IGVFDS4752FLON

released
{
    "@context": "/terms/",
    "@id": "/measurement-sets/IGVFDS4752FLON/",
    "@type": [
        "MeasurementSet",
        "FileSet",
        "Item"
    ],
    "accession": "IGVFDS4752FLON",
    "aliases": [
        "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep1-FFrep3-BinB_AmpliconSequencing"
    ],
    "assay_term": {
        "@id": "/assay-terms/OBI_0003661/",
        "status": "released",
        "term_name": "in vitro CRISPR screen using flow cytometry"
    },
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "mismatched status",
                "detail": "Released in vitro system [IGVFSM1838USXI](/in-vitro-systems/IGVFSM1838USXI/) has in progress subobject construct library set [IGVFDS2385DHZO](/construct-library-sets/IGVFDS2385DHZO/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/in-vitro-systems/IGVFSM1838USXI/"
            },
            {
                "category": "missing sequencing kit",
                "detail": "Measurement set [IGVFDS4752FLON](/measurement-sets/IGVFDS4752FLON/) has sequence file(s) [IGVFFI4064LQPH](/sequence-files/IGVFFI4064LQPH/), [IGVFFI7666HMMV](/sequence-files/IGVFFI7666HMMV/), [IGVFFI9608CCLH](/sequence-files/IGVFFI9608CCLH/), [IGVFFI8562BHLG](/sequence-files/IGVFFI8562BHLG/), [IGVFFI3807DWRT](/sequence-files/IGVFFI3807DWRT/), [IGVFFI2362JMED](/sequence-files/IGVFFI2362JMED/), [IGVFFI9512QSXF](/sequence-files/IGVFFI9512QSXF/), [IGVFFI4106OCGW](/sequence-files/IGVFFI4106OCGW/), [IGVFFI4620UWTA](/sequence-files/IGVFFI4620UWTA/), [IGVFFI1749VWLS](/sequence-files/IGVFFI1749VWLS/), [IGVFFI8418DFET](/sequence-files/IGVFFI8418DFET/), [IGVFFI1665PBUX](/sequence-files/IGVFFI1665PBUX/), [IGVFFI2720CWNA](/sequence-files/IGVFFI2720CWNA/), [IGVFFI9058GJCO](/sequence-files/IGVFFI9058GJCO/), [IGVFFI8233CCAJ](/sequence-files/IGVFFI8233CCAJ/), [IGVFFI4315UCLC](/sequence-files/IGVFFI4315UCLC/), [IGVFFI9218RWCU](/sequence-files/IGVFFI9218RWCU/), [IGVFFI2966KTVV](/sequence-files/IGVFFI2966KTVV/), [IGVFFI2816IJOL](/sequence-files/IGVFFI2816IJOL/), [IGVFFI3061PCTG](/sequence-files/IGVFFI3061PCTG/), [IGVFFI2013KLCS](/sequence-files/IGVFFI2013KLCS/), [IGVFFI5457UYFE](/sequence-files/IGVFFI5457UYFE/), [IGVFFI5253KQNA](/sequence-files/IGVFFI5253KQNA/), [IGVFFI3560JRAD](/sequence-files/IGVFFI3560JRAD/), [IGVFFI4224TVST](/sequence-files/IGVFFI4224TVST/), [IGVFFI2889NFNT](/sequence-files/IGVFFI2889NFNT/), [IGVFFI4196IBSF](/sequence-files/IGVFFI4196IBSF/), [IGVFFI6885RUCL](/sequence-files/IGVFFI6885RUCL/), [IGVFFI3401FTPY](/sequence-files/IGVFFI3401FTPY/), [IGVFFI0601WFWF](/sequence-files/IGVFFI0601WFWF/), [IGVFFI0944CZSS](/sequence-files/IGVFFI0944CZSS/), [IGVFFI5454XMWM](/sequence-files/IGVFFI5454XMWM/), [IGVFFI4171NTKF](/sequence-files/IGVFFI4171NTKF/), [IGVFFI8614YIIT](/sequence-files/IGVFFI8614YIIT/), [IGVFFI9735OCYM](/sequence-files/IGVFFI9735OCYM/), [IGVFFI2673ZSJO](/sequence-files/IGVFFI2673ZSJO/), [IGVFFI3590RFFA](/sequence-files/IGVFFI3590RFFA/), [IGVFFI2309FCAH](/sequence-files/IGVFFI2309FCAH/), [IGVFFI1934NWYG](/sequence-files/IGVFFI1934NWYG/), [IGVFFI6681LQPA](/sequence-files/IGVFFI6681LQPA/), [IGVFFI6451VQAS](/sequence-files/IGVFFI6451VQAS/), [IGVFFI4406DBQR](/sequence-files/IGVFFI4406DBQR/), [IGVFFI2455DONN](/sequence-files/IGVFFI2455DONN/), [IGVFFI5663OEVS](/sequence-files/IGVFFI5663OEVS/), [IGVFFI8015VDZA](/sequence-files/IGVFFI8015VDZA/), [IGVFFI7546AOIT](/sequence-files/IGVFFI7546AOIT/), [IGVFFI6356SBUM](/sequence-files/IGVFFI6356SBUM/), [IGVFFI5911XZRL](/sequence-files/IGVFFI5911XZRL/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/measurement-sets/IGVFDS4752FLON/"
            },
            {
                "category": "missing sequence specification",
                "detail": "Measurement set [IGVFDS4752FLON](/measurement-sets/IGVFDS4752FLON/) has sequence file(s): [IGVFFI4064LQPH](/sequence-files/IGVFFI4064LQPH/), [IGVFFI7666HMMV](/sequence-files/IGVFFI7666HMMV/), [IGVFFI9608CCLH](/sequence-files/IGVFFI9608CCLH/), [IGVFFI8562BHLG](/sequence-files/IGVFFI8562BHLG/), [IGVFFI3807DWRT](/sequence-files/IGVFFI3807DWRT/), [IGVFFI2362JMED](/sequence-files/IGVFFI2362JMED/), [IGVFFI9512QSXF](/sequence-files/IGVFFI9512QSXF/), [IGVFFI4106OCGW](/sequence-files/IGVFFI4106OCGW/), [IGVFFI4620UWTA](/sequence-files/IGVFFI4620UWTA/), [IGVFFI1749VWLS](/sequence-files/IGVFFI1749VWLS/), [IGVFFI8418DFET](/sequence-files/IGVFFI8418DFET/), [IGVFFI1665PBUX](/sequence-files/IGVFFI1665PBUX/), [IGVFFI2720CWNA](/sequence-files/IGVFFI2720CWNA/), [IGVFFI9058GJCO](/sequence-files/IGVFFI9058GJCO/), [IGVFFI8233CCAJ](/sequence-files/IGVFFI8233CCAJ/), [IGVFFI4315UCLC](/sequence-files/IGVFFI4315UCLC/), [IGVFFI9218RWCU](/sequence-files/IGVFFI9218RWCU/), [IGVFFI2966KTVV](/sequence-files/IGVFFI2966KTVV/), [IGVFFI2816IJOL](/sequence-files/IGVFFI2816IJOL/), [IGVFFI3061PCTG](/sequence-files/IGVFFI3061PCTG/), [IGVFFI2013KLCS](/sequence-files/IGVFFI2013KLCS/), [IGVFFI5457UYFE](/sequence-files/IGVFFI5457UYFE/), [IGVFFI5253KQNA](/sequence-files/IGVFFI5253KQNA/), [IGVFFI3560JRAD](/sequence-files/IGVFFI3560JRAD/), [IGVFFI4224TVST](/sequence-files/IGVFFI4224TVST/), [IGVFFI2889NFNT](/sequence-files/IGVFFI2889NFNT/), [IGVFFI4196IBSF](/sequence-files/IGVFFI4196IBSF/), [IGVFFI6885RUCL](/sequence-files/IGVFFI6885RUCL/), [IGVFFI3401FTPY](/sequence-files/IGVFFI3401FTPY/), [IGVFFI0601WFWF](/sequence-files/IGVFFI0601WFWF/), [IGVFFI0944CZSS](/sequence-files/IGVFFI0944CZSS/), [IGVFFI5454XMWM](/sequence-files/IGVFFI5454XMWM/), [IGVFFI4171NTKF](/sequence-files/IGVFFI4171NTKF/), [IGVFFI8614YIIT](/sequence-files/IGVFFI8614YIIT/), [IGVFFI9735OCYM](/sequence-files/IGVFFI9735OCYM/), [IGVFFI2673ZSJO](/sequence-files/IGVFFI2673ZSJO/), [IGVFFI3590RFFA](/sequence-files/IGVFFI3590RFFA/), [IGVFFI2309FCAH](/sequence-files/IGVFFI2309FCAH/), [IGVFFI1934NWYG](/sequence-files/IGVFFI1934NWYG/), [IGVFFI6681LQPA](/sequence-files/IGVFFI6681LQPA/), [IGVFFI6451VQAS](/sequence-files/IGVFFI6451VQAS/), [IGVFFI4406DBQR](/sequence-files/IGVFFI4406DBQR/), [IGVFFI2455DONN](/sequence-files/IGVFFI2455DONN/), [IGVFFI5663OEVS](/sequence-files/IGVFFI5663OEVS/), [IGVFFI8015VDZA](/sequence-files/IGVFFI8015VDZA/), [IGVFFI7546AOIT](/sequence-files/IGVFFI7546AOIT/), [IGVFFI6356SBUM](/sequence-files/IGVFFI6356SBUM/), [IGVFFI5911XZRL](/sequence-files/IGVFFI5911XZRL/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_missing_seqspec",
                "path": "/measurement-sets/IGVFDS4752FLON/"
            }
        ],
        "WARNING": [
            {
                "category": "missing auxiliary set",
                "detail": "Measurement set [IGVFDS4752FLON](/measurement-sets/IGVFDS4752FLON/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_missing_auxiliary_set",
                "path": "/measurement-sets/IGVFDS4752FLON/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization",
        "contact_pi": {
            "@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
            "title": "Jesse Engreitz"
        },
        "title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
    },
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            "@id": "/measurement-sets/IGVFDS4869FXYY/",
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            "aliases": [
                "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-Jurkat-PE2_Exp2_NegControl_AmpliconSequencing"
            ],
            "status": "released"
        }
    ],
    "control_for": [],
    "creation_timestamp": "2023-12-13T08:48:06.644925+00:00",
    "donors": [
        {
            "@id": "/human-donors/IGVFDO1080XFGV/",
            "accession": "IGVFDO1080XFGV",
            "aliases": [
                "igvf:donor_of_Jurkat"
            ],
            "sex": "male",
            "status": "released",
            "taxa": "Homo sapiens"
        }
    ],
    "externally_hosted": false,
    "file_set_type": "experimental data",
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        },
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            "@id": "/sequence-files/IGVFFI8418DFET/",
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            "s3_uri": "s3://igvf-files/2023/12/13/96d8417a-4e4f-45d0-8820-7e70caa0c804/IGVFFI5911XZRL.fastq.gz",
            "sequencing_platform": {
                "@id": "/platform-terms/EFO_0008566/",
                "status": "released",
                "term_name": "Illumina NextSeq 550"
            },
            "status": "released",
            "submitted_file_name": "230423pPPIFEnformerJurkat-Jurkat-PE-pPPIF_Enformer-Amp-pPPIF-BioRep1-FFRep3_BinB-PCRrep4_S112_I2_001.fastq.gz",
            "upload_status": "validated"
        }
    ],
    "input_for": [
        "/analysis-sets/IGVFDS6299IJHG/"
    ],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set. This measurement set previously used Variant FlowFISH as a preferred_assay_title, but this enum is now removed. So it has been defaulted to Variant-EFFECTS.",
    "preferred_assay_title": "Variant-EFFECTS",
    "protocols": [
        "https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
    ],
    "release_timestamp": "2023-12-20T23:08:53.303539+00:00",
    "samples": [
        {
            "@id": "/in-vitro-systems/IGVFSM1838USXI/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM1838USXI",
            "aliases": [
                "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep1-FFrep3-BinB"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                {
                    "@id": "/construct-library-sets/IGVFDS2385DHZO/",
                    "accession": "IGVFDS2385DHZO",
                    "file_set_type": "guide library",
                    "status": "in progress",
                    "summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
                }
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0002796/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:Jurkat"
                    ],
                    "status": "released",
                    "summary": "Jurkat",
                    "term_name": "Jurkat"
                }
            ],
            "status": "released",
            "summary": "Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 26-50% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        }
    ],
    "schema_version": "23",
    "status": "released",
    "submitted_by": {
        "@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
        "title": "Michael Montgomery"
    },
    "submitted_files_timestamp": "2023-12-13T09:44:31.202108+00:00",
    "summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
    "targeted_genes": [
        {
            "@id": "/genes/ENSG00000108179/",
            "geneid": "ENSG00000108179",
            "name": "peptidylprolyl isomerase F",
            "status": "released",
            "symbol": "PPIF",
            "synonyms": [
                "rotamase F",
                "CYP3",
                "cyclophilin F",
                "mitochondrial cyclophilin",
                "peptidyl-prolyl cis-trans isomerase, mitochondrial",
                "CypD",
                "Cyp-D",
                "cyclophilin 3",
                "cyclophilin D",
                "CyP-M",
                "peptidyl-prolyl cis-trans isomerase F, mitochondrial",
                "PPIase F"
            ]
        }
    ],
    "uuid": "486481b8-27b4-4eef-96cd-a835536b000a"
}