IGVFDS3202JLFH

{
    "@context": "/terms/",
    "@id": "/measurement-sets/IGVFDS3202JLFH/",
    "@type": [
        "MeasurementSet",
        "FileSet",
        "Item"
    ],
    "accession": "IGVFDS3202JLFH",
    "aliases": [
        "jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep3-BinB_AmpliconSequencing"
    ],
    "assay_term": {
        "@id": "/assay-terms/OBI_0003661/",
        "status": "released",
        "term_name": "in vitro CRISPR screen using flow cytometry"
    },
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "missing sequencing kit",
                "detail": "Measurement set [IGVFDS3202JLFH](/measurement-sets/IGVFDS3202JLFH/) has sequence file(s) [IGVFFI6538ZMEO](/sequence-files/IGVFFI6538ZMEO/), [IGVFFI0701RIOV](/sequence-files/IGVFFI0701RIOV/), [IGVFFI7676WXBD](/sequence-files/IGVFFI7676WXBD/), [IGVFFI7601XPKB](/sequence-files/IGVFFI7601XPKB/), [IGVFFI2514NNAS](/sequence-files/IGVFFI2514NNAS/), [IGVFFI9018COQC](/sequence-files/IGVFFI9018COQC/), [IGVFFI7233GBWO](/sequence-files/IGVFFI7233GBWO/), [IGVFFI1030PUMH](/sequence-files/IGVFFI1030PUMH/), [IGVFFI6767EEDH](/sequence-files/IGVFFI6767EEDH/), [IGVFFI0194LBLW](/sequence-files/IGVFFI0194LBLW/), [IGVFFI8750EXGU](/sequence-files/IGVFFI8750EXGU/), [IGVFFI0615OFGZ](/sequence-files/IGVFFI0615OFGZ/), [IGVFFI2177KIRQ](/sequence-files/IGVFFI2177KIRQ/), [IGVFFI8574QAWV](/sequence-files/IGVFFI8574QAWV/), [IGVFFI8736ONYO](/sequence-files/IGVFFI8736ONYO/), [IGVFFI8120NISF](/sequence-files/IGVFFI8120NISF/), [IGVFFI0224MFEA](/sequence-files/IGVFFI0224MFEA/), [IGVFFI2083MTBF](/sequence-files/IGVFFI2083MTBF/), [IGVFFI3582YPET](/sequence-files/IGVFFI3582YPET/), [IGVFFI5648PKTT](/sequence-files/IGVFFI5648PKTT/), [IGVFFI1153PKCI](/sequence-files/IGVFFI1153PKCI/), [IGVFFI2526TPZK](/sequence-files/IGVFFI2526TPZK/), [IGVFFI4236BGOI](/sequence-files/IGVFFI4236BGOI/), [IGVFFI9616HUUZ](/sequence-files/IGVFFI9616HUUZ/), [IGVFFI8776DOMF](/sequence-files/IGVFFI8776DOMF/), [IGVFFI9335PTDU](/sequence-files/IGVFFI9335PTDU/), [IGVFFI2233NCAF](/sequence-files/IGVFFI2233NCAF/), [IGVFFI4378UYHG](/sequence-files/IGVFFI4378UYHG/), [IGVFFI3450OUFP](/sequence-files/IGVFFI3450OUFP/), [IGVFFI8966DVQL](/sequence-files/IGVFFI8966DVQL/), [IGVFFI9627AMQK](/sequence-files/IGVFFI9627AMQK/), [IGVFFI2590SIXT](/sequence-files/IGVFFI2590SIXT/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/measurement-sets/IGVFDS3202JLFH/"
            },
            {
                "category": "missing sequence specification",
                "detail": "Measurement set [IGVFDS3202JLFH](/measurement-sets/IGVFDS3202JLFH/) has sequence file(s): [IGVFFI6538ZMEO](/sequence-files/IGVFFI6538ZMEO/), [IGVFFI0701RIOV](/sequence-files/IGVFFI0701RIOV/), [IGVFFI7676WXBD](/sequence-files/IGVFFI7676WXBD/), [IGVFFI7601XPKB](/sequence-files/IGVFFI7601XPKB/), [IGVFFI2514NNAS](/sequence-files/IGVFFI2514NNAS/), [IGVFFI9018COQC](/sequence-files/IGVFFI9018COQC/), [IGVFFI7233GBWO](/sequence-files/IGVFFI7233GBWO/), [IGVFFI1030PUMH](/sequence-files/IGVFFI1030PUMH/), [IGVFFI6767EEDH](/sequence-files/IGVFFI6767EEDH/), [IGVFFI0194LBLW](/sequence-files/IGVFFI0194LBLW/), [IGVFFI8750EXGU](/sequence-files/IGVFFI8750EXGU/), [IGVFFI0615OFGZ](/sequence-files/IGVFFI0615OFGZ/), [IGVFFI2177KIRQ](/sequence-files/IGVFFI2177KIRQ/), [IGVFFI8574QAWV](/sequence-files/IGVFFI8574QAWV/), [IGVFFI8736ONYO](/sequence-files/IGVFFI8736ONYO/), [IGVFFI8120NISF](/sequence-files/IGVFFI8120NISF/), [IGVFFI0224MFEA](/sequence-files/IGVFFI0224MFEA/), [IGVFFI2083MTBF](/sequence-files/IGVFFI2083MTBF/), [IGVFFI3582YPET](/sequence-files/IGVFFI3582YPET/), [IGVFFI5648PKTT](/sequence-files/IGVFFI5648PKTT/), [IGVFFI1153PKCI](/sequence-files/IGVFFI1153PKCI/), [IGVFFI2526TPZK](/sequence-files/IGVFFI2526TPZK/), [IGVFFI4236BGOI](/sequence-files/IGVFFI4236BGOI/), [IGVFFI9616HUUZ](/sequence-files/IGVFFI9616HUUZ/), [IGVFFI8776DOMF](/sequence-files/IGVFFI8776DOMF/), [IGVFFI9335PTDU](/sequence-files/IGVFFI9335PTDU/), [IGVFFI2233NCAF](/sequence-files/IGVFFI2233NCAF/), [IGVFFI4378UYHG](/sequence-files/IGVFFI4378UYHG/), [IGVFFI3450OUFP](/sequence-files/IGVFFI3450OUFP/), [IGVFFI8966DVQL](/sequence-files/IGVFFI8966DVQL/), [IGVFFI9627AMQK](/sequence-files/IGVFFI9627AMQK/), [IGVFFI2590SIXT](/sequence-files/IGVFFI2590SIXT/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_missing_seqspec",
                "path": "/measurement-sets/IGVFDS3202JLFH/"
            },
            {
                "category": "mismatched status",
                "detail": "Preview in vitro system [IGVFSM4883HGPU](/in-vitro-systems/IGVFSM4883HGPU/) has in progress subobject treatment [cf87f410-54ee-48ef-b654-8443c4fee032](/treatments/cf87f410-54ee-48ef-b654-8443c4fee032/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/in-vitro-systems/IGVFSM4883HGPU/"
            },
            {
                "category": "mismatched status",
                "detail": "Preview in vitro system [IGVFSM4883HGPU](/in-vitro-systems/IGVFSM4883HGPU/) has in progress subobject treatment [4b5f0d13-ec20-4b91-85f5-a009cd91451f](/treatments/4b5f0d13-ec20-4b91-85f5-a009cd91451f/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/in-vitro-systems/IGVFSM4883HGPU/"
            }
        ],
        "WARNING": [
            {
                "category": "missing auxiliary set",
                "detail": "Measurement set [IGVFDS3202JLFH](/measurement-sets/IGVFDS3202JLFH/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_missing_auxiliary_set",
                "path": "/measurement-sets/IGVFDS3202JLFH/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization",
        "contact_pi": {
            "@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
            "title": "Jesse Engreitz"
        },
        "title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
    },
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        {
            "@id": "/measurement-sets/IGVFDS6874YIXA/",
            "accession": "IGVFDS6874YIXA",
            "aliases": [
                "jesse-engreitz:Jurkat_Stim-IL2RA_prom-NegControl_AmpliconSequencing"
            ],
            "status": "preview"
        }
    ],
    "control_for": [],
    "creation_timestamp": "2024-11-22T00:47:46.867926+00:00",
    "donors": [
        {
            "@id": "/human-donors/IGVFDO1080XFGV/",
            "accession": "IGVFDO1080XFGV",
            "aliases": [
                "igvf:donor_of_Jurkat"
            ],
            "sex": "male",
            "status": "released",
            "taxa": "Homo sapiens"
        }
    ],
    "externally_hosted": false,
    "file_set_type": "experimental data",
    "files": [
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            "@id": "/sequence-files/IGVFFI6538ZMEO/",
            "accession": "IGVFFI6538ZMEO",
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                "term_name": "Illumina NovaSeq X"
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            "upload_status": "validated"
        },
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            "@id": "/sequence-files/IGVFFI0615OFGZ/",
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            "@id": "/sequence-files/IGVFFI2177KIRQ/",
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        "title": "Jesse Engreitz, Stanford"
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    "notes": "This measurement set previously used Variant FlowFISH as a preferred_assay_title, but this enum is now removed. So it has been defaulted to Variant-EFFECTS.",
    "preferred_assay_title": "Variant-EFFECTS",
    "protocols": [
        "https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
    ],
    "samples": [
        {
            "@id": "/in-vitro-systems/IGVFSM4883HGPU/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM4883HGPU",
            "aliases": [
                "jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep3-BinB"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                {
                    "@id": "/construct-library-sets/IGVFDS6128KVUP/",
                    "accession": "IGVFDS6128KVUP",
                    "file_set_type": "guide library",
                    "status": "preview",
                    "summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
                }
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0002796/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:Jurkat"
                    ],
                    "status": "released",
                    "summary": "Jurkat",
                    "term_name": "Jurkat"
                }
            ],
            "status": "preview",
            "summary": "Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 26-50% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens",
            "treatments": [
                {
                    "@id": "/treatments/4b5f0d13-ec20-4b91-85f5-a009cd91451f/",
                    "status": "in progress",
                    "treatment_term_name": "phorbol 13-acetate 12-myristate"
                },
                {
                    "@id": "/treatments/cf87f410-54ee-48ef-b654-8443c4fee032/",
                    "status": "in progress",
                    "treatment_term_name": "T-cell surface glycoprotein CD3 gamma chain"
                }
            ]
        }
    ],
    "schema_version": "23",
    "status": "preview",
    "submitted_by": {
        "@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
        "title": "Michael Montgomery"
    },
    "submitted_files_timestamp": "2024-11-22T06:03:10.138818+00:00",
    "summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
    "targeted_genes": [
        {
            "@id": "/genes/ENSG00000134460/",
            "geneid": "ENSG00000134460",
            "name": "interleukin 2 receptor subunit alpha",
            "status": "released",
            "symbol": "IL2RA",
            "synonyms": [
                "interleukin-2 receptor subunit alpha",
                "IL2R",
                "TAC antigen",
                "IMD41",
                "IL-2R subunit alpha",
                "CD25",
                "IL-2 receptor subunit alpha",
                "IDDM10",
                "TCGFR",
                "p55",
                "interleukin 2 receptor, alpha"
            ]
        }
    ],
    "uuid": "0667f8ab-182a-4549-b8e2-b4e91eb052ad"
}