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IGVFDS3215BSOP

released
File Set Type
experimental data
Summary
mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Assay Term
in vitro CRISPR screen using flow cytometry
Targeted Genes
Sample Summaries
  • HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
Protocols
Aliases
jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF4-BinE-PCR1_S41
jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF4-BinE-PCR2_S47
jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF4-BinE-PCR3_S137
jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF4-BinE-PCR4_S143

Sequencing Results

4 items
fastq
reads
1
TCCTTGGT:CTCT
9.5 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TCCTTGGT:ATTG
16 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
GAACCTAG:ATTG
15 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
GAACCTAG:CTCT
6.8 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Auxiliary Datasets

1 item
Accession
Type
Summary
Files
Aliases
Lab
AuxiliarySet
cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets
  • jesse-engreitz:auxiliary_sortparams_CRUDO_KITLG-Auxin0hrs-FF4
Jesse Engreitz, Stanford

Documents

1 item
Page
Description
Type
Download
Preview
overview of experimental design
experimental protocol

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford