IGVFDS4759EOBT

preview

This object is in preview status. Metadata is subject to change until this object is released.

File Set Type

experimental data

Summary

CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci

Assay Term

in vitro CRISPR screen using single-cell RNA-seq

Sample Summaries

Homo sapiens (female) TeloHAEC cell line modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library

Protocols

https://www.protocols.io/view/systematic-perturb-seq-to-discover-endothelial-cel-261ged73yv47/v1

Aliases

jesse-engreitz:measurement_set-scRNAseq_2kG_2PMDox2_3

Publications

Convergence of coronary artery disease genes onto endothelial cell programs

Attribution

Jesse Engreitz, Stanford

(

HG011972 — Jesse Engreitz

)

Sequencing Results (Illumina)

15 items

Read Names

IGVFFI0134XOMG

fastq

reads

Illumina NovaSeq 6000

4.1 GB

Jesse Engreitz, Stanford

validated

IGVFFI1000OYZT

fastq

reads

Illumina NovaSeq 6000

5.8 GB

Jesse Engreitz, Stanford

validated

IGVFFI1348RZIY

fastq

reads

Illumina NovaSeq 6000

5.3 GB

Jesse Engreitz, Stanford

validated

IGVFFI3249CZPL

fastq

reads

Illumina NovaSeq 6000

3.6 GB

Jesse Engreitz, Stanford

validated

IGVFFI4127XHHO

fastq

reads

Illumina NovaSeq 6000

2.9 GB

Jesse Engreitz, Stanford

validated

IGVFFI4710IMRW

fastq

reads

Illumina NovaSeq 6000

1.1 GB

Jesse Engreitz, Stanford

validated

IGVFFI4995FGLZ

fastq

reads

Illumina NovaSeq 6000

1.1 GB

Jesse Engreitz, Stanford

validated

IGVFFI5046KDTH

fastq

reads

Illumina NovaSeq 6000

3.6 GB

Jesse Engreitz, Stanford

validated

IGVFFI6203SWWL

fastq

reads

Illumina NovaSeq 6000

14 GB

Jesse Engreitz, Stanford

validated

IGVFFI6584LMAN

fastq

reads

Illumina NovaSeq 6000

1.0 GB

Jesse Engreitz, Stanford

validated

Samples

1 item

Accession

Type

Sample Terms

Associated Construct Library Sets

Summary

IGVFSM0914ERRM

InVitroSystem

TeloHAEC

IGVFDS1298TUYG

Homo sapiens (female) TeloHAEC cell line modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO3459DFTW

igvf:donor_of_teloHAEC

female

Homo sapiens

Associated Construct Library Sets

1 item

Accession

Summary

Library Design Files

IGVFDS1298TUYG

guide (sgRNA) library targeting genes in many genomic loci

IGVFFI9754AGFB

File Sets Using This Measurement Set as an Input

1 item

Accession

Type

Summary

Aliases

Lab

IGVFDS7943IIWZ

AnalysisSet

CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci

jesse-engreitz:analysis_set_TeloHAEC_Perturb-seq_2kG

Jesse Engreitz, Stanford

Auxiliary Datasets

1 item

Accession

Type

Summary

Files

Aliases

Lab

IGVFDS4972ICEM

AuxiliarySet

gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci

IGVFFI5615OENT, IGVFFI8376NVAX, IGVFFI9779WRBT, IGVFFI9575YXQY

jesse-engreitz:auxiliary_sets-scRNAseq_2kG_2PMDox2_3

Jesse Engreitz, Stanford