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IGVFDS4213FGQT

released
File Set Type
experimental data
Summary
CRISPR interference FACS screen sorted on the expression of B2M integrating a guide (sgRNA) library targeting transcription start sites in B2M
Assay Term
in vitro CRISPR screen using flow cytometry
Targeted Genes
Sample Summaries
  • Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of b2m) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)
Protocols
Aliases
charles-gersbach:cd8-tcells-measurement-set-crispri-b2m-high-bin-rep1
Publications
Attribution
Charles Gersbach, Duke

Sequencing Results (Illumina)

1 item
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Read Names
Associated seqspec Files
Sequence Specification Document
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
fastq
reads
R1
1
000000000-J9HPF
1
20 MB
Charles Gersbach, Duke
validated

Samples

1 item
Accession
Type
Sample Terms
Associated Construct Library Sets
Summary
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of b2m) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)

Donors

1 item
Accession
Aliases
Sex
Taxa
  • charles-gersbach:cd8-tcells-donor-17
unspecified
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
guide (sgRNA) library targeting transcription start sites in B2M

Control File Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
low FACS signal CRISPR interference FACS screen sorted on the expression of B2M integrating a guide (sgRNA) library targeting transcription start sites in B2M
  • charles-gersbach:cd8-tcells-measurement-set-crispri-b2m-low-bin-rep1
Charles Gersbach, Duke

File Sets Using This Measurement Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
CRISPR interference FACS screen targeting B2M integrating a guide (sgRNA) library targeting transcription start sites in B2M with low FACS signal control
  • charles-gersbach:cd8-tcells-analysis-set-crispri-b2m
Charles Gersbach, Duke