IGVFDS3539CPFW

released

File Set Type

experimental data

Summary

CRISPR activation FACS screen sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting transcription start sites in IL2RA

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

IL2RA

Sample Summaries

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Protocols

Aliases

charles-gersbach:jurkats-measurement-set-crispra-vp64-il2ra-high-bin-rep2

Publications

Transcriptional and epigenetic regulators of human CD8+ T cell function identified through orthogonal CRISPR screens

Sequencing Results (Illumina)

1 item

Accession

File Format

Content Type

Illumina Read Type

Sequencing Run

Read Names

Associated seqspec Files

Sequencing Platform

Flowcell ID

Lane

Index

File Size

Lab

Upload Status

IGVFFI9895ZUGO

fastq

reads

Illumina MiSeq

000000000-JFKPJ

29 MB

Charles Gersbach, Duke

validated

Samples

1 item

Accession

Type

Sample Terms

Associated Construct Library Sets

Summary

IGVFSM2737ATVK

InVitroSystem

Jurkat

IGVFDS8183QSJO

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO2154QIFS

charles-gersbach:jurkat-tcells-donor-2

unspecified

Homo sapiens

Associated Construct Library Sets

1 item

Accession

Summary

Library Design Files

IGVFDS8183QSJO

guide (sgRNA) library targeting transcription start sites in IL2RA

IGVFFI3477BKFD

Control File Sets

1 item

Accession

Type

Summary

Aliases

Lab

IGVFDS0707NWZU

MeasurementSet

low FACS signal CRISPR activation FACS screen sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting transcription start sites in IL2RA