IGVFDS5560HMQA

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

MYC

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Construct Library Sets

IGVFDS7857SYCK guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR1_Rep1_S2

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR1_Rep2_S57

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR2_Rep1_S8

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR2_Rep2_S63

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR3_Rep1_S14

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR3_Rep2_S69

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR4_Rep1_S20

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR4_Rep2_S75

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM3954RGXF

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS4701YCEL

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI6952RHQM, IGVFFI1122HKWU, IGVFFI1697DZHB, IGVFFI8224NGZQ, IGVFFI7565VCLO, IGVFFI3742WWYT, IGVFFI7880EKFD, IGVFFI1267BFAZ

jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR1_Rep1_S2
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR1_Rep2_S57
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR2_Rep1_S8
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR2_Rep2_S63
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR3_Rep1_S14
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR3_Rep2_S69
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR4_Rep1_S20
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR4_Rep2_S75

Jesse Engreitz, Stanford

IGVFDS5951VSHM

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI2212HXUF