{
"@context": "/terms/",
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"FileSet",
"Item"
],
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],
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"status": "released",
"term_name": "in vitro CRISPR screen using single-cell RNA-seq"
},
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{
"category": "missing sequence specification",
"detail": "Auxiliary set [IGVFDS4697CFEN](/auxiliary-sets/IGVFDS4697CFEN/) has sequence file(s): [IGVFFI5894VLNQ](/sequence-files/IGVFFI5894VLNQ/), [IGVFFI2943FXTE](/sequence-files/IGVFFI2943FXTE/), [IGVFFI0738KGIZ](/sequence-files/IGVFFI0738KGIZ/), [IGVFFI8137NKZA](/sequence-files/IGVFFI8137NKZA/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
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},
{
"category": "NTR term ID",
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"name": "audit_ntr_term_id",
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},
{
"category": "missing sequence specification",
"detail": "Measurement set [IGVFDS2630GWQK](/measurement-sets/IGVFDS2630GWQK/) has sequence file(s): [IGVFFI3938RSXN](/sequence-files/IGVFFI3938RSXN/), [IGVFFI4067OVRO](/sequence-files/IGVFFI4067OVRO/), [IGVFFI6041RJGC](/sequence-files/IGVFFI6041RJGC/), [IGVFFI3742YSMD](/sequence-files/IGVFFI3742YSMD/), [IGVFFI8627TVTI](/sequence-files/IGVFFI8627TVTI/), [IGVFFI0620TEXL](/sequence-files/IGVFFI0620TEXL/), [IGVFFI0976WHCB](/sequence-files/IGVFFI0976WHCB/), [IGVFFI0196UOBL](/sequence-files/IGVFFI0196UOBL/), [IGVFFI2019MGNR](/sequence-files/IGVFFI2019MGNR/), [IGVFFI1078PNYM](/sequence-files/IGVFFI1078PNYM/), [IGVFFI3713VLLN](/sequence-files/IGVFFI3713VLLN/), [IGVFFI2963CBJA](/sequence-files/IGVFFI2963CBJA/), [IGVFFI5903BYTZ](/sequence-files/IGVFFI5903BYTZ/), [IGVFFI7742JDLT](/sequence-files/IGVFFI7742JDLT/), [IGVFFI4291WETA](/sequence-files/IGVFFI4291WETA/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_missing_seqspec",
"path": "/measurement-sets/IGVFDS2630GWQK/"
}
]
},
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],
"input_for": [
"/analysis-sets/IGVFDS7943IIWZ/"
],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"notes": "The readout 86015c12-b9a6-43ba-9601-5d75aab4d39c was removed from this measurement set.",
"preferred_assay_title": "Perturb-seq",
"protocols": [
"https://www.protocols.io/view/systematic-perturb-seq-to-discover-endothelial-cel-261ged73yv47/v1"
],
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM3814HZQI/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM3814HZQI",
"aliases": [
"jesse-engreitz:in_vitro_systems-scRNAseq_2kG_11AMDox_6"
],
"classifications": [
"cell line"
],
"construct_library_sets": [
{
"@id": "/construct-library-sets/IGVFDS1298TUYG/",
"accession": "IGVFDS1298TUYG",
"file_set_type": "guide library",
"status": "preview",
"summary": "guide (sgRNA) library targeting genes in many genomic loci"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"status": "released"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"@type": [
"SampleTerm",
"OntologyTerm",
"Item"
],
"aliases": [
"igvf:TeloHAEC"
],
"status": "released",
"summary": "TeloHAEC",
"term_name": "TeloHAEC"
}
],
"status": "preview",
"summary": "TeloHAEC cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library",
"taxa": "Homo sapiens"
}
],
"schema_version": "23",
"status": "preview",
"submitted_by": {
"@id": "/users/05fa0dc9-0e33-427c-9d1f-82393edc4a03/",
"title": "Helen Kang"
},
"submitted_files_timestamp": "2023-12-20T22:32:05.333190+00:00",
"summary": "CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci",
"uuid": "e611ae5c-0e45-4465-8e2b-f877be450eb5"
}