IGVFFI8689ABQR/), [IGVFFI7718EVQL](/sequence-files/IGVFFI7718EVQL/), [IGVFFI8500CMAV](/sequence-files/IGVFFI8500CMAV/), [IGVFFI1054FCNZ](/sequence-files/IGVFFI1054FCNZ/), [IGVFFI0031JMAT](/sequence-files/IGVFFI0031JMAT/), [IGVFFI2858OLQC](/sequence-files/IGVFFI2858OLQC/), [IGVFFI0485EDMQ](/sequence-files/IGVFFI0485EDMQ/), [IGVFFI3951OTQY](/sequence-files/IGVFFI3951OTQY/), [IGVFFI8338URBN](/sequence-files/IGVFFI8338URBN/), [IGVFFI6546KSCF](/sequence-files/IGVFFI6546KSCF/), [IGVFFI9384MWPH](/sequence-files/IGVFFI9384MWPH/), [IGVFFI9545RNNK](/sequence-files/IGVFFI9545RNNK/), [IGVFFI5841VHFF](/sequence-files/IGVFFI5841VHFF/), [IGVFFI0171PGWV](/sequence-files/IGVFFI0171PGWV/), [IGVFFI7973SEPY](/sequence-files/IGVFFI7973SEPY/), [IGVFFI0580ZTKR](/sequence-files/IGVFFI0580ZTKR/), [IGVFFI6282LBFO](/sequence-files/IGVFFI6282LBFO/), [IGVFFI9370KLSB](/sequence-files/IGVFFI9370KLSB/), [IGVFFI5244EAET](/sequence-files/IGVFFI5244EAET/), [IGVFFI2324TABT](/sequence-files/IGVFFI2324TABT/), [IGVFFI0389JZVT](/sequence-files/IGVFFI0389JZVT/), [IGVFFI8050WJCF](/sequence-files/IGVFFI8050WJCF/), [IGVFFI8758VZXX](/sequence-files/IGVFFI8758VZXX/), [IGVFFI8583NSCO](/sequence-files/IGVFFI8583NSCO/), [IGVFFI0408KCIO](/sequence-files/IGVFFI0408KCIO/), [IGVFFI8562MAQU](/sequence-files/IGVFFI8562MAQU/), [IGVFFI9078FCBA](/sequence-files/IGVFFI9078FCBA/), [IGVFFI8071HHVF](/sequence-files/IGVFFI8071HHVF/), [IGVFFI4286JSLT](/sequence-files/IGVFFI4286JSLT/), [IGVFFI2114YKHG](/sequence-files/IGVFFI2114YKHG/), [IGVFFI2153OCDA](/sequence-files/IGVFFI2153OCDA/), [IGVFFI8979HRIF](/sequence-files/IGVFFI8979HRIF/), [IGVFFI4060NZKT](/sequence-files/IGVFFI4060NZKT/), [IGVFFI9029NKIB](/sequence-files/IGVFFI9029NKIB/), [IGVFFI0803RUVZ](/sequence-files/IGVFFI0803RUVZ/), [IGVFFI7283EAPW](/sequence-files/IGVFFI7283EAPW/), [IGVFFI3600TPJQ](/sequence-files/IGVFFI3600TPJQ/), [IGVFFI2552YCGQ](/sequence-files/IGVFFI2552YCGQ/), [IGVFFI6915HJTC](/sequence-files/IGVFFI6915HJTC/), [IGVFFI0222WGQD](/sequence-files/IGVFFI0222WGQD/), [IGVFFI0050DNPN](/sequence-files/IGVFFI0050DNPN/), [IGVFFI8157MRLJ](/sequence-files/IGVFFI8157MRLJ/), [IGVFFI5798TJGY](/sequence-files/IGVFFI5798TJGY/), [IGVFFI1502OXFK](/sequence-files/IGVFFI1502OXFK/), [IGVFFI4246MURX](/sequence-files/IGVFFI4246MURX/), [IGVFFI1598RDSQ](/sequence-files/IGVFFI1598RDSQ/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_missing_seqspec",
"path": "/measurement-sets/IGVFDS5594RPYL/"
}
],
"WARNING": [
{
"category": "missing auxiliary set",
"detail": "Measurement set [IGVFDS5594RPYL](/measurement-sets/IGVFDS5594RPYL/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
"level": 40,
"level_name": "WARNING",
"name": "audit_missing_auxiliary_set",
"path": "/measurement-sets/IGVFDS5594RPYL/"
}
]
},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
"title": "Jesse Engreitz"
},
"title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
},
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{
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"accession": "IGVFDS4869FXYY",
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],
"status": "released"
}
],
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"creation_timestamp": "2023-12-13T08:48:11.548049+00:00",
"donors": [
{
"@id": "/human-donors/IGVFDO1080XFGV/",
"accession": "IGVFDO1080XFGV",
"aliases": [
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],
"sex": "male",
"status": "released",
"taxa": "Homo sapiens"
}
],
"externally_hosted": false,
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},
"submitted_files_timestamp": "2023-12-13T10:08:21.776414+00:00",
"summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
"targeted_genes": [
{
"@id": "/genes/ENSG00000108179/",
"geneid": "ENSG00000108179",
"name": "peptidylprolyl isomerase F",
"status": "released",
"symbol": "PPIF",
"synonyms": [
"rotamase F",
"CYP3",
"cyclophilin F",
"mitochondrial cyclophilin",
"peptidyl-prolyl cis-trans isomerase, mitochondrial",
"CypD",
"Cyp-D",
"cyclophilin 3",
"cyclophilin D",
"CyP-M",
"peptidyl-prolyl cis-trans isomerase F, mitochondrial",
"PPIase F"
]
}
],
"uuid": "c8166490-fcfe-43b9-a229-122af3a37dc8"
}