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IGVFDS2504PFZO

released
File Set Type
experimental data
Summary
CRISPR prime editing Variant-EFFECTS sorted on the expression of PPIF integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Assay Term
in vitro CRISPR screen using flow cytometry
Targeted Genes
Sample Summaries
  • Homo sapiens (male) THP-1 cell line (sorting details: FACS bin for 55-65% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library
Protocols
Aliases
jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep1-FFrep1-BinD_AmpliconSequencing
Attribution
Jesse Engreitz, Stanford

Sequencing Results (Illumina)

8 items
Read Names
fastq
reads
R1
1
GAAGAAGT:TGAC
36 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
GAAGAAGT:TGAC
12 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
TCGCCTTG:TGAC
42 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
GAAGAAGT:TGAC
66 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
GAAGAAGT:TGAC
14 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
TCGCCTTG:TGAC
76 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
TCGCCTTG:TGAC
14 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
TCGCCTTG:TGAC
15 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Associated Construct Library Sets
Summary
InVitroSystem
Homo sapiens (male) THP-1 cell line (sorting details: FACS bin for 55-65% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_THP1
male
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
guide (sgRNA) library targeting sequence variants in a genomic locus

Control File Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
untransfected prime editing Variant-EFFECTS
  • jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-THP1-PE2_Exp3_NegControl_AmpliconSequencing
Jesse Engreitz, Stanford

File Sets Using This Measurement Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
prime editing Variant-EFFECTS targeting PPIF: variant effects
  • jesse-engreitz:PPIF_promoter_8mer_insertion_THP1_Variant-FlowFISH_analysis
Jesse Engreitz, Stanford