IGVFDS4315AMOI

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

CCND1

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Construct Library Sets

IGVFDS0392OWNZ guide (sgRNA) library targeting accessible genome regions in 36 genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinF-PCR1_S78

jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinF-PCR2_S174

jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinF-PCR3_S270

jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinF-PCR4_S366

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM4674ZTIR

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS3029MTKI

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci

IGVFFI6162BDDF, IGVFFI0939JLGV, IGVFFI3801VJQA, IGVFFI6403RRAQ

jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinF-PCR1_S78
jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinF-PCR2_S174
jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinF-PCR3_S270
jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinF-PCR4_S366

Jesse Engreitz, Stanford

IGVFDS5292OCBC

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci, ... and 4 more measurement sets

IGVFFI8738YMCU