{
"@context": "/terms/",
"@id": "/measurement-sets/IGVFDS2562AMOI/",
"@type": [
"MeasurementSet",
"FileSet",
"Item"
],
"accession": "IGVFDS2562AMOI",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep1-FFrep1-BinA_AmpliconSequencing"
],
"assay_term": {
"@id": "/assay-terms/OBI_0003661/",
"status": "released",
"term_name": "in vitro CRISPR screen using flow cytometry"
},
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Released in vitro system [IGVFSM9089XQVC](/in-vitro-systems/IGVFSM9089XQVC/) has in progress subobject construct library set [IGVFDS6377KNCH](/construct-library-sets/IGVFDS6377KNCH/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/in-vitro-systems/IGVFSM9089XQVC/"
},
{
"category": "missing sequencing kit",
"detail": "Measurement set [IGVFDS2562AMOI](/measurement-sets/IGVFDS2562AMOI/) has sequence file(s) [IGVFFI5253ZKYE](/sequence-files/IGVFFI5253ZKYE/), [IGVFFI2102XCVW](/sequence-files/IGVFFI2102XCVW/), [IGVFFI8756ZMBA](/sequence-files/IGVFFI8756ZMBA/), [IGVFFI8708RHRF](/sequence-files/IGVFFI8708RHRF/), [IGVFFI3275MZTG](/sequence-files/IGVFFI3275MZTG/), [IGVFFI1142EXLJ](/sequence-files/IGVFFI1142EXLJ/), [IGVFFI1934CGMQ](/sequence-files/IGVFFI1934CGMQ/), [IGVFFI1025DERE](/sequence-files/IGVFFI1025DERE/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_inconsistent_sequencing_kit",
"path": "/measurement-sets/IGVFDS2562AMOI/"
},
{
"category": "missing sequence specification",
"detail": "Measurement set [IGVFDS2562AMOI](/measurement-sets/IGVFDS2562AMOI/) has sequence file(s): [IGVFFI5253ZKYE](/sequence-files/IGVFFI5253ZKYE/), [IGVFFI2102XCVW](/sequence-files/IGVFFI2102XCVW/), [IGVFFI8756ZMBA](/sequence-files/IGVFFI8756ZMBA/), [IGVFFI8708RHRF](/sequence-files/IGVFFI8708RHRF/), [IGVFFI3275MZTG](/sequence-files/IGVFFI3275MZTG/), [IGVFFI1142EXLJ](/sequence-files/IGVFFI1142EXLJ/), [IGVFFI1934CGMQ](/sequence-files/IGVFFI1934CGMQ/), [IGVFFI1025DERE](/sequence-files/IGVFFI1025DERE/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_missing_seqspec",
"path": "/measurement-sets/IGVFDS2562AMOI/"
}
],
"WARNING": [
{
"category": "missing auxiliary set",
"detail": "Measurement set [IGVFDS2562AMOI](/measurement-sets/IGVFDS2562AMOI/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
"level": 40,
"level_name": "WARNING",
"name": "audit_missing_auxiliary_set",
"path": "/measurement-sets/IGVFDS2562AMOI/"
}
]
},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
"title": "Jesse Engreitz"
},
"title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
},
"control_file_sets": [
{
"@id": "/measurement-sets/IGVFDS9477ARWI/",
"accession": "IGVFDS9477ARWI",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-Jurkat-PE2_Exp1_NegControl_AmpliconSequencing",
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-Jurkat-PE2_Exp1_NegControl_AmpliconSequencing"
],
"status": "released"
}
],
"control_for": [],
"creation_timestamp": "2023-11-30T22:47:45.239841+00:00",
"donors": [
{
"@id": "/human-donors/IGVFDO1080XFGV/",
"accession": "IGVFDO1080XFGV",
"aliases": [
"igvf:donor_of_Jurkat"
],
"sex": "male",
"status": "released",
"taxa": "Homo sapiens"
}
],
"externally_hosted": false,
"file_set_type": "experimental data",
"files": [
{
"@id": "/sequence-files/IGVFFI5253ZKYE/",
"accession": "IGVFFI5253ZKYE",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep1-FFrep1-BinA-PCRrep1_I2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T19:55:50.871833+00:00",
"file_format": "fastq",
"file_size": 3224717,
"href": "/sequence-files/IGVFFI5253ZKYE/@@download/IGVFFI5253ZKYE.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/0adda537-f11d-4c09-90f9-f7d75a6aef12/IGVFFI5253ZKYE.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S12-BioRep1-FFRep1-BinA-PCRrep1_S113_I2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI2102XCVW/",
"accession": "IGVFFI2102XCVW",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep1-FFrep1-BinA-PCRrep1_R2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T19:39:04.132225+00:00",
"file_format": "fastq",
"file_size": 14149269,
"href": "/sequence-files/IGVFFI2102XCVW/@@download/IGVFFI2102XCVW.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/6c010d36-2c5a-48af-bec0-f813eda30349/IGVFFI2102XCVW.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S12-BioRep1-FFRep1-BinA-PCRrep1_S113_R2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI8756ZMBA/",
"accession": "IGVFFI8756ZMBA",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep1-FFrep1-BinA-PCRrep1_I1"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T19:47:37.214563+00:00",
"file_format": "fastq",
"file_size": 3470125,
"href": "/sequence-files/IGVFFI8756ZMBA/@@download/IGVFFI8756ZMBA.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/e45d5c76-cbe8-4ba0-a60d-28a74dbf8023/IGVFFI8756ZMBA.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S12-BioRep1-FFRep1-BinA-PCRrep1_S113_I1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI8708RHRF/",
"accession": "IGVFFI8708RHRF",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep1-FFrep1-BinA-PCRrep2_I1"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T20:20:47.953098+00:00",
"file_format": "fastq",
"file_size": 2920971,
"href": "/sequence-files/IGVFFI8708RHRF/@@download/IGVFFI8708RHRF.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/a6120e64-4a43-4872-b2d5-2c5760d6af0d/IGVFFI8708RHRF.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S12-BioRep1-FFRep1-BinA-PCRrep2_S170_I1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI3275MZTG/",
"accession": "IGVFFI3275MZTG",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep1-FFrep1-BinA-PCRrep2_I2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T20:29:04.522442+00:00",
"file_format": "fastq",
"file_size": 2864029,
"href": "/sequence-files/IGVFFI3275MZTG/@@download/IGVFFI3275MZTG.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/2711e3d2-56c0-4b42-a475-cc770af71cc6/IGVFFI3275MZTG.fastq.gz",
"sequencing_platform": {
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"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S12-BioRep1-FFRep1-BinA-PCRrep2_S170_I2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI1142EXLJ/",
"accession": "IGVFFI1142EXLJ",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep1-FFrep1-BinA-PCRrep1_R1"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T19:30:44.270902+00:00",
"file_format": "fastq",
"file_size": 7741456,
"href": "/sequence-files/IGVFFI1142EXLJ/@@download/IGVFFI1142EXLJ.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/b7f25d00-b84c-47a1-8d73-7c683011d9c8/IGVFFI1142EXLJ.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S12-BioRep1-FFRep1-BinA-PCRrep1_S113_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI1934CGMQ/",
"accession": "IGVFFI1934CGMQ",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep1-FFrep1-BinA-PCRrep2_R1"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T20:03:57.838224+00:00",
"file_format": "fastq",
"file_size": 6888103,
"href": "/sequence-files/IGVFFI1934CGMQ/@@download/IGVFFI1934CGMQ.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/5c7a27b9-4e26-43ad-9417-fa3c0e642ce5/IGVFFI1934CGMQ.fastq.gz",
"sequencing_platform": {
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"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S12-BioRep1-FFRep1-BinA-PCRrep2_S170_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI1025DERE/",
"accession": "IGVFFI1025DERE",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep1-FFrep1-BinA-PCRrep2_R2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T20:12:15.232570+00:00",
"file_format": "fastq",
"file_size": 12651012,
"href": "/sequence-files/IGVFFI1025DERE/@@download/IGVFFI1025DERE.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/f3a30511-27a7-4ad7-bb35-c3593247a7f5/IGVFFI1025DERE.fastq.gz",
"sequencing_platform": {
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"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S12-BioRep1-FFRep1-BinA-PCRrep2_S170_R2_001.fastq.gz",
"upload_status": "validated"
}
],
"input_for": [
"/analysis-sets/IGVFDS1863TJOE/"
],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set. This measurement set previously used Variant FlowFISH as a preferred_assay_title, but this enum is now removed. So it has been defaulted to Variant-EFFECTS.",
"preferred_assay_title": "Variant-EFFECTS",
"protocols": [
"https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
],
"release_timestamp": "2023-12-20T23:04:49.812287+00:00",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM9089XQVC/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM9089XQVC",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep1-FFrep1-BinA"
],
"classifications": [
"cell line"
],
"construct_library_sets": [
{
"@id": "/construct-library-sets/IGVFDS6377KNCH/",
"accession": "IGVFDS6377KNCH",
"file_set_type": "guide library",
"status": "in progress",
"summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
}
],
"modifications": [
{
"@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
"modality": "prime editing",
"status": "released"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"@type": [
"SampleTerm",
"OntologyTerm",
"Item"
],
"aliases": [
"igvf:Jurkat"
],
"status": "released",
"summary": "Jurkat",
"term_name": "Jurkat"
}
],
"status": "released",
"summary": "Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 0-10% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
"taxa": "Homo sapiens",
"treatments": [
{
"@id": "/treatments/df02c0d4-f796-4f77-bfbc-388cdbfcc615/",
"status": "released",
"treatment_term_name": "T-cell surface glycoprotein CD3 gamma chain"
},
{
"@id": "/treatments/42b3c61d-38de-4bd2-bb39-33c125a417cb/",
"status": "released",
"treatment_term_name": "phorbol 13-acetate 12-myristate"
}
]
}
],
"schema_version": "23",
"status": "released",
"submitted_by": {
"@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
"title": "Michael Montgomery"
},
"submitted_files_timestamp": "2023-12-05T19:30:44.270902+00:00",
"summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
"targeted_genes": [
{
"@id": "/genes/ENSG00000108179/",
"geneid": "ENSG00000108179",
"name": "peptidylprolyl isomerase F",
"status": "released",
"symbol": "PPIF",
"synonyms": [
"rotamase F",
"CYP3",
"cyclophilin F",
"mitochondrial cyclophilin",
"peptidyl-prolyl cis-trans isomerase, mitochondrial",
"CypD",
"Cyp-D",
"cyclophilin 3",
"cyclophilin D",
"CyP-M",
"peptidyl-prolyl cis-trans isomerase F, mitochondrial",
"PPIase F"
]
}
],
"uuid": "4c6b6af3-21c8-4e96-bc1a-079bd4073aa3"
}