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IGVFDS5251YUKR

released
File Set Type
experimental data
Summary
CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Assay Term
in vitro CRISPR screen using flow cytometry
Targeted Genes
Sample Summaries
  • HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
Construct Library Sets
  • IGVFDS5413RKIA Guide (sgRNA) library targeting accessible genome regions in many genomic loci
Protocols
Aliases
jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinD-PCR1-Rep1_S16
jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinD-PCR2-Rep1_S22
jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinD-PCR3-Rep1_S70
jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinD-PCR4-Rep1_S76

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford