{
"@context": "/terms/",
"@id": "/measurement-sets/IGVFDS2584QAOI/",
"@type": [
"MeasurementSet",
"FileSet",
"Item"
],
"accession": "IGVFDS2584QAOI",
"aliases": [
"jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep2-FFRep2-BinC_AmpliconSequencing"
],
"assay_term": {
"@id": "/assay-terms/OBI_0003661/",
"status": "released",
"term_name": "in vitro CRISPR screen using flow cytometry"
},
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Preview in vitro system [IGVFSM9894RFCK](/in-vitro-systems/IGVFSM9894RFCK/) has in progress subobject treatment [cf87f410-54ee-48ef-b654-8443c4fee032](/treatments/cf87f410-54ee-48ef-b654-8443c4fee032/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/in-vitro-systems/IGVFSM9894RFCK/"
},
{
"category": "mismatched status",
"detail": "Preview in vitro system [IGVFSM9894RFCK](/in-vitro-systems/IGVFSM9894RFCK/) has in progress subobject treatment [4b5f0d13-ec20-4b91-85f5-a009cd91451f](/treatments/4b5f0d13-ec20-4b91-85f5-a009cd91451f/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/in-vitro-systems/IGVFSM9894RFCK/"
},
{
"category": "missing sequencing kit",
"detail": "Measurement set [IGVFDS2584QAOI](/measurement-sets/IGVFDS2584QAOI/) has sequence file(s) [IGVFFI8044PCEV](/sequence-files/IGVFFI8044PCEV/), [IGVFFI5367QWQD](/sequence-files/IGVFFI5367QWQD/), [IGVFFI7920RECR](/sequence-files/IGVFFI7920RECR/), [IGVFFI9988HXLV](/sequence-files/IGVFFI9988HXLV/), [IGVFFI4514AALO](/sequence-files/IGVFFI4514AALO/), [IGVFFI8242XCZW](/sequence-files/IGVFFI8242XCZW/), [IGVFFI3614NCDO](/sequence-files/IGVFFI3614NCDO/), [IGVFFI9780TQCC](/sequence-files/IGVFFI9780TQCC/), [IGVFFI4406KJVX](/sequence-files/IGVFFI4406KJVX/), [IGVFFI3394MFDZ](/sequence-files/IGVFFI3394MFDZ/), [IGVFFI7425OVAH](/sequence-files/IGVFFI7425OVAH/), [IGVFFI1492PFKC](/sequence-files/IGVFFI1492PFKC/), [IGVFFI7043YCIT](/sequence-files/IGVFFI7043YCIT/), [IGVFFI3970DFOH](/sequence-files/IGVFFI3970DFOH/), [IGVFFI2372YRAK](/sequence-files/IGVFFI2372YRAK/), [IGVFFI7031EUDE](/sequence-files/IGVFFI7031EUDE/), [IGVFFI7098MLCG](/sequence-files/IGVFFI7098MLCG/), [IGVFFI0761XQIU](/sequence-files/IGVFFI0761XQIU/), [IGVFFI6915XJGX](/sequence-files/IGVFFI6915XJGX/), [IGVFFI7268MIJU](/sequence-files/IGVFFI7268MIJU/), [IGVFFI1739ESWZ](/sequence-files/IGVFFI1739ESWZ/), [IGVFFI7221OEAW](/sequence-files/IGVFFI7221OEAW/), [IGVFFI9985LYEB](/sequence-files/IGVFFI9985LYEB/), [IGVFFI5652HXMG](/sequence-files/IGVFFI5652HXMG/), [IGVFFI0687EJYU](/sequence-files/IGVFFI0687EJYU/), [IGVFFI7116WYMJ](/sequence-files/IGVFFI7116WYMJ/), [IGVFFI9907BOPH](/sequence-files/IGVFFI9907BOPH/), [IGVFFI1994WQKG](/sequence-files/IGVFFI1994WQKG/), [IGVFFI5903FIOU](/sequence-files/IGVFFI5903FIOU/), [IGVFFI5818TRBA](/sequence-files/IGVFFI5818TRBA/), [IGVFFI8372TZIV](/sequence-files/IGVFFI8372TZIV/), [IGVFFI2138IGZI](/sequence-files/IGVFFI2138IGZI/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_inconsistent_sequencing_kit",
"path": "/measurement-sets/IGVFDS2584QAOI/"
},
{
"category": "missing sequence specification",
"detail": "Measurement set [IGVFDS2584QAOI](/measurement-sets/IGVFDS2584QAOI/) has sequence file(s): [IGVFFI8044PCEV](/sequence-files/IGVFFI8044PCEV/), [IGVFFI5367QWQD](/sequence-files/IGVFFI5367QWQD/), [IGVFFI7920RECR](/sequence-files/IGVFFI7920RECR/), [IGVFFI9988HXLV](/sequence-files/IGVFFI9988HXLV/), [IGVFFI4514AALO](/sequence-files/IGVFFI4514AALO/), [IGVFFI8242XCZW](/sequence-files/IGVFFI8242XCZW/), [IGVFFI3614NCDO](/sequence-files/IGVFFI3614NCDO/), [IGVFFI9780TQCC](/sequence-files/IGVFFI9780TQCC/), [IGVFFI4406KJVX](/sequence-files/IGVFFI4406KJVX/), [IGVFFI3394MFDZ](/sequence-files/IGVFFI3394MFDZ/), [IGVFFI7425OVAH](/sequence-files/IGVFFI7425OVAH/), [IGVFFI1492PFKC](/sequence-files/IGVFFI1492PFKC/), [IGVFFI7043YCIT](/sequence-files/IGVFFI7043YCIT/), [IGVFFI3970DFOH](/sequence-files/IGVFFI3970DFOH/), [IGVFFI2372YRAK](/sequence-files/IGVFFI2372YRAK/), [IGVFFI7031EUDE](/sequence-files/IGVFFI7031EUDE/), [IGVFFI7098MLCG](/sequence-files/IGVFFI7098MLCG/), [IGVFFI0761XQIU](/sequence-files/IGVFFI0761XQIU/), [IGVFFI6915XJGX](/sequence-files/IGVFFI6915XJGX/), [IGVFFI7268MIJU](/sequence-files/IGVFFI7268MIJU/), [IGVFFI1739ESWZ](/sequence-files/IGVFFI1739ESWZ/), [IGVFFI7221OEAW](/sequence-files/IGVFFI7221OEAW/), [IGVFFI9985LYEB](/sequence-files/IGVFFI9985LYEB/), [IGVFFI5652HXMG](/sequence-files/IGVFFI5652HXMG/), [IGVFFI0687EJYU](/sequence-files/IGVFFI0687EJYU/), [IGVFFI7116WYMJ](/sequence-files/IGVFFI7116WYMJ/), [IGVFFI9907BOPH](/sequence-files/IGVFFI9907BOPH/), [IGVFFI1994WQKG](/sequence-files/IGVFFI1994WQKG/), [IGVFFI5903FIOU](/sequence-files/IGVFFI5903FIOU/), [IGVFFI5818TRBA](/sequence-files/IGVFFI5818TRBA/), [IGVFFI8372TZIV](/sequence-files/IGVFFI8372TZIV/), [IGVFFI2138IGZI](/sequence-files/IGVFFI2138IGZI/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_missing_seqspec",
"path": "/measurement-sets/IGVFDS2584QAOI/"
}
],
"WARNING": [
{
"category": "missing auxiliary set",
"detail": "Measurement set [IGVFDS2584QAOI](/measurement-sets/IGVFDS2584QAOI/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
"level": 40,
"level_name": "WARNING",
"name": "audit_missing_auxiliary_set",
"path": "/measurement-sets/IGVFDS2584QAOI/"
}
]
},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
"title": "Jesse Engreitz"
},
"title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
},
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{
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"accession": "IGVFDS6874YIXA",
"aliases": [
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],
"status": "preview"
}
],
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"creation_timestamp": "2024-11-22T00:48:44.744041+00:00",
"donors": [
{
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"accession": "IGVFDO1080XFGV",
"aliases": [
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],
"sex": "male",
"status": "released",
"taxa": "Homo sapiens"
}
],
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},
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},
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},
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},
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},
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},
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},
{
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"treatment_term_name": "T-cell surface glycoprotein CD3 gamma chain"
}
]
}
],
"schema_version": "23",
"status": "preview",
"submitted_by": {
"@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
"title": "Michael Montgomery"
},
"submitted_files_timestamp": "2024-11-22T06:35:36.922632+00:00",
"summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
"targeted_genes": [
{
"@id": "/genes/ENSG00000134460/",
"geneid": "ENSG00000134460",
"name": "interleukin 2 receptor subunit alpha",
"status": "released",
"symbol": "IL2RA",
"synonyms": [
"interleukin-2 receptor subunit alpha",
"IL2R",
"TAC antigen",
"IMD41",
"IL-2R subunit alpha",
"CD25",
"IL-2 receptor subunit alpha",
"IDDM10",
"TCGFR",
"p55",
"interleukin 2 receptor, alpha"
]
}
],
"uuid": "3fe3ae8b-128d-4019-98d9-3861c52f433d"
}