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MeasurementSet
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IGVFDS3198NMHN
IGVFDS3198NMHN
released
Json view
File Set Type
experimental data
Summary
knockout RNA-seq integrating a guide (sgRNA) library targeting transcription start sites in ZNF217
Assay Term
bulk RNA-seq assay
Sample Summaries
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
Construct Library Sets
IGVFDS7189YPQQ
Guide (sgRNA) library targeting transcription start sites in ZNF217
Protocols
https://www.protocols.io/view/tfome-crisprko-screens-81wgbxk8olpk/v1
https://www.protocols.io/view/rnaseq-of-primary-human-t-cells-overexpressing-bat-c5pdy5i6
Aliases
charles-gersbach:cd8-tcells-rna-seq-cas-ko-znf217-rep3
Publications
Transcriptional and epigenetic regulators of human CD8+ T cell function identified through orthogonal CRISPR screens
Sequencing Results (Illumina)
2 items
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Associated seqspec Files
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI0834TQEW
fastq
reads
R1
3
Illumina NovaSeq 6000
1
2.4 GB
Charles Gersbach, Duke
validated
IGVFFI3623WAYN
fastq
reads
R2
3
Illumina NovaSeq 6000
1
2.6 GB
Charles Gersbach, Duke
validated
Samples
Donors
File Sets Using This Measurement Set as an Input
Attribution
Award
HG012053
Principal Investigator(s)
Charles Gersbach
,
Gregory Crawford
,
Tim Reddy
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke