IGVFDS3561FSKT

released
{
    "@context": "/terms/",
    "@id": "/measurement-sets/IGVFDS3561FSKT/",
    "@type": [
        "MeasurementSet",
        "FileSet",
        "Item"
    ],
    "accession": "IGVFDS3561FSKT",
    "aliases": [
        "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep1-FFrep1-BinA_AmpliconSequencing"
    ],
    "assay_term": {
        "@id": "/assay-terms/OBI_0003661/",
        "status": "released",
        "term_name": "in vitro CRISPR screen using flow cytometry"
    },
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "missing sequencing kit",
                "detail": "Measurement set [IGVFDS3561FSKT](/measurement-sets/IGVFDS3561FSKT/) has sequence file(s) [IGVFFI0889MEKX](/sequence-files/IGVFFI0889MEKX/), [IGVFFI3790IBMZ](/sequence-files/IGVFFI3790IBMZ/), [IGVFFI5875UYYV](/sequence-files/IGVFFI5875UYYV/), [IGVFFI0022VIOD](/sequence-files/IGVFFI0022VIOD/), [IGVFFI8986LQYS](/sequence-files/IGVFFI8986LQYS/), [IGVFFI7638OJAS](/sequence-files/IGVFFI7638OJAS/), [IGVFFI0622ZAUE](/sequence-files/IGVFFI0622ZAUE/), [IGVFFI2801ZLNU](/sequence-files/IGVFFI2801ZLNU/), [IGVFFI0727ZNCW](/sequence-files/IGVFFI0727ZNCW/), [IGVFFI4017BJXN](/sequence-files/IGVFFI4017BJXN/), [IGVFFI9065PZZH](/sequence-files/IGVFFI9065PZZH/), [IGVFFI2116SCVI](/sequence-files/IGVFFI2116SCVI/), [IGVFFI2983HFEB](/sequence-files/IGVFFI2983HFEB/), [IGVFFI2234KVEY](/sequence-files/IGVFFI2234KVEY/), [IGVFFI5971ZSND](/sequence-files/IGVFFI5971ZSND/), [IGVFFI6396IJQM](/sequence-files/IGVFFI6396IJQM/), [IGVFFI0423OGGT](/sequence-files/IGVFFI0423OGGT/), [IGVFFI3851TWWR](/sequence-files/IGVFFI3851TWWR/), [IGVFFI8287PFCZ](/sequence-files/IGVFFI8287PFCZ/), [IGVFFI9646SAZO](/sequence-files/IGVFFI9646SAZO/), [IGVFFI1734CSWX](/sequence-files/IGVFFI1734CSWX/), [IGVFFI6051EHGV](/sequence-files/IGVFFI6051EHGV/), [IGVFFI7566GBAL](/sequence-files/IGVFFI7566GBAL/), [IGVFFI1448KYTZ](/sequence-files/IGVFFI1448KYTZ/), [IGVFFI2830OUDU](/sequence-files/IGVFFI2830OUDU/), [IGVFFI2613DASS](/sequence-files/IGVFFI2613DASS/), [IGVFFI0760BYPV](/sequence-files/IGVFFI0760BYPV/), [IGVFFI1166BZPS](/sequence-files/IGVFFI1166BZPS/), [IGVFFI4136FEKU](/sequence-files/IGVFFI4136FEKU/), [IGVFFI3658NCGQ](/sequence-files/IGVFFI3658NCGQ/), [IGVFFI3530LFPI](/sequence-files/IGVFFI3530LFPI/), [IGVFFI0588KREN](/sequence-files/IGVFFI0588KREN/), [IGVFFI4028RQVM](/sequence-files/IGVFFI4028RQVM/), [IGVFFI5768OQOS](/sequence-files/IGVFFI5768OQOS/), [IGVFFI6527UGIB](/sequence-files/IGVFFI6527UGIB/), [IGVFFI8051AMOU](/sequence-files/IGVFFI8051AMOU/), [IGVFFI3825QDEV](/sequence-files/IGVFFI3825QDEV/), [IGVFFI4462SQYP](/sequence-files/IGVFFI4462SQYP/), [IGVFFI5775YFXO](/sequence-files/IGVFFI5775YFXO/), [IGVFFI7273QMDX](/sequence-files/IGVFFI7273QMDX/), [IGVFFI3052URKY](/sequence-files/IGVFFI3052URKY/), [IGVFFI3399IAHG](/sequence-files/IGVFFI3399IAHG/), [IGVFFI8689QHOK](/sequence-files/IGVFFI8689QHOK/), [IGVFFI2552QGVT](/sequence-files/IGVFFI2552QGVT/), [IGVFFI2863GSZO](/sequence-files/IGVFFI2863GSZO/), [IGVFFI5902HDIW](/sequence-files/IGVFFI5902HDIW/), [IGVFFI1192CZMA](/sequence-files/IGVFFI1192CZMA/), [IGVFFI4068RZWC](/sequence-files/IGVFFI4068RZWC/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/measurement-sets/IGVFDS3561FSKT/"
            },
            {
                "category": "missing sequence specification",
                "detail": "Measurement set [IGVFDS3561FSKT](/measurement-sets/IGVFDS3561FSKT/) has sequence file(s): [IGVFFI0889MEKX](/sequence-files/IGVFFI0889MEKX/), [IGVFFI3790IBMZ](/sequence-files/IGVFFI3790IBMZ/), [IGVFFI5875UYYV](/sequence-files/IGVFFI5875UYYV/), [IGVFFI0022VIOD](/sequence-files/IGVFFI0022VIOD/), [IGVFFI8986LQYS](/sequence-files/IGVFFI8986LQYS/), [IGVFFI7638OJAS](/sequence-files/IGVFFI7638OJAS/), [IGVFFI0622ZAUE](/sequence-files/IGVFFI0622ZAUE/), [IGVFFI2801ZLNU](/sequence-files/IGVFFI2801ZLNU/), [IGVFFI0727ZNCW](/sequence-files/IGVFFI0727ZNCW/), [IGVFFI4017BJXN](/sequence-files/IGVFFI4017BJXN/), [IGVFFI9065PZZH](/sequence-files/IGVFFI9065PZZH/), [IGVFFI2116SCVI](/sequence-files/IGVFFI2116SCVI/), [IGVFFI2983HFEB](/sequence-files/IGVFFI2983HFEB/), [IGVFFI2234KVEY](/sequence-files/IGVFFI2234KVEY/), [IGVFFI5971ZSND](/sequence-files/IGVFFI5971ZSND/), [IGVFFI6396IJQM](/sequence-files/IGVFFI6396IJQM/), [IGVFFI0423OGGT](/sequence-files/IGVFFI0423OGGT/), [IGVFFI3851TWWR](/sequence-files/IGVFFI3851TWWR/), [IGVFFI8287PFCZ](/sequence-files/IGVFFI8287PFCZ/), [IGVFFI9646SAZO](/sequence-files/IGVFFI9646SAZO/), [IGVFFI1734CSWX](/sequence-files/IGVFFI1734CSWX/), [IGVFFI6051EHGV](/sequence-files/IGVFFI6051EHGV/), [IGVFFI7566GBAL](/sequence-files/IGVFFI7566GBAL/), [IGVFFI1448KYTZ](/sequence-files/IGVFFI1448KYTZ/), [IGVFFI2830OUDU](/sequence-files/IGVFFI2830OUDU/), [IGVFFI2613DASS](/sequence-files/IGVFFI2613DASS/), [IGVFFI0760BYPV](/sequence-files/IGVFFI0760BYPV/), [IGVFFI1166BZPS](/sequence-files/IGVFFI1166BZPS/), [IGVFFI4136FEKU](/sequence-files/IGVFFI4136FEKU/), [IGVFFI3658NCGQ](/sequence-files/IGVFFI3658NCGQ/), [IGVFFI3530LFPI](/sequence-files/IGVFFI3530LFPI/), [IGVFFI0588KREN](/sequence-files/IGVFFI0588KREN/), [IGVFFI4028RQVM](/sequence-files/IGVFFI4028RQVM/), [IGVFFI5768OQOS](/sequence-files/IGVFFI5768OQOS/), [IGVFFI6527UGIB](/sequence-files/IGVFFI6527UGIB/), [IGVFFI8051AMOU](/sequence-files/IGVFFI8051AMOU/), [IGVFFI3825QDEV](/sequence-files/IGVFFI3825QDEV/), [IGVFFI4462SQYP](/sequence-files/IGVFFI4462SQYP/), [IGVFFI5775YFXO](/sequence-files/IGVFFI5775YFXO/), [IGVFFI7273QMDX](/sequence-files/IGVFFI7273QMDX/), [IGVFFI3052URKY](/sequence-files/IGVFFI3052URKY/), [IGVFFI3399IAHG](/sequence-files/IGVFFI3399IAHG/), [IGVFFI8689QHOK](/sequence-files/IGVFFI8689QHOK/), [IGVFFI2552QGVT](/sequence-files/IGVFFI2552QGVT/), [IGVFFI2863GSZO](/sequence-files/IGVFFI2863GSZO/), [IGVFFI5902HDIW](/sequence-files/IGVFFI5902HDIW/), [IGVFFI1192CZMA](/sequence-files/IGVFFI1192CZMA/), [IGVFFI4068RZWC](/sequence-files/IGVFFI4068RZWC/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_missing_seqspec",
                "path": "/measurement-sets/IGVFDS3561FSKT/"
            },
            {
                "category": "mismatched status",
                "detail": "Released in vitro system [IGVFSM6493PXNX](/in-vitro-systems/IGVFSM6493PXNX/) has in progress subobject construct library set [IGVFDS2385DHZO](/construct-library-sets/IGVFDS2385DHZO/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/in-vitro-systems/IGVFSM6493PXNX/"
            }
        ],
        "WARNING": [
            {
                "category": "missing auxiliary set",
                "detail": "Measurement set [IGVFDS3561FSKT](/measurement-sets/IGVFDS3561FSKT/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_missing_auxiliary_set",
                "path": "/measurement-sets/IGVFDS3561FSKT/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization",
        "contact_pi": {
            "@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
            "title": "Jesse Engreitz"
        },
        "title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
    },
    "control_file_sets": [
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            "@id": "/measurement-sets/IGVFDS4869FXYY/",
            "accession": "IGVFDS4869FXYY",
            "aliases": [
                "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-Jurkat-PE2_Exp2_NegControl_AmpliconSequencing"
            ],
            "status": "released"
        }
    ],
    "control_for": [],
    "creation_timestamp": "2023-12-13T08:48:02.639900+00:00",
    "donors": [
        {
            "@id": "/human-donors/IGVFDO1080XFGV/",
            "accession": "IGVFDO1080XFGV",
            "aliases": [
                "igvf:donor_of_Jurkat"
            ],
            "sex": "male",
            "status": "released",
            "taxa": "Homo sapiens"
        }
    ],
    "externally_hosted": false,
    "file_set_type": "experimental data",
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            "@id": "/sequence-files/IGVFFI9065PZZH/",
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            "s3_uri": "s3://igvf-files/2023/12/13/dd477390-cd62-4ec2-9c02-c05993dbca5f/IGVFFI4068RZWC.fastq.gz",
            "sequencing_platform": {
                "@id": "/platform-terms/EFO_0008566/",
                "status": "released",
                "term_name": "Illumina NextSeq 550"
            },
            "status": "released",
            "submitted_file_name": "230423pPPIFEnformerJurkat-Jurkat-PE-pPPIF_Enformer-Amp-pPPIF-BioRep1-FFRep1_BinA-PCRrep5_S5_R2_001.fastq.gz",
            "upload_status": "validated"
        }
    ],
    "input_for": [
        "/analysis-sets/IGVFDS6299IJHG/"
    ],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set. This measurement set previously used Variant FlowFISH as a preferred_assay_title, but this enum is now removed. So it has been defaulted to Variant-EFFECTS.",
    "preferred_assay_title": "Variant-EFFECTS",
    "protocols": [
        "https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
    ],
    "release_timestamp": "2023-12-20T23:08:14.263327+00:00",
    "samples": [
        {
            "@id": "/in-vitro-systems/IGVFSM6493PXNX/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM6493PXNX",
            "aliases": [
                "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep1-FFrep1-BinA"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                {
                    "@id": "/construct-library-sets/IGVFDS2385DHZO/",
                    "accession": "IGVFDS2385DHZO",
                    "file_set_type": "guide library",
                    "status": "in progress",
                    "summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
                }
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0002796/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:Jurkat"
                    ],
                    "status": "released",
                    "summary": "Jurkat",
                    "term_name": "Jurkat"
                }
            ],
            "status": "released",
            "summary": "Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        }
    ],
    "schema_version": "23",
    "status": "released",
    "submitted_by": {
        "@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
        "title": "Michael Montgomery"
    },
    "submitted_files_timestamp": "2023-12-13T09:18:55.115702+00:00",
    "summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
    "targeted_genes": [
        {
            "@id": "/genes/ENSG00000108179/",
            "geneid": "ENSG00000108179",
            "name": "peptidylprolyl isomerase F",
            "status": "released",
            "symbol": "PPIF",
            "synonyms": [
                "rotamase F",
                "CYP3",
                "cyclophilin F",
                "mitochondrial cyclophilin",
                "peptidyl-prolyl cis-trans isomerase, mitochondrial",
                "CypD",
                "Cyp-D",
                "cyclophilin 3",
                "cyclophilin D",
                "CyP-M",
                "peptidyl-prolyl cis-trans isomerase F, mitochondrial",
                "PPIase F"
            ]
        }
    ],
    "uuid": "4ccd7492-29b5-4475-8921-861efcf80a2d"
}