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IGVFDS4291ANNC
IGVFDS4291ANNC
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This object is in preview status. Metadata is subject to change until this object is released.
File Set Type
experimental data
Summary
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci
Assay Term
in vitro CRISPR screen using single-cell RNA-seq
Sample Summaries
Homo sapiens (female) TeloHAEC cell line modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
Protocols
https://www.protocols.io/view/systematic-perturb-seq-to-discover-endothelial-cel-261ged73yv47/v1
Aliases
jesse-engreitz:measurement_set-scRNAseq_2kG_11AMDox_5
Publications
Convergence of coronary artery disease genes onto endothelial cell programs
Attribution
Jesse Engreitz, Stanford
(
HG011972
—
Jesse Engreitz
)
Sequencing Results (Illumina)
15 items
1
2
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Read Names
Associated seqspec Files
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI0458PCGP
fastq
reads
R1
3
Illumina NovaSeq 6000
2.8 GB
Jesse Engreitz, Stanford
validated
IGVFFI0819DSEO
fastq
reads
R1
2
Illumina NovaSeq 6000
1.9 GB
Jesse Engreitz, Stanford
validated
IGVFFI1038RHYJ
fastq
reads
R2
5
Illumina NovaSeq 6000
12 GB
Jesse Engreitz, Stanford
validated
IGVFFI1089DXEA
fastq
reads
R2
2
Illumina NovaSeq 6000
2.7 GB
Jesse Engreitz, Stanford
validated
IGVFFI2320OYXN
fastq
reads
R1
1
Illumina NovaSeq 6000
2.2 GB
Jesse Engreitz, Stanford
validated
IGVFFI2683CTJX
fastq
reads
R1
5
Illumina NovaSeq 6000
8.8 GB
Jesse Engreitz, Stanford
validated
IGVFFI3196KOAA
fastq
reads
I1
5
Illumina NovaSeq 6000
2.6 GB
Jesse Engreitz, Stanford
validated
IGVFFI3806RIPV
fastq
reads
I1
1
Illumina NovaSeq 6000
646 MB
Jesse Engreitz, Stanford
validated
IGVFFI4014TMFO
fastq
reads
I1
4
Illumina NovaSeq 6000
359 MB
Jesse Engreitz, Stanford
validated
IGVFFI6406FZZL
fastq
reads
I1
2
Illumina NovaSeq 6000
535 MB
Jesse Engreitz, Stanford
validated
Samples
1 item
Accession
Type
Sample Terms
Associated Construct Library Sets
Summary
IGVFSM2486RPXY
InVitroSystem
TeloHAEC
IGVFDS1298TUYG
Homo sapiens (female) TeloHAEC cell line modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO3459DFTW
igvf:donor_of_teloHAEC
female
Homo sapiens
Associated Construct Library Sets
1 item
Accession
Summary
Library Design Files
IGVFDS1298TUYG
guide (sgRNA) library targeting genes in many genomic loci
IGVFFI9754AGFB
File Sets Using This Measurement Set as an Input
1 item
Accession
Type
Summary
Aliases
Lab
IGVFDS7943IIWZ
AnalysisSet
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci
jesse-engreitz:analysis_set_TeloHAEC_Perturb-seq_2kG
Jesse Engreitz, Stanford
Auxiliary Datasets
1 item
Accession
Type
Summary
Files
Aliases
Lab
IGVFDS3564YOOG
AuxiliarySet
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci
IGVFFI1485EJGT
,
IGVFFI2154ZKGX
,
IGVFFI0629KOLB
,
IGVFFI6969GLRN
jesse-engreitz:auxiliary_sets-scRNAseq_2kG_11AMDox_5
Jesse Engreitz, Stanford