IGVFDS2690TTFH

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

MYC

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Construct Library Sets

IGVFDS7857SYCK guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR1_Rep1_S114

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR1_Rep2_S169

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR2_Rep1_S120

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR2_Rep2_S175

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR3_Rep1_S126

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR3_Rep2_S181

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR4_Rep1_S132

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR4_Rep2_S187

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM0373CYTT

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS4894APIT

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI6767UOHJ, IGVFFI1838TDNU, IGVFFI5888TLGJ, IGVFFI4501LVHP, IGVFFI4677UICJ, IGVFFI1659OMPY, IGVFFI1400XMFH, IGVFFI7202ZYZL

jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR1_Rep1_S114
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR1_Rep2_S169
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR2_Rep1_S120
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR2_Rep2_S175
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR3_Rep1_S126
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR3_Rep2_S181
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR4_Rep1_S132
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinD-PCR4_Rep2_S187

Jesse Engreitz, Stanford

IGVFDS9055BZJI

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI6885TVYB