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IGVFDS2449OXNT

released
File Set Type
experimental data
Summary
CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3
Assay Term
in vitro CRISPR screen using flow cytometry
Targeted Genes
Sample Summaries
  • TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin D for 76-100% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
Construct Library Sets
  • IGVFDS8953DFJK guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3
Protocols
Aliases
jesse-engreitz:measurement_NOS3_CRISPRi_FF_R1_FFrep1_binD_PCR2_S196

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin D for 76-100% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_teloHAEC
female
Homo sapiens

Auxiliary Datasets

1 item
Accession
Type
Summary
Files
Aliases
Lab
AuxiliarySet
gRNA sequencing for CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3
  • jesse-engreitz:auxiliary_NOS3_CRISPRi_FF_R1_FFrep1_binD_PCR2_S196
Jesse Engreitz, Stanford

Documents

1 item
Page
Description
Type
Download
Preview
experimental overview
experimental protocol

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford