IGVFDS3143OSQG

released

File Set Type

experimental data

Summary

CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

KITLG

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of KITLG) modified with a guide library

Construct Library Sets

IGVFDS7846UISG Guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinC-PCR1_S27

jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinC-PCR2_S33

jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinC-PCR3_S123

jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinC-PCR4_S129

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM0811AZWF

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of KITLG) modified with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS0329USVJ

AuxiliarySet

cell sorting for CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI6162WULK