IGVFDS5572SQRK

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

CCND1

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Construct Library Sets

IGVFDS6351FGNG guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF1-BinB-PCR1-Rep1_S242

jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF1-BinB-PCR2-Rep1_S248

jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF1-BinB-PCR3-Rep1_S284

jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF1-BinB-PCR4-Rep1_S290

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM5255ABUW

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS0051MRCH

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI4348ZUXP, IGVFFI0056ZWFB, IGVFFI1781YSJE, IGVFFI8211KHIX

jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin6hrs-FF1-BinB-PCR1-Rep1_S242
jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin6hrs-FF1-BinB-PCR2-Rep1_S248
jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin6hrs-FF1-BinB-PCR3-Rep1_S284
jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin6hrs-FF1-BinB-PCR4-Rep1_S290

Jesse Engreitz, Stanford

IGVFDS6842FAIJ

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI5841PKJL