IGVFDS3508UNRE

released
{
    "@context": "/terms/",
    "@id": "/measurement-sets/IGVFDS3508UNRE/",
    "@type": [
        "MeasurementSet",
        "FileSet",
        "Item"
    ],
    "accession": "IGVFDS3508UNRE",
    "aliases": [
        "jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep2-FFrep1-BinA_AmpliconSequencing"
    ],
    "assay_term": {
        "@id": "/assay-terms/OBI_0003661/",
        "status": "released",
        "term_name": "in vitro CRISPR screen using flow cytometry"
    },
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "missing sequencing kit",
                "detail": "Measurement set [IGVFDS3508UNRE](/measurement-sets/IGVFDS3508UNRE/) has sequence file(s) [IGVFFI5220SEGT](/sequence-files/IGVFFI5220SEGT/), [IGVFFI2450FZLP](/sequence-files/IGVFFI2450FZLP/), [IGVFFI0270GJXZ](/sequence-files/IGVFFI0270GJXZ/), [IGVFFI4104OJOL](/sequence-files/IGVFFI4104OJOL/), [IGVFFI3927VITZ](/sequence-files/IGVFFI3927VITZ/), [IGVFFI5286AJLW](/sequence-files/IGVFFI5286AJLW/), [IGVFFI8225LGYT](/sequence-files/IGVFFI8225LGYT/), [IGVFFI0650VOCP](/sequence-files/IGVFFI0650VOCP/), [IGVFFI7658QWEX](/sequence-files/IGVFFI7658QWEX/), [IGVFFI9118SRIO](/sequence-files/IGVFFI9118SRIO/), [IGVFFI3822LIKN](/sequence-files/IGVFFI3822LIKN/), [IGVFFI6649BPZO](/sequence-files/IGVFFI6649BPZO/), [IGVFFI1380ZBOG](/sequence-files/IGVFFI1380ZBOG/), [IGVFFI0762JPEF](/sequence-files/IGVFFI0762JPEF/), [IGVFFI7140GPSE](/sequence-files/IGVFFI7140GPSE/), [IGVFFI5244PJVA](/sequence-files/IGVFFI5244PJVA/), [IGVFFI6600LZFN](/sequence-files/IGVFFI6600LZFN/), [IGVFFI0470VPWI](/sequence-files/IGVFFI0470VPWI/), [IGVFFI8105FAIG](/sequence-files/IGVFFI8105FAIG/), [IGVFFI9561FIEJ](/sequence-files/IGVFFI9561FIEJ/), [IGVFFI1594QVLZ](/sequence-files/IGVFFI1594QVLZ/), [IGVFFI6550HCLL](/sequence-files/IGVFFI6550HCLL/), [IGVFFI9423ASEJ](/sequence-files/IGVFFI9423ASEJ/), [IGVFFI4029PMIE](/sequence-files/IGVFFI4029PMIE/), [IGVFFI5939QOQB](/sequence-files/IGVFFI5939QOQB/), [IGVFFI9675ACTB](/sequence-files/IGVFFI9675ACTB/), [IGVFFI9505ELVP](/sequence-files/IGVFFI9505ELVP/), [IGVFFI5681GWAW](/sequence-files/IGVFFI5681GWAW/), [IGVFFI0829LSLR](/sequence-files/IGVFFI0829LSLR/), [IGVFFI8482NCCH](/sequence-files/IGVFFI8482NCCH/), [IGVFFI0260BCUD](/sequence-files/IGVFFI0260BCUD/), [IGVFFI8716NSCL](/sequence-files/IGVFFI8716NSCL/), [IGVFFI9012MGFS](/sequence-files/IGVFFI9012MGFS/), [IGVFFI3852HIBA](/sequence-files/IGVFFI3852HIBA/), [IGVFFI2094TQEM](/sequence-files/IGVFFI2094TQEM/), [IGVFFI1700ZPMK](/sequence-files/IGVFFI1700ZPMK/), [IGVFFI6585ASFH](/sequence-files/IGVFFI6585ASFH/), [IGVFFI9067FGUT](/sequence-files/IGVFFI9067FGUT/), [IGVFFI9541QRER](/sequence-files/IGVFFI9541QRER/), [IGVFFI7753XVXL](/sequence-files/IGVFFI7753XVXL/), [IGVFFI9212MMCW](/sequence-files/IGVFFI9212MMCW/), [IGVFFI6209VLSL](/sequence-files/IGVFFI6209VLSL/), [IGVFFI2720ERCN](/sequence-files/IGVFFI2720ERCN/), [IGVFFI7412OZMR](/sequence-files/IGVFFI7412OZMR/), [IGVFFI5859MEHD](/sequence-files/IGVFFI5859MEHD/), [IGVFFI5184BPUW](/sequence-files/IGVFFI5184BPUW/), [IGVFFI0278PMSR](/sequence-files/IGVFFI0278PMSR/), [IGVFFI9101FWZX](/sequence-files/IGVFFI9101FWZX/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/measurement-sets/IGVFDS3508UNRE/"
            },
            {
                "category": "missing sequence specification",
                "detail": "Measurement set [IGVFDS3508UNRE](/measurement-sets/IGVFDS3508UNRE/) has sequence file(s): [IGVFFI5220SEGT](/sequence-files/IGVFFI5220SEGT/), [IGVFFI2450FZLP](/sequence-files/IGVFFI2450FZLP/), [IGVFFI0270GJXZ](/sequence-files/IGVFFI0270GJXZ/), [IGVFFI4104OJOL](/sequence-files/IGVFFI4104OJOL/), [IGVFFI3927VITZ](/sequence-files/IGVFFI3927VITZ/), [IGVFFI5286AJLW](/sequence-files/IGVFFI5286AJLW/), [IGVFFI8225LGYT](/sequence-files/IGVFFI8225LGYT/), [IGVFFI0650VOCP](/sequence-files/IGVFFI0650VOCP/), [IGVFFI7658QWEX](/sequence-files/IGVFFI7658QWEX/), [IGVFFI9118SRIO](/sequence-files/IGVFFI9118SRIO/), [IGVFFI3822LIKN](/sequence-files/IGVFFI3822LIKN/), [IGVFFI6649BPZO](/sequence-files/IGVFFI6649BPZO/), [IGVFFI1380ZBOG](/sequence-files/IGVFFI1380ZBOG/), [IGVFFI0762JPEF](/sequence-files/IGVFFI0762JPEF/), [IGVFFI7140GPSE](/sequence-files/IGVFFI7140GPSE/), [IGVFFI5244PJVA](/sequence-files/IGVFFI5244PJVA/), [IGVFFI6600LZFN](/sequence-files/IGVFFI6600LZFN/), [IGVFFI0470VPWI](/sequence-files/IGVFFI0470VPWI/), [IGVFFI8105FAIG](/sequence-files/IGVFFI8105FAIG/), [IGVFFI9561FIEJ](/sequence-files/IGVFFI9561FIEJ/), [IGVFFI1594QVLZ](/sequence-files/IGVFFI1594QVLZ/), [IGVFFI6550HCLL](/sequence-files/IGVFFI6550HCLL/), [IGVFFI9423ASEJ](/sequence-files/IGVFFI9423ASEJ/), [IGVFFI4029PMIE](/sequence-files/IGVFFI4029PMIE/), [IGVFFI5939QOQB](/sequence-files/IGVFFI5939QOQB/), [IGVFFI9675ACTB](/sequence-files/IGVFFI9675ACTB/), [IGVFFI9505ELVP](/sequence-files/IGVFFI9505ELVP/), [IGVFFI5681GWAW](/sequence-files/IGVFFI5681GWAW/), [IGVFFI0829LSLR](/sequence-files/IGVFFI0829LSLR/), [IGVFFI8482NCCH](/sequence-files/IGVFFI8482NCCH/), [IGVFFI0260BCUD](/sequence-files/IGVFFI0260BCUD/), [IGVFFI8716NSCL](/sequence-files/IGVFFI8716NSCL/), [IGVFFI9012MGFS](/sequence-files/IGVFFI9012MGFS/), [IGVFFI3852HIBA](/sequence-files/IGVFFI3852HIBA/), [IGVFFI2094TQEM](/sequence-files/IGVFFI2094TQEM/), [IGVFFI1700ZPMK](/sequence-files/IGVFFI1700ZPMK/), [IGVFFI6585ASFH](/sequence-files/IGVFFI6585ASFH/), [IGVFFI9067FGUT](/sequence-files/IGVFFI9067FGUT/), [IGVFFI9541QRER](/sequence-files/IGVFFI9541QRER/), [IGVFFI7753XVXL](/sequence-files/IGVFFI7753XVXL/), [IGVFFI9212MMCW](/sequence-files/IGVFFI9212MMCW/), [IGVFFI6209VLSL](/sequence-files/IGVFFI6209VLSL/), [IGVFFI2720ERCN](/sequence-files/IGVFFI2720ERCN/), [IGVFFI7412OZMR](/sequence-files/IGVFFI7412OZMR/), [IGVFFI5859MEHD](/sequence-files/IGVFFI5859MEHD/), [IGVFFI5184BPUW](/sequence-files/IGVFFI5184BPUW/), [IGVFFI0278PMSR](/sequence-files/IGVFFI0278PMSR/), [IGVFFI9101FWZX](/sequence-files/IGVFFI9101FWZX/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_missing_seqspec",
                "path": "/measurement-sets/IGVFDS3508UNRE/"
            },
            {
                "category": "mismatched status",
                "detail": "Released in vitro system [IGVFSM1225BLTJ](/in-vitro-systems/IGVFSM1225BLTJ/) has in progress subobject construct library set [IGVFDS2385DHZO](/construct-library-sets/IGVFDS2385DHZO/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/in-vitro-systems/IGVFSM1225BLTJ/"
            }
        ],
        "WARNING": [
            {
                "category": "missing auxiliary set",
                "detail": "Measurement set [IGVFDS3508UNRE](/measurement-sets/IGVFDS3508UNRE/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_missing_auxiliary_set",
                "path": "/measurement-sets/IGVFDS3508UNRE/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization",
        "contact_pi": {
            "@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
            "title": "Jesse Engreitz"
        },
        "title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
    },
    "control_file_sets": [
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            "@id": "/measurement-sets/IGVFDS5128CXIS/",
            "accession": "IGVFDS5128CXIS",
            "aliases": [
                "jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-THP1-PE2_Exp4_NegControl_AmpliconSequencing"
            ],
            "status": "released"
        }
    ],
    "control_for": [],
    "creation_timestamp": "2023-12-13T08:01:43.286146+00:00",
    "donors": [
        {
            "@id": "/human-donors/IGVFDO7359LVPI/",
            "accession": "IGVFDO7359LVPI",
            "aliases": [
                "igvf:donor_of_THP1"
            ],
            "sex": "male",
            "status": "released",
            "taxa": "Homo sapiens"
        }
    ],
    "externally_hosted": false,
    "file_set_type": "experimental data",
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            "@id": "/sequence-files/IGVFFI5220SEGT/",
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        },
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                "jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep2-FFrep1-BinA-PCRrep3_I2"
            ],
            "content_type": "reads",
            "controlled_access": false,
            "creation_timestamp": "2023-12-13T16:37:23.452494+00:00",
            "file_format": "fastq",
            "file_size": 2205430,
            "href": "/sequence-files/IGVFFI9101FWZX/@@download/IGVFFI9101FWZX.fastq.gz",
            "s3_uri": "s3://igvf-files/2023/12/13/8c62ee66-4609-4cb6-bbd2-c4d49689b734/IGVFFI9101FWZX.fastq.gz",
            "sequencing_platform": {
                "@id": "/platform-terms/EFO_0008566/",
                "status": "released",
                "term_name": "Illumina NextSeq 550"
            },
            "status": "released",
            "submitted_file_name": "230523pPPIFEnformerTHP1-THP1-PE-pPPIF_Enformer-Amp-pPPIF-BioRep2-FFRep1_BinA-PCRrep3_S195_I2_001.fastq.gz",
            "upload_status": "validated"
        }
    ],
    "input_for": [
        "/analysis-sets/IGVFDS4359OODY/"
    ],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set. This measurement set previously used Variant FlowFISH as a preferred_assay_title, but this enum is now removed. So it has been defaulted to Variant-EFFECTS.",
    "preferred_assay_title": "Variant-EFFECTS",
    "protocols": [
        "https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
    ],
    "release_timestamp": "2023-12-20T23:07:50.852420+00:00",
    "samples": [
        {
            "@id": "/in-vitro-systems/IGVFSM1225BLTJ/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM1225BLTJ",
            "aliases": [
                "jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep2-FFrep1-BinA"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                {
                    "@id": "/construct-library-sets/IGVFDS2385DHZO/",
                    "accession": "IGVFDS2385DHZO",
                    "file_set_type": "guide library",
                    "status": "in progress",
                    "summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
                }
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0001253/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:THP-1"
                    ],
                    "status": "released",
                    "summary": "THP-1",
                    "term_name": "THP-1"
                }
            ],
            "status": "released",
            "summary": "THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        }
    ],
    "schema_version": "23",
    "status": "released",
    "submitted_by": {
        "@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
        "title": "Michael Montgomery"
    },
    "submitted_files_timestamp": "2023-12-13T08:50:20.067891+00:00",
    "summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
    "targeted_genes": [
        {
            "@id": "/genes/ENSG00000108179/",
            "geneid": "ENSG00000108179",
            "name": "peptidylprolyl isomerase F",
            "status": "released",
            "symbol": "PPIF",
            "synonyms": [
                "rotamase F",
                "CYP3",
                "cyclophilin F",
                "mitochondrial cyclophilin",
                "peptidyl-prolyl cis-trans isomerase, mitochondrial",
                "CypD",
                "Cyp-D",
                "cyclophilin 3",
                "cyclophilin D",
                "CyP-M",
                "peptidyl-prolyl cis-trans isomerase F, mitochondrial",
                "PPIase F"
            ]
        }
    ],
    "uuid": "766fa2c0-becc-4e7c-b726-be511e9ce86d"
}