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IGVFDS4291ANNC
IGVFDS4291ANNC
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This object is in preview status. Metadata is subject to change until this object is released.
File Set Type
experimental data
Summary
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci
Assay Term
in vitro CRISPR screen using single-cell RNA-seq
Sample Summaries
Homo sapiens (female) TeloHAEC cell line modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
Protocols
https://www.protocols.io/view/systematic-perturb-seq-to-discover-endothelial-cel-261ged73yv47/v1
Aliases
jesse-engreitz:measurement_set-scRNAseq_2kG_11AMDox_5
Publications
Convergence of coronary artery disease genes onto endothelial cell programs
Attribution
Jesse Engreitz, Stanford
(
HG011972
—
Jesse Engreitz
)
Sequencing Results (Illumina)
15 items
1
2
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Read Names
Associated seqspec Files
Sequence Specification Document
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI0458PCGP
fastq
reads
R1
3
Illumina NovaSeq 6000
2.8 GB
Jesse Engreitz, Stanford
validated
IGVFFI0819DSEO
fastq
reads
R1
2
Illumina NovaSeq 6000
1.9 GB
Jesse Engreitz, Stanford
validated
IGVFFI1038RHYJ
fastq
reads
R2
5
Illumina NovaSeq 6000
12 GB
Jesse Engreitz, Stanford
validated
IGVFFI1089DXEA
fastq
reads
R2
2
Illumina NovaSeq 6000
2.7 GB
Jesse Engreitz, Stanford
validated
IGVFFI2320OYXN
fastq
reads
R1
1
Illumina NovaSeq 6000
2.2 GB
Jesse Engreitz, Stanford
validated
IGVFFI2683CTJX
fastq
reads
R1
5
Illumina NovaSeq 6000
8.8 GB
Jesse Engreitz, Stanford
validated
IGVFFI3196KOAA
fastq
reads
I1
5
Illumina NovaSeq 6000
2.6 GB
Jesse Engreitz, Stanford
validated
IGVFFI3806RIPV
fastq
reads
I1
1
Illumina NovaSeq 6000
646 MB
Jesse Engreitz, Stanford
validated
IGVFFI4014TMFO
fastq
reads
I1
4
Illumina NovaSeq 6000
359 MB
Jesse Engreitz, Stanford
validated
IGVFFI6406FZZL
fastq
reads
I1
2
Illumina NovaSeq 6000
535 MB
Jesse Engreitz, Stanford
validated
Samples
1 item
Accession
Type
Sample Terms
Associated Construct Library Sets
Summary
IGVFSM2486RPXY
InVitroSystem
TeloHAEC
IGVFDS1298TUYG
Homo sapiens (female) TeloHAEC cell line modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO3459DFTW
igvf:donor_of_teloHAEC
female
Homo sapiens
Associated Construct Library Sets
1 item
Accession
Summary
Library Design Files
IGVFDS1298TUYG
guide (sgRNA) library targeting genes in many genomic loci
IGVFFI9754AGFB
File Sets Using This Measurement Set as an Input
1 item
Accession
Type
Summary
Aliases
Lab
IGVFDS7943IIWZ
AnalysisSet
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci
jesse-engreitz:analysis_set_TeloHAEC_Perturb-seq_2kG
Jesse Engreitz, Stanford
Auxiliary Datasets
1 item
Accession
Type
Summary
Files
Aliases
Lab
IGVFDS3564YOOG
AuxiliarySet
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci
IGVFFI1485EJGT
,
IGVFFI2154ZKGX
,
IGVFFI0629KOLB
,
IGVFFI6969GLRN
jesse-engreitz:auxiliary_sets-scRNAseq_2kG_11AMDox_5
Jesse Engreitz, Stanford