{
"@context": "/terms/",
"@id": "/measurement-sets/IGVFDS3659HSOV/",
"@type": [
"MeasurementSet",
"FileSet",
"Item"
],
"accession": "IGVFDS3659HSOV",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep3-FFrep3-BinC_AmpliconSequencing"
],
"assay_term": {
"@id": "/assay-terms/OBI_0003661/",
"assay_slims": [
"CRISPR screens"
],
"status": "released",
"term_name": "in vitro CRISPR screen using flow cytometry"
},
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Released in vitro system [IGVFSM8988WXEB](/in-vitro-systems/IGVFSM8988WXEB/) has in progress subobject construct library set [IGVFDS6377KNCH](/construct-library-sets/IGVFDS6377KNCH/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/in-vitro-systems/IGVFSM8988WXEB/"
},
{
"category": "missing publication",
"detail": "In vitro system [IGVFSM8988WXEB](/in-vitro-systems/IGVFSM8988WXEB/) has no `publications`. Released and archived samples are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_sample_missing_publication",
"path": "/in-vitro-systems/IGVFSM8988WXEB/"
},
{
"category": "missing publication",
"detail": "Measurement set [IGVFDS3659HSOV](/measurement-sets/IGVFDS3659HSOV/) has no `publications`. Released and archived file sets are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_file_set_missing_publication",
"path": "/measurement-sets/IGVFDS3659HSOV/"
},
{
"category": "missing sequencing kit",
"detail": "Measurement set [IGVFDS3659HSOV](/measurement-sets/IGVFDS3659HSOV/) has sequence file(s) [IGVFFI9303OZUT](/sequence-files/IGVFFI9303OZUT/), [IGVFFI5941OVJF](/sequence-files/IGVFFI5941OVJF/), [IGVFFI0897ITLM](/sequence-files/IGVFFI0897ITLM/), [IGVFFI7681MCEO](/sequence-files/IGVFFI7681MCEO/), [IGVFFI3878VUNO](/sequence-files/IGVFFI3878VUNO/), [IGVFFI9633MOEW](/sequence-files/IGVFFI9633MOEW/), [IGVFFI3999AYTL](/sequence-files/IGVFFI3999AYTL/), [IGVFFI5965WIAG](/sequence-files/IGVFFI5965WIAG/) which lack specification of a `sequencing_kit`. Sequence files in a file set associated wtih bulk data should specify a sequencing kit.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_inconsistent_sequencing_kit",
"path": "/measurement-sets/IGVFDS3659HSOV/"
},
{
"category": "missing sequence specification",
"detail": "Measurement set [IGVFDS3659HSOV](/measurement-sets/IGVFDS3659HSOV/) has sequence file(s): [IGVFFI9303OZUT](/sequence-files/IGVFFI9303OZUT/), [IGVFFI5941OVJF](/sequence-files/IGVFFI5941OVJF/), [IGVFFI0897ITLM](/sequence-files/IGVFFI0897ITLM/), [IGVFFI7681MCEO](/sequence-files/IGVFFI7681MCEO/), [IGVFFI3878VUNO](/sequence-files/IGVFFI3878VUNO/), [IGVFFI9633MOEW](/sequence-files/IGVFFI9633MOEW/), [IGVFFI3999AYTL](/sequence-files/IGVFFI3999AYTL/), [IGVFFI5965WIAG](/sequence-files/IGVFFI5965WIAG/) which do not have any `seqspecs`. Sequence files in a file set associated with bulk data are expected to link to a sequence specification file.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_missing_seqspec",
"path": "/measurement-sets/IGVFDS3659HSOV/"
}
],
"WARNING": [
{
"category": "missing auxiliary set",
"detail": "Measurement set [IGVFDS3659HSOV](/measurement-sets/IGVFDS3659HSOV/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
"level": 40,
"level_name": "WARNING",
"name": "audit_missing_auxiliary_set",
"path": "/measurement-sets/IGVFDS3659HSOV/"
}
]
},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
"title": "Jesse Engreitz"
},
"title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
},
"construct_library_sets": [
{
"@id": "/construct-library-sets/IGVFDS6377KNCH/",
"accession": "IGVFDS6377KNCH",
"file_set_type": "guide library",
"status": "in progress",
"summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
}
],
"control_file_sets": [
{
"@id": "/measurement-sets/IGVFDS6385JXFZ/",
"accession": "IGVFDS6385JXFZ",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-THP1-PE2_Exp3_NegControl_AmpliconSequencing"
],
"status": "released"
}
],
"control_for": [],
"creation_timestamp": "2023-11-30T22:46:38.282959+00:00",
"data_use_limitation_summaries": [
"no certificate"
],
"donors": [
{
"@id": "/human-donors/IGVFDO7359LVPI/",
"accession": "IGVFDO7359LVPI",
"aliases": [
"igvf:donor_of_THP1"
],
"sex": "male",
"status": "released",
"taxa": "Homo sapiens"
}
],
"externally_hosted": false,
"file_set_type": "experimental data",
"files": [
{
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"accession": "IGVFFI9303OZUT",
"aliases": [
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],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T19:09:05.916610+00:00",
"file_format": "fastq",
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"sequencing_platform": {
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"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220426pPPIF_REPEAT-THP1-Gen8b-pPPIF-Amp_pPPIF-BioRep1-FFRep3-BinC-PCRrep1_S17_I2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI5941OVJF/",
"accession": "IGVFFI5941OVJF",
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],
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"controlled_access": false,
"creation_timestamp": "2023-12-05T18:31:08.707529+00:00",
"file_format": "fastq",
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"href": "/sequence-files/IGVFFI5941OVJF/@@download/IGVFFI5941OVJF.fastq.gz",
"md5sum": "48637fae62dc7f33d2d3bf56bd9aadd3",
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"sequencing_platform": {
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"status": "released",
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"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI0897ITLM/",
"accession": "IGVFFI0897ITLM",
"aliases": [
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],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T18:50:14.184812+00:00",
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"href": "/sequence-files/IGVFFI0897ITLM/@@download/IGVFFI0897ITLM.fastq.gz",
"md5sum": "d1088a6427f84dd65656568442813b26",
"s3_uri": "s3://igvf-files/2023/12/05/f1e13d15-b9d4-4bde-825e-2f0ca93296c8/IGVFFI0897ITLM.fastq.gz",
"sequencing_platform": {
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"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220426pPPIF_REPEAT-THP1-Gen8b-pPPIF-Amp_pPPIF-BioRep1-FFRep3-BinC-PCRrep2_S113_R2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI7681MCEO/",
"accession": "IGVFFI7681MCEO",
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"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep3-FFrep3-BinC-PCRrep2_R1"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T18:37:28.164310+00:00",
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"md5sum": "c094d66b3b1131af2ab8de9cf2adc082",
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"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI3878VUNO/",
"accession": "IGVFFI3878VUNO",
"aliases": [
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],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T18:56:39.875810+00:00",
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"md5sum": "a0c79792b0118150d98327f62cc738ed",
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"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220426pPPIF_REPEAT-THP1-Gen8b-pPPIF-Amp_pPPIF-BioRep1-FFRep3-BinC-PCRrep1_S17_I1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI9633MOEW/",
"accession": "IGVFFI9633MOEW",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep3-FFrep3-BinC-PCRrep1_R2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T18:43:49.689919+00:00",
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},
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},
{
"@id": "/sequence-files/IGVFFI3999AYTL/",
"accession": "IGVFFI3999AYTL",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep3-FFrep3-BinC-PCRrep2_I2"
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},
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},
{
"@id": "/sequence-files/IGVFFI5965WIAG/",
"accession": "IGVFFI5965WIAG",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep3-FFrep3-BinC-PCRrep2_I1"
],
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"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220426pPPIF_REPEAT-THP1-Gen8b-pPPIF-Amp_pPPIF-BioRep1-FFRep3-BinC-PCRrep2_S113_I1_001.fastq.gz",
"upload_status": "validated"
}
],
"input_for": [
"/analysis-sets/IGVFDS5056OAGR/"
],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set. This measurement set previously used Variant FlowFISH as a preferred_assay_title, but this enum is now removed. So it has been defaulted to Variant-EFFECTS.",
"preferred_assay_title": "Variant-EFFECTS",
"protocols": [
"https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
],
"release_timestamp": "2023-12-20T23:09:44.445091+00:00",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM8988WXEB/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM8988WXEB",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep3-FFrep3-BinC"
],
"classifications": [
"cell line"
],
"institutional_certificates": [],
"modifications": [
{
"@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
"modality": "prime editing",
"status": "released"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0001253/",
"@type": [
"SampleTerm",
"OntologyTerm",
"Item"
],
"aliases": [
"igvf:THP-1"
],
"status": "released",
"summary": "THP-1",
"term_name": "THP-1"
}
],
"status": "released",
"summary": "Homo sapiens (male) THP-1 cell line (sorting details: FACS bin for 35-45% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library",
"taxa": "Homo sapiens"
}
],
"schema_version": "26",
"status": "released",
"submitted_by": {
"@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
"title": "Michael Montgomery"
},
"submitted_files_timestamp": "2023-12-05T18:31:08.707529+00:00",
"summary": "CRISPR prime editing Variant-EFFECTS sorted on the expression of PPIF integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
"targeted_genes": [
{
"@id": "/genes/ENSG00000108179/",
"geneid": "ENSG00000108179",
"name": "peptidylprolyl isomerase F",
"status": "released",
"symbol": "PPIF",
"synonyms": [
"PPIase F",
"peptidylprolyl isomerase F",
"Cyp-D",
"peptidyl-prolyl cis-trans isomerase F, mitochondrial",
"PPIF",
"CyP-M",
"cyclophilin F",
"peptidyl-prolyl cis-trans isomerase, mitochondrial",
"cyclophilin 3",
"cyclophilin D",
"rotamase F",
"mitochondrial cyclophilin",
"CypD",
"CYP3"
]
}
],
"uuid": "5b3d4708-c9de-46a0-878c-b77ad40531c9"
}