IGVFDS3328PNPV

released
File Set Type
experimental data
Summary
mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Assay Term
Targeted Genes
Sample Summaries
  • HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
Protocols
Aliases
jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF2-BinD-PCR1_S64
jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF2-BinD-PCR2_S70
jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF2-BinD-PCR3_S160
jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF2-BinD-PCR4_S166

Sequencing Results

4 items
fastq
reads
1
TGCTGCTG:CTCT
9.2 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TGCTGCTG:ATTG
14 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
CTATGCGT:ATTG
14 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
CTATGCGT:CTCT
8.9 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Auxiliary Datasets

1 item
Accession
Type
Summary
Files
Aliases
Lab
AuxiliarySet
cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 3 more measurement sets
  • jesse-engreitz:auxiliary_sortparams_CRUDO_KITLG-Auxin6hrs-FF2
Jesse Engreitz, Stanford

Documents

1 item
Page
Description
Type
Download
Preview
overview of experimental design
experimental protocol

Attribution