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MeasurementSet
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IGVFDS4759EOBT
IGVFDS4759EOBT
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This object is in preview status. Metadata is subject to change until this object is released.
File Set Type
experimental data
Summary
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci
Assay Term
in vitro CRISPR screen using single-cell RNA-seq
Sample Summaries
Homo sapiens (female) TeloHAEC cell line modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
Protocols
https://www.protocols.io/view/systematic-perturb-seq-to-discover-endothelial-cel-261ged73yv47/v1
Aliases
jesse-engreitz:measurement_set-scRNAseq_2kG_2PMDox2_3
Publications
Convergence of coronary artery disease genes onto endothelial cell programs
Attribution
Jesse Engreitz, Stanford
(
HG011972
—
Jesse Engreitz
)
Sequencing Results (Illumina)
15 items
1
2
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Read Names
Associated seqspec Files
Sequence Specification Document
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI0134XOMG
fastq
reads
R1
3
Illumina NovaSeq 6000
4.1 GB
Jesse Engreitz, Stanford
validated
IGVFFI1000OYZT
fastq
reads
R2
3
Illumina NovaSeq 6000
5.8 GB
Jesse Engreitz, Stanford
validated
IGVFFI1348RZIY
fastq
reads
R2
1
Illumina NovaSeq 6000
5.3 GB
Jesse Engreitz, Stanford
validated
IGVFFI3249CZPL
fastq
reads
R1
2
Illumina NovaSeq 6000
3.6 GB
Jesse Engreitz, Stanford
validated
IGVFFI4127XHHO
fastq
reads
I1
5
Illumina NovaSeq 6000
2.9 GB
Jesse Engreitz, Stanford
validated
IGVFFI4710IMRW
fastq
reads
I1
1
Illumina NovaSeq 6000
1.1 GB
Jesse Engreitz, Stanford
validated
IGVFFI4995FGLZ
fastq
reads
I1
3
Illumina NovaSeq 6000
1.1 GB
Jesse Engreitz, Stanford
validated
IGVFFI5046KDTH
fastq
reads
R1
4
Illumina NovaSeq 6000
3.6 GB
Jesse Engreitz, Stanford
validated
IGVFFI6203SWWL
fastq
reads
R2
5
Illumina NovaSeq 6000
14 GB
Jesse Engreitz, Stanford
validated
IGVFFI6584LMAN
fastq
reads
I1
2
Illumina NovaSeq 6000
1.0 GB
Jesse Engreitz, Stanford
validated
Samples
1 item
Accession
Type
Sample Terms
Associated Construct Library Sets
Summary
IGVFSM0914ERRM
InVitroSystem
TeloHAEC
IGVFDS1298TUYG
Homo sapiens (female) TeloHAEC cell line modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO3459DFTW
igvf:donor_of_teloHAEC
female
Homo sapiens
Associated Construct Library Sets
1 item
Accession
Summary
Library Design Files
IGVFDS1298TUYG
guide (sgRNA) library targeting genes in many genomic loci
IGVFFI9754AGFB
File Sets Using This Measurement Set as an Input
1 item
Accession
Type
Summary
Aliases
Lab
IGVFDS7943IIWZ
AnalysisSet
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci
jesse-engreitz:analysis_set_TeloHAEC_Perturb-seq_2kG
Jesse Engreitz, Stanford
Auxiliary Datasets
1 item
Accession
Type
Summary
Files
Aliases
Lab
IGVFDS4972ICEM
AuxiliarySet
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci
IGVFFI5615OENT
,
IGVFFI8376NVAX
,
IGVFFI9779WRBT
,
IGVFFI9575YXQY
jesse-engreitz:auxiliary_sets-scRNAseq_2kG_2PMDox2_3
Jesse Engreitz, Stanford