IGVFDS4487XERQ

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

MYC

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Construct Library Sets

IGVFDS7857SYCK guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR1_Rep1_S28

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR1_Rep2_S83

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR2_Rep1_S34

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR2_Rep2_S89

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR3_Rep1_S40

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR3_Rep2_S95

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR4_Rep1_S46

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR4_Rep2_S101

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9894YYOA

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS6541EVXL

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI1007CYRL, IGVFFI3864GYTT, IGVFFI0409CMRD, IGVFFI8915JKET, IGVFFI8115RHRN, IGVFFI0584UVTC, IGVFFI2004HRIY, IGVFFI7539GUVK

jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR1_Rep1_S28
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR1_Rep2_S83
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR2_Rep1_S34
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR2_Rep2_S89
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR3_Rep1_S40
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR3_Rep2_S95
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR4_Rep1_S46
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR4_Rep2_S101

Jesse Engreitz, Stanford

IGVFDS8214HXSU

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI3328SYVP