Home

IGVFDS4475OZFW

released
File Set Type
experimental data
Summary
CRISPR interference FlowFISH screen sorted on the expression of NOS3 integrating a guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3
Assay Term
in vitro CRISPR screen using flow cytometry
Targeted Genes
Sample Summaries
  • TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin A for 0-25% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
Protocols
Aliases
jesse-engreitz:measurement_NOS3_CRISPRi_FF_R1_FFrep1_binA_PCR1_S113

Sequencing Results (Illumina)

3 items
fastq
reads
I1
1
16 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
15 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
42 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin A for 0-25% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_teloHAEC
female
Homo sapiens

Documents

1 item
Page
Description
Type
Download
Preview
experimental overview
experimental protocol

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford