{
"@context": "/terms/",
"@id": "/measurement-sets/IGVFDS4141QOFR/",
"@type": [
"MeasurementSet",
"FileSet",
"Item"
],
"accession": "IGVFDS4141QOFR",
"aliases": [
"jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep2-FFrep3-BinA_AmpliconSequencing"
],
"assay_term": {
"@id": "/assay-terms/OBI_0003661/",
"status": "released",
"term_name": "in vitro CRISPR screen using flow cytometry"
},
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Released in vitro system [IGVFSM0259SMPF](/in-vitro-systems/IGVFSM0259SMPF/) has in progress subobject construct library set [IGVFDS2385DHZO](/construct-library-sets/IGVFDS2385DHZO/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/in-vitro-systems/IGVFSM0259SMPF/"
},
{
"category": "missing sequencing kit",
"detail": "Measurement set [IGVFDS4141QOFR](/measurement-sets/IGVFDS4141QOFR/) has sequence file(s) [IGVFFI8021ERKN](/sequence-files/IGVFFI8021ERKN/), [IGVFFI1588VVFK](/sequence-files/IGVFFI1588VVFK/), [IGVFFI2245ZYZS](/sequence-files/IGVFFI2245ZYZS/), [IGVFFI4834ZJAB](/sequence-files/IGVFFI4834ZJAB/), [IGVFFI6001DMDJ](/sequence-files/IGVFFI6001DMDJ/), [IGVFFI3374SFEY](/sequence-files/IGVFFI3374SFEY/), [IGVFFI0256WAGY](/sequence-files/IGVFFI0256WAGY/), [IGVFFI2777HKED](/sequence-files/IGVFFI2777HKED/), [IGVFFI3790CZIF](/sequence-files/IGVFFI3790CZIF/), [IGVFFI4099OVNZ](/sequence-files/IGVFFI4099OVNZ/), [IGVFFI1738HIPM](/sequence-files/IGVFFI1738HIPM/), [IGVFFI1429FYEG](/sequence-files/IGVFFI1429FYEG/), [IGVFFI7917KPBJ](/sequence-files/IGVFFI7917KPBJ/), [IGVFFI3271BSJD](/sequence-files/IGVFFI3271BSJD/), [IGVFFI4480KATX](/sequence-files/IGVFFI4480KATX/), [IGVFFI1252TTRQ](/sequence-files/IGVFFI1252TTRQ/), [IGVFFI5887KXBV](/sequence-files/IGVFFI5887KXBV/), [IGVFFI6910SIEW](/sequence-files/IGVFFI6910SIEW/), [IGVFFI9778KPAU](/sequence-files/IGVFFI9778KPAU/), [IGVFFI3157QDGB](/sequence-files/IGVFFI3157QDGB/), [IGVFFI1047ZJWY](/sequence-files/IGVFFI1047ZJWY/), [IGVFFI4965BEGQ](/sequence-files/IGVFFI4965BEGQ/), [IGVFFI1295YHHN](/sequence-files/IGVFFI1295YHHN/), [IGVFFI6716WRFX](/sequence-files/IGVFFI6716WRFX/), [IGVFFI2032BIJT](/sequence-files/IGVFFI2032BIJT/), [IGVFFI8471XDOD](/sequence-files/IGVFFI8471XDOD/), [IGVFFI6826TTLX](/sequence-files/IGVFFI6826TTLX/), [IGVFFI0557KAVN](/sequence-files/IGVFFI0557KAVN/), [IGVFFI5398ROAM](/sequence-files/IGVFFI5398ROAM/), [IGVFFI2015ODUP](/sequence-files/IGVFFI2015ODUP/), [IGVFFI8185JOGB](/sequence-files/IGVFFI8185JOGB/), [IGVFFI0789NOIT](/sequence-files/IGVFFI0789NOIT/), [IGVFFI2874QKKT](/sequence-files/IGVFFI2874QKKT/), [IGVFFI0906QXQF](/sequence-files/IGVFFI0906QXQF/), [IGVFFI4244WXGM](/sequence-files/IGVFFI4244WXGM/), [IGVFFI2586IXAF](/sequence-files/IGVFFI2586IXAF/), [IGVFFI2731JTVH](/sequence-files/IGVFFI2731JTVH/), [IGVFFI3018SSEY](/sequence-files/IGVFFI3018SSEY/), [IGVFFI2444LDZO](/sequence-files/IGVFFI2444LDZO/), [IGVFFI3799BWTY](/sequence-files/IGVFFI3799BWTY/), [IGVFFI4472MLSZ](/sequence-files/IGVFFI4472MLSZ/), [IGVFFI8798QLWP](/sequence-files/IGVFFI8798QLWP/), [IGVFFI3831ITLD](/sequence-files/IGVFFI3831ITLD/), [IGVFFI5310QJQU](/sequence-files/IGVFFI5310QJQU/), [IGVFFI0220HAFC](/sequence-files/IGVFFI0220HAFC/), [IGVFFI7244WSOU](/sequence-files/IGVFFI7244WSOU/), [IGVFFI1969NNZC](/sequence-files/IGVFFI1969NNZC/), [IGVFFI2390PFXK](/sequence-files/IGVFFI2390PFXK/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_inconsistent_sequencing_kit",
"path": "/measurement-sets/IGVFDS4141QOFR/"
},
{
"category": "missing sequence specification",
"detail": "Measurement set [IGVFDS4141QOFR](/measurement-sets/IGVFDS4141QOFR/) has sequence file(s): [IGVFFI8021ERKN](/sequence-files/IGVFFI8021ERKN/), [IGVFFI1588VVFK](/sequence-files/IGVFFI1588VVFK/), [IGVFFI2245ZYZS](/sequence-files/IGVFFI2245ZYZS/), [IGVFFI4834ZJAB](/sequence-files/IGVFFI4834ZJAB/), [IGVFFI6001DMDJ](/sequence-files/IGVFFI6001DMDJ/), [IGVFFI3374SFEY](/sequence-files/IGVFFI3374SFEY/), [IGVFFI0256WAGY](/sequence-files/IGVFFI0256WAGY/), [IGVFFI2777HKED](/sequence-files/IGVFFI2777HKED/), [IGVFFI3790CZIF](/sequence-files/IGVFFI3790CZIF/), [IGVFFI4099OVNZ](/sequence-files/IGVFFI4099OVNZ/), [IGVFFI1738HIPM](/sequence-files/IGVFFI1738HIPM/), [IGVFFI1429FYEG](/sequence-files/IGVFFI1429FYEG/), [IGVFFI7917KPBJ](/sequence-files/IGVFFI7917KPBJ/), [IGVFFI3271BSJD](/sequence-files/IGVFFI3271BSJD/), [IGVFFI4480KATX](/sequence-files/IGVFFI4480KATX/), [IGVFFI1252TTRQ](/sequence-files/IGVFFI1252TTRQ/), [IGVFFI5887KXBV](/sequence-files/IGVFFI5887KXBV/), [IGVFFI6910SIEW](/sequence-files/IGVFFI6910SIEW/), [IGVFFI9778KPAU](/sequence-files/IGVFFI9778KPAU/), [IGVFFI3157QDGB](/sequence-files/IGVFFI3157QDGB/), [IGVFFI1047ZJWY](/sequence-files/IGVFFI1047ZJWY/), [IGVFFI4965BEGQ](/sequence-files/IGVFFI4965BEGQ/), [IGVFFI1295YHHN](/sequence-files/IGVFFI1295YHHN/), [IGVFFI6716WRFX](/sequence-files/IGVFFI6716WRFX/), [IGVFFI2032BIJT](/sequence-files/IGVFFI2032BIJT/), [IGVFFI8471XDOD](/sequence-files/IGVFFI8471XDOD/), [IGVFFI6826TTLX](/sequence-files/IGVFFI6826TTLX/), [IGVFFI0557KAVN](/sequence-files/IGVFFI0557KAVN/), [IGVFFI5398ROAM](/sequence-files/IGVFFI5398ROAM/), [IGVFFI2015ODUP](/sequence-files/IGVFFI2015ODUP/), [IGVFFI8185JOGB](/sequence-files/IGVFFI8185JOGB/), [IGVFFI0789NOIT](/sequence-files/IGVFFI0789NOIT/), [IGVFFI2874QKKT](/sequence-files/IGVFFI2874QKKT/), [IGVFFI0906QXQF](/sequence-files/IGVFFI0906QXQF/), [IGVFFI4244WXGM](/sequence-files/IGVFFI4244WXGM/), [IGVFFI2586IXAF](/sequence-files/IGVFFI2586IXAF/), [IGVFFI2731JTVH](/sequence-files/IGVFFI2731JTVH/), [IGVFFI3018SSEY](/sequence-files/IGVFFI3018SSEY/), [IGVFFI2444LDZO](/sequence-files/IGVFFI2444LDZO/), [IGVFFI3799BWTY](/sequence-files/IGVFFI3799BWTY/), [IGVFFI4472MLSZ](/sequence-files/IGVFFI4472MLSZ/), [IGVFFI8798QLWP](/sequence-files/IGVFFI8798QLWP/), [IGVFFI3831ITLD](/sequence-files/IGVFFI3831ITLD/), [IGVFFI5310QJQU](/sequence-files/IGVFFI5310QJQU/), [IGVFFI0220HAFC](/sequence-files/IGVFFI0220HAFC/), [IGVFFI7244WSOU](/sequence-files/IGVFFI7244WSOU/), [IGVFFI1969NNZC](/sequence-files/IGVFFI1969NNZC/), [IGVFFI2390PFXK](/sequence-files/IGVFFI2390PFXK/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_missing_seqspec",
"path": "/measurement-sets/IGVFDS4141QOFR/"
}
],
"WARNING": [
{
"category": "missing auxiliary set",
"detail": "Measurement set [IGVFDS4141QOFR](/measurement-sets/IGVFDS4141QOFR/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
"level": 40,
"level_name": "WARNING",
"name": "audit_missing_auxiliary_set",
"path": "/measurement-sets/IGVFDS4141QOFR/"
}
]
},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
"title": "Jesse Engreitz"
},
"title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
},
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{
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],
"status": "released"
}
],
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{
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"accession": "IGVFDO7359LVPI",
"aliases": [
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],
"sex": "male",
"status": "released",
"taxa": "Homo sapiens"
}
],
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"@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
"title": "Michael Montgomery"
},
"submitted_files_timestamp": "2023-12-13T09:07:05.091798+00:00",
"summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
"targeted_genes": [
{
"@id": "/genes/ENSG00000108179/",
"geneid": "ENSG00000108179",
"name": "peptidylprolyl isomerase F",
"status": "released",
"symbol": "PPIF",
"synonyms": [
"rotamase F",
"CYP3",
"cyclophilin F",
"mitochondrial cyclophilin",
"peptidyl-prolyl cis-trans isomerase, mitochondrial",
"CypD",
"Cyp-D",
"cyclophilin 3",
"cyclophilin D",
"CyP-M",
"peptidyl-prolyl cis-trans isomerase F, mitochondrial",
"PPIase F"
]
}
],
"uuid": "b19678ee-d555-4a83-a8fd-f45cc4ab7c16"
}