IGVFDS3765SWWC

released
{
    "@context": "/terms/",
    "@id": "/measurement-sets/IGVFDS3765SWWC/",
    "@type": [
        "MeasurementSet",
        "FileSet",
        "Item"
    ],
    "accession": "IGVFDS3765SWWC",
    "aliases": [
        "jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep2-FFrep4-BinA_AmpliconSequencing"
    ],
    "assay_term": {
        "@id": "/assay-terms/OBI_0003661/",
        "status": "released",
        "term_name": "in vitro CRISPR screen using flow cytometry"
    },
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "mismatched status",
                "detail": "Released in vitro system [IGVFSM2557IAGR](/in-vitro-systems/IGVFSM2557IAGR/) has in progress subobject construct library set [IGVFDS2385DHZO](/construct-library-sets/IGVFDS2385DHZO/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/in-vitro-systems/IGVFSM2557IAGR/"
            },
            {
                "category": "missing sequencing kit",
                "detail": "Measurement set [IGVFDS3765SWWC](/measurement-sets/IGVFDS3765SWWC/) has sequence file(s) [IGVFFI2510ASDE](/sequence-files/IGVFFI2510ASDE/), [IGVFFI0601XPFY](/sequence-files/IGVFFI0601XPFY/), [IGVFFI1424IKFY](/sequence-files/IGVFFI1424IKFY/), [IGVFFI0760ZZUA](/sequence-files/IGVFFI0760ZZUA/), [IGVFFI6249UMFI](/sequence-files/IGVFFI6249UMFI/), [IGVFFI5710TBVP](/sequence-files/IGVFFI5710TBVP/), [IGVFFI8170ELEN](/sequence-files/IGVFFI8170ELEN/), [IGVFFI5845SYKV](/sequence-files/IGVFFI5845SYKV/), [IGVFFI4139XIUZ](/sequence-files/IGVFFI4139XIUZ/), [IGVFFI1585IGGH](/sequence-files/IGVFFI1585IGGH/), [IGVFFI6987FQET](/sequence-files/IGVFFI6987FQET/), [IGVFFI8980OZIR](/sequence-files/IGVFFI8980OZIR/), [IGVFFI7476FAEF](/sequence-files/IGVFFI7476FAEF/), [IGVFFI9366IRCH](/sequence-files/IGVFFI9366IRCH/), [IGVFFI3902DBNN](/sequence-files/IGVFFI3902DBNN/), [IGVFFI3155EKEY](/sequence-files/IGVFFI3155EKEY/), [IGVFFI6268SEMT](/sequence-files/IGVFFI6268SEMT/), [IGVFFI5420GUHQ](/sequence-files/IGVFFI5420GUHQ/), [IGVFFI4925QRTR](/sequence-files/IGVFFI4925QRTR/), [IGVFFI1800NRAG](/sequence-files/IGVFFI1800NRAG/), [IGVFFI1667VREQ](/sequence-files/IGVFFI1667VREQ/), [IGVFFI4271CIHQ](/sequence-files/IGVFFI4271CIHQ/), [IGVFFI5496HCDH](/sequence-files/IGVFFI5496HCDH/), [IGVFFI3826WHUR](/sequence-files/IGVFFI3826WHUR/), [IGVFFI7392SJRB](/sequence-files/IGVFFI7392SJRB/), [IGVFFI5177VTTC](/sequence-files/IGVFFI5177VTTC/), [IGVFFI7239FHHM](/sequence-files/IGVFFI7239FHHM/), [IGVFFI0617MYPV](/sequence-files/IGVFFI0617MYPV/), [IGVFFI4537FCCI](/sequence-files/IGVFFI4537FCCI/), [IGVFFI7027GQXC](/sequence-files/IGVFFI7027GQXC/), [IGVFFI8994SLCX](/sequence-files/IGVFFI8994SLCX/), [IGVFFI8651DLZB](/sequence-files/IGVFFI8651DLZB/), [IGVFFI1426JKWP](/sequence-files/IGVFFI1426JKWP/), [IGVFFI3161DWEU](/sequence-files/IGVFFI3161DWEU/), [IGVFFI9051TCCZ](/sequence-files/IGVFFI9051TCCZ/), [IGVFFI4650NLWV](/sequence-files/IGVFFI4650NLWV/), [IGVFFI8671NHNC](/sequence-files/IGVFFI8671NHNC/), [IGVFFI4563ZWDG](/sequence-files/IGVFFI4563ZWDG/), [IGVFFI2813ARMS](/sequence-files/IGVFFI2813ARMS/), [IGVFFI3587LMNL](/sequence-files/IGVFFI3587LMNL/), [IGVFFI6151SNGD](/sequence-files/IGVFFI6151SNGD/), [IGVFFI8435LCYS](/sequence-files/IGVFFI8435LCYS/), [IGVFFI7807MTEI](/sequence-files/IGVFFI7807MTEI/), [IGVFFI6728VMSV](/sequence-files/IGVFFI6728VMSV/), [IGVFFI6865FKIY](/sequence-files/IGVFFI6865FKIY/), [IGVFFI8363TESH](/sequence-files/IGVFFI8363TESH/), [IGVFFI2673CTYV](/sequence-files/IGVFFI2673CTYV/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/measurement-sets/IGVFDS3765SWWC/"
            },
            {
                "category": "missing sequence specification",
                "detail": "Measurement set [IGVFDS3765SWWC](/measurement-sets/IGVFDS3765SWWC/) has sequence file(s): [IGVFFI2510ASDE](/sequence-files/IGVFFI2510ASDE/), [IGVFFI0601XPFY](/sequence-files/IGVFFI0601XPFY/), [IGVFFI1424IKFY](/sequence-files/IGVFFI1424IKFY/), [IGVFFI0760ZZUA](/sequence-files/IGVFFI0760ZZUA/), [IGVFFI6249UMFI](/sequence-files/IGVFFI6249UMFI/), [IGVFFI5710TBVP](/sequence-files/IGVFFI5710TBVP/), [IGVFFI8170ELEN](/sequence-files/IGVFFI8170ELEN/), [IGVFFI5845SYKV](/sequence-files/IGVFFI5845SYKV/), [IGVFFI4139XIUZ](/sequence-files/IGVFFI4139XIUZ/), [IGVFFI1585IGGH](/sequence-files/IGVFFI1585IGGH/), [IGVFFI6987FQET](/sequence-files/IGVFFI6987FQET/), [IGVFFI8980OZIR](/sequence-files/IGVFFI8980OZIR/), [IGVFFI7476FAEF](/sequence-files/IGVFFI7476FAEF/), [IGVFFI9366IRCH](/sequence-files/IGVFFI9366IRCH/), [IGVFFI3902DBNN](/sequence-files/IGVFFI3902DBNN/), [IGVFFI3155EKEY](/sequence-files/IGVFFI3155EKEY/), [IGVFFI6268SEMT](/sequence-files/IGVFFI6268SEMT/), [IGVFFI5420GUHQ](/sequence-files/IGVFFI5420GUHQ/), [IGVFFI4925QRTR](/sequence-files/IGVFFI4925QRTR/), [IGVFFI1800NRAG](/sequence-files/IGVFFI1800NRAG/), [IGVFFI1667VREQ](/sequence-files/IGVFFI1667VREQ/), [IGVFFI4271CIHQ](/sequence-files/IGVFFI4271CIHQ/), [IGVFFI5496HCDH](/sequence-files/IGVFFI5496HCDH/), [IGVFFI3826WHUR](/sequence-files/IGVFFI3826WHUR/), [IGVFFI7392SJRB](/sequence-files/IGVFFI7392SJRB/), [IGVFFI5177VTTC](/sequence-files/IGVFFI5177VTTC/), [IGVFFI7239FHHM](/sequence-files/IGVFFI7239FHHM/), [IGVFFI0617MYPV](/sequence-files/IGVFFI0617MYPV/), [IGVFFI4537FCCI](/sequence-files/IGVFFI4537FCCI/), [IGVFFI7027GQXC](/sequence-files/IGVFFI7027GQXC/), [IGVFFI8994SLCX](/sequence-files/IGVFFI8994SLCX/), [IGVFFI8651DLZB](/sequence-files/IGVFFI8651DLZB/), [IGVFFI1426JKWP](/sequence-files/IGVFFI1426JKWP/), [IGVFFI3161DWEU](/sequence-files/IGVFFI3161DWEU/), [IGVFFI9051TCCZ](/sequence-files/IGVFFI9051TCCZ/), [IGVFFI4650NLWV](/sequence-files/IGVFFI4650NLWV/), [IGVFFI8671NHNC](/sequence-files/IGVFFI8671NHNC/), [IGVFFI4563ZWDG](/sequence-files/IGVFFI4563ZWDG/), [IGVFFI2813ARMS](/sequence-files/IGVFFI2813ARMS/), [IGVFFI3587LMNL](/sequence-files/IGVFFI3587LMNL/), [IGVFFI6151SNGD](/sequence-files/IGVFFI6151SNGD/), [IGVFFI8435LCYS](/sequence-files/IGVFFI8435LCYS/), [IGVFFI7807MTEI](/sequence-files/IGVFFI7807MTEI/), [IGVFFI6728VMSV](/sequence-files/IGVFFI6728VMSV/), [IGVFFI6865FKIY](/sequence-files/IGVFFI6865FKIY/), [IGVFFI8363TESH](/sequence-files/IGVFFI8363TESH/), [IGVFFI2673CTYV](/sequence-files/IGVFFI2673CTYV/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_missing_seqspec",
                "path": "/measurement-sets/IGVFDS3765SWWC/"
            }
        ],
        "WARNING": [
            {
                "category": "missing auxiliary set",
                "detail": "Measurement set [IGVFDS3765SWWC](/measurement-sets/IGVFDS3765SWWC/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_missing_auxiliary_set",
                "path": "/measurement-sets/IGVFDS3765SWWC/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization",
        "contact_pi": {
            "@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
            "title": "Jesse Engreitz"
        },
        "title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
    },
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            "@id": "/measurement-sets/IGVFDS5128CXIS/",
            "accession": "IGVFDS5128CXIS",
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                "jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-THP1-PE2_Exp4_NegControl_AmpliconSequencing"
            ],
            "status": "released"
        }
    ],
    "control_for": [],
    "creation_timestamp": "2023-12-13T08:01:47.842444+00:00",
    "donors": [
        {
            "@id": "/human-donors/IGVFDO7359LVPI/",
            "accession": "IGVFDO7359LVPI",
            "aliases": [
                "igvf:donor_of_THP1"
            ],
            "sex": "male",
            "status": "released",
            "taxa": "Homo sapiens"
        }
    ],
    "externally_hosted": false,
    "file_set_type": "experimental data",
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    "preferred_assay_title": "Variant-EFFECTS",
    "protocols": [
        "https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
    ],
    "release_timestamp": "2023-12-20T23:07:19.788125+00:00",
    "samples": [
        {
            "@id": "/in-vitro-systems/IGVFSM2557IAGR/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM2557IAGR",
            "aliases": [
                "jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep2-FFrep4-BinA"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                {
                    "@id": "/construct-library-sets/IGVFDS2385DHZO/",
                    "accession": "IGVFDS2385DHZO",
                    "file_set_type": "guide library",
                    "status": "in progress",
                    "summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
                }
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0001253/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:THP-1"
                    ],
                    "status": "released",
                    "summary": "THP-1",
                    "term_name": "THP-1"
                }
            ],
            "status": "released",
            "summary": "THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        }
    ],
    "schema_version": "23",
    "status": "released",
    "submitted_by": {
        "@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
        "title": "Michael Montgomery"
    },
    "submitted_files_timestamp": "2023-12-13T09:15:30.255398+00:00",
    "summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
    "targeted_genes": [
        {
            "@id": "/genes/ENSG00000108179/",
            "geneid": "ENSG00000108179",
            "name": "peptidylprolyl isomerase F",
            "status": "released",
            "symbol": "PPIF",
            "synonyms": [
                "rotamase F",
                "CYP3",
                "cyclophilin F",
                "mitochondrial cyclophilin",
                "peptidyl-prolyl cis-trans isomerase, mitochondrial",
                "CypD",
                "Cyp-D",
                "cyclophilin 3",
                "cyclophilin D",
                "CyP-M",
                "peptidyl-prolyl cis-trans isomerase F, mitochondrial",
                "PPIase F"
            ]
        }
    ],
    "uuid": "e950d39b-28cd-4837-bea2-add0e6ce5e5c"
}