IGVFDS3872CUHM

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

ITPRID2

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Construct Library Sets

IGVFDS5413RKIA guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinF-PCR1-Rep1_S18

jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinF-PCR2-Rep1_S24

jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinF-PCR3-Rep1_S72

jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinF-PCR4-Rep1_S78

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM4941RWXR

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS2934UZUA

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI5535XSUG, IGVFFI5233CFBC, IGVFFI0372HBLG, IGVFFI3691CGEM

jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin0hrs-FF2-BinF-PCR1-Rep1_S18
jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin0hrs-FF2-BinF-PCR2-Rep1_S24
jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin0hrs-FF2-BinF-PCR3-Rep1_S72
jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin0hrs-FF2-BinF-PCR4-Rep1_S78

Jesse Engreitz, Stanford

IGVFDS4597CMXX

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI5802FOEM