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INDEXED
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MeasurementSet
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IGVFDS5211BLJX
IGVFDS5211BLJX
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File Set Type
experimental data
Summary
CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3
Assay Term
in vitro CRISPR screen using flow cytometry
Sample Summaries
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin D for 76-100% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
Construct Library Sets
IGVFDS8953DFJK
Guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3
Protocols
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
Aliases
jesse-engreitz:measurement_NOS3_CRISPRi_FF_R2_FFrep2_binD_PCR2_S220
Samples
Donors
Auxiliary Datasets
Documents
Attribution
Award
HG011972
Principal Investigator(s)
Anshul Kundaje
,
Jesse Engreitz
,
Marlene Rabinovitch
,
Lars Steinmetz
,
Michael Bassik
,
Thomas Quertermous
,
Will Greenleaf
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford