IGVFDS4992SJIR

released
{
    "@context": "/terms/",
    "@id": "/measurement-sets/IGVFDS4992SJIR/",
    "@type": [
        "MeasurementSet",
        "FileSet",
        "Item"
    ],
    "accession": "IGVFDS4992SJIR",
    "aliases": [
        "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep1-FFrep1-BinC_AmpliconSequencing"
    ],
    "assay_term": {
        "@id": "/assay-terms/OBI_0003661/",
        "status": "released",
        "term_name": "in vitro CRISPR screen using flow cytometry"
    },
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "mismatched status",
                "detail": "Released in vitro system [IGVFSM0211VADB](/in-vitro-systems/IGVFSM0211VADB/) has in progress subobject construct library set [IGVFDS2385DHZO](/construct-library-sets/IGVFDS2385DHZO/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/in-vitro-systems/IGVFSM0211VADB/"
            },
            {
                "category": "missing sequencing kit",
                "detail": "Measurement set [IGVFDS4992SJIR](/measurement-sets/IGVFDS4992SJIR/) has sequence file(s) [IGVFFI5549CHLU](/sequence-files/IGVFFI5549CHLU/), [IGVFFI0562OYGY](/sequence-files/IGVFFI0562OYGY/), [IGVFFI2423UWAD](/sequence-files/IGVFFI2423UWAD/), [IGVFFI0599ZVIO](/sequence-files/IGVFFI0599ZVIO/), [IGVFFI6684FFVA](/sequence-files/IGVFFI6684FFVA/), [IGVFFI8356EFPN](/sequence-files/IGVFFI8356EFPN/), [IGVFFI3871RFWM](/sequence-files/IGVFFI3871RFWM/), [IGVFFI8254TGUJ](/sequence-files/IGVFFI8254TGUJ/), [IGVFFI3899MXNI](/sequence-files/IGVFFI3899MXNI/), [IGVFFI9936TKIK](/sequence-files/IGVFFI9936TKIK/), [IGVFFI4943BGIB](/sequence-files/IGVFFI4943BGIB/), [IGVFFI8355IDSV](/sequence-files/IGVFFI8355IDSV/), [IGVFFI4161SIGX](/sequence-files/IGVFFI4161SIGX/), [IGVFFI2718UTCH](/sequence-files/IGVFFI2718UTCH/), [IGVFFI9345FNGM](/sequence-files/IGVFFI9345FNGM/), [IGVFFI3329HTMU](/sequence-files/IGVFFI3329HTMU/), [IGVFFI8900WQQK](/sequence-files/IGVFFI8900WQQK/), [IGVFFI0375AEDO](/sequence-files/IGVFFI0375AEDO/), [IGVFFI9278TQFP](/sequence-files/IGVFFI9278TQFP/), [IGVFFI9483VTQL](/sequence-files/IGVFFI9483VTQL/), [IGVFFI7303RRJL](/sequence-files/IGVFFI7303RRJL/), [IGVFFI0260HBHT](/sequence-files/IGVFFI0260HBHT/), [IGVFFI4801NKXE](/sequence-files/IGVFFI4801NKXE/), [IGVFFI2949RROQ](/sequence-files/IGVFFI2949RROQ/), [IGVFFI3677HQDU](/sequence-files/IGVFFI3677HQDU/), [IGVFFI9629BMAF](/sequence-files/IGVFFI9629BMAF/), [IGVFFI9685IBBO](/sequence-files/IGVFFI9685IBBO/), [IGVFFI1357VFYV](/sequence-files/IGVFFI1357VFYV/), [IGVFFI9423TIVC](/sequence-files/IGVFFI9423TIVC/), [IGVFFI7936BICU](/sequence-files/IGVFFI7936BICU/), [IGVFFI9553QXYB](/sequence-files/IGVFFI9553QXYB/), [IGVFFI4130VHAP](/sequence-files/IGVFFI4130VHAP/), [IGVFFI1256JMOD](/sequence-files/IGVFFI1256JMOD/), [IGVFFI3806NOME](/sequence-files/IGVFFI3806NOME/), [IGVFFI5801PIVF](/sequence-files/IGVFFI5801PIVF/), [IGVFFI6011RQXY](/sequence-files/IGVFFI6011RQXY/), [IGVFFI2754CINX](/sequence-files/IGVFFI2754CINX/), [IGVFFI6940ATQU](/sequence-files/IGVFFI6940ATQU/), [IGVFFI5875TDDK](/sequence-files/IGVFFI5875TDDK/), [IGVFFI6819BIOR](/sequence-files/IGVFFI6819BIOR/), [IGVFFI3226VHAN](/sequence-files/IGVFFI3226VHAN/), [IGVFFI6370MJCP](/sequence-files/IGVFFI6370MJCP/), [IGVFFI7880RQBS](/sequence-files/IGVFFI7880RQBS/), [IGVFFI9951PDDP](/sequence-files/IGVFFI9951PDDP/), [IGVFFI0914CAEI](/sequence-files/IGVFFI0914CAEI/), [IGVFFI0471UPCF](/sequence-files/IGVFFI0471UPCF/), [IGVFFI6561AUAU](/sequence-files/IGVFFI6561AUAU/), [IGVFFI1161GFXT](/sequence-files/IGVFFI1161GFXT/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/measurement-sets/IGVFDS4992SJIR/"
            },
            {
                "category": "missing sequence specification",
                "detail": "Measurement set [IGVFDS4992SJIR](/measurement-sets/IGVFDS4992SJIR/) has sequence file(s): [IGVFFI5549CHLU](/sequence-files/IGVFFI5549CHLU/), [IGVFFI0562OYGY](/sequence-files/IGVFFI0562OYGY/), [IGVFFI2423UWAD](/sequence-files/IGVFFI2423UWAD/), [IGVFFI0599ZVIO](/sequence-files/IGVFFI0599ZVIO/), [IGVFFI6684FFVA](/sequence-files/IGVFFI6684FFVA/), [IGVFFI8356EFPN](/sequence-files/IGVFFI8356EFPN/), [IGVFFI3871RFWM](/sequence-files/IGVFFI3871RFWM/), [IGVFFI8254TGUJ](/sequence-files/IGVFFI8254TGUJ/), [IGVFFI3899MXNI](/sequence-files/IGVFFI3899MXNI/), [IGVFFI9936TKIK](/sequence-files/IGVFFI9936TKIK/), [IGVFFI4943BGIB](/sequence-files/IGVFFI4943BGIB/), [IGVFFI8355IDSV](/sequence-files/IGVFFI8355IDSV/), [IGVFFI4161SIGX](/sequence-files/IGVFFI4161SIGX/), [IGVFFI2718UTCH](/sequence-files/IGVFFI2718UTCH/), [IGVFFI9345FNGM](/sequence-files/IGVFFI9345FNGM/), [IGVFFI3329HTMU](/sequence-files/IGVFFI3329HTMU/), [IGVFFI8900WQQK](/sequence-files/IGVFFI8900WQQK/), [IGVFFI0375AEDO](/sequence-files/IGVFFI0375AEDO/), [IGVFFI9278TQFP](/sequence-files/IGVFFI9278TQFP/), [IGVFFI9483VTQL](/sequence-files/IGVFFI9483VTQL/), [IGVFFI7303RRJL](/sequence-files/IGVFFI7303RRJL/), [IGVFFI0260HBHT](/sequence-files/IGVFFI0260HBHT/), [IGVFFI4801NKXE](/sequence-files/IGVFFI4801NKXE/), [IGVFFI2949RROQ](/sequence-files/IGVFFI2949RROQ/), [IGVFFI3677HQDU](/sequence-files/IGVFFI3677HQDU/), [IGVFFI9629BMAF](/sequence-files/IGVFFI9629BMAF/), [IGVFFI9685IBBO](/sequence-files/IGVFFI9685IBBO/), [IGVFFI1357VFYV](/sequence-files/IGVFFI1357VFYV/), [IGVFFI9423TIVC](/sequence-files/IGVFFI9423TIVC/), [IGVFFI7936BICU](/sequence-files/IGVFFI7936BICU/), [IGVFFI9553QXYB](/sequence-files/IGVFFI9553QXYB/), [IGVFFI4130VHAP](/sequence-files/IGVFFI4130VHAP/), [IGVFFI1256JMOD](/sequence-files/IGVFFI1256JMOD/), [IGVFFI3806NOME](/sequence-files/IGVFFI3806NOME/), [IGVFFI5801PIVF](/sequence-files/IGVFFI5801PIVF/), [IGVFFI6011RQXY](/sequence-files/IGVFFI6011RQXY/), [IGVFFI2754CINX](/sequence-files/IGVFFI2754CINX/), [IGVFFI6940ATQU](/sequence-files/IGVFFI6940ATQU/), [IGVFFI5875TDDK](/sequence-files/IGVFFI5875TDDK/), [IGVFFI6819BIOR](/sequence-files/IGVFFI6819BIOR/), [IGVFFI3226VHAN](/sequence-files/IGVFFI3226VHAN/), [IGVFFI6370MJCP](/sequence-files/IGVFFI6370MJCP/), [IGVFFI7880RQBS](/sequence-files/IGVFFI7880RQBS/), [IGVFFI9951PDDP](/sequence-files/IGVFFI9951PDDP/), [IGVFFI0914CAEI](/sequence-files/IGVFFI0914CAEI/), [IGVFFI0471UPCF](/sequence-files/IGVFFI0471UPCF/), [IGVFFI6561AUAU](/sequence-files/IGVFFI6561AUAU/), [IGVFFI1161GFXT](/sequence-files/IGVFFI1161GFXT/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_missing_seqspec",
                "path": "/measurement-sets/IGVFDS4992SJIR/"
            }
        ],
        "WARNING": [
            {
                "category": "missing auxiliary set",
                "detail": "Measurement set [IGVFDS4992SJIR](/measurement-sets/IGVFDS4992SJIR/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_missing_auxiliary_set",
                "path": "/measurement-sets/IGVFDS4992SJIR/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization",
        "contact_pi": {
            "@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
            "title": "Jesse Engreitz"
        },
        "title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
    },
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            "@id": "/measurement-sets/IGVFDS4869FXYY/",
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            "aliases": [
                "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-Jurkat-PE2_Exp2_NegControl_AmpliconSequencing"
            ],
            "status": "released"
        }
    ],
    "control_for": [],
    "creation_timestamp": "2023-12-13T08:48:03.335945+00:00",
    "donors": [
        {
            "@id": "/human-donors/IGVFDO1080XFGV/",
            "accession": "IGVFDO1080XFGV",
            "aliases": [
                "igvf:donor_of_Jurkat"
            ],
            "sex": "male",
            "status": "released",
            "taxa": "Homo sapiens"
        }
    ],
    "externally_hosted": false,
    "file_set_type": "experimental data",
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        },
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            "@id": "/sequence-files/IGVFFI4943BGIB/",
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            "sequencing_platform": {
                "@id": "/platform-terms/EFO_0008566/",
                "status": "released",
                "term_name": "Illumina NextSeq 550"
            },
            "status": "released",
            "submitted_file_name": "230423pPPIFEnformerJurkat-Jurkat-PE-pPPIF_Enformer-Amp-pPPIF-BioRep1-FFRep1_BinC-PCRrep8_S32_R1_001.fastq.gz",
            "upload_status": "validated"
        }
    ],
    "input_for": [
        "/analysis-sets/IGVFDS6299IJHG/"
    ],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set. This measurement set previously used Variant FlowFISH as a preferred_assay_title, but this enum is now removed. So it has been defaulted to Variant-EFFECTS.",
    "preferred_assay_title": "Variant-EFFECTS",
    "protocols": [
        "https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
    ],
    "release_timestamp": "2023-12-20T23:08:25.885432+00:00",
    "samples": [
        {
            "@id": "/in-vitro-systems/IGVFSM0211VADB/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM0211VADB",
            "aliases": [
                "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep1-FFrep1-BinC"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                {
                    "@id": "/construct-library-sets/IGVFDS2385DHZO/",
                    "accession": "IGVFDS2385DHZO",
                    "file_set_type": "guide library",
                    "status": "in progress",
                    "summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
                }
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0002796/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:Jurkat"
                    ],
                    "status": "released",
                    "summary": "Jurkat",
                    "term_name": "Jurkat"
                }
            ],
            "status": "released",
            "summary": "Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        }
    ],
    "schema_version": "23",
    "status": "released",
    "submitted_by": {
        "@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
        "title": "Michael Montgomery"
    },
    "submitted_files_timestamp": "2023-12-13T09:19:29.851970+00:00",
    "summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
    "targeted_genes": [
        {
            "@id": "/genes/ENSG00000108179/",
            "geneid": "ENSG00000108179",
            "name": "peptidylprolyl isomerase F",
            "status": "released",
            "symbol": "PPIF",
            "synonyms": [
                "rotamase F",
                "CYP3",
                "cyclophilin F",
                "mitochondrial cyclophilin",
                "peptidyl-prolyl cis-trans isomerase, mitochondrial",
                "CypD",
                "Cyp-D",
                "cyclophilin 3",
                "cyclophilin D",
                "CyP-M",
                "peptidyl-prolyl cis-trans isomerase F, mitochondrial",
                "PPIase F"
            ]
        }
    ],
    "uuid": "2d9fe6f0-79d5-4518-9c3d-6e580ddafa3e"
}