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IGVFDS4213FGQT

released
File Set Type
experimental data
Summary
CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in B2M
Assay Term
in vitro CRISPR screen using flow cytometry
Targeted Genes
Sample Summaries
  • CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
Construct Library Sets
  • IGVFDS5026WRUO guide (sgRNA) library targeting transcription start sites in B2M
Protocols
Aliases
charles-gersbach:cd8-tcells-measurement-set-crispri-b2m-high-bin-rep1
Publications

Samples

1 item
Accession
Type
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Donors

1 item
Accession
Aliases
Sex
Taxa
  • charles-gersbach:cd8-tcells-donor-17
unspecified
Homo sapiens

Control File Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in B2M
  • charles-gersbach:cd8-tcells-measurement-set-crispri-b2m-low-bin-rep1
Charles Gersbach, Duke

Auxiliary Datasets

1 item
Accession
Type
Summary
Files
Aliases
Lab
AuxiliarySet
gRNA sequencing for CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in B2M
  • charles-gersbach:cd8-tcells-auxiliary-set-crispri-b2m-high-bin-rep1
Charles Gersbach, Duke

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke