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IGVFDS2427TBFR

released
File Set Type
experimental data
Summary
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Assay Term
in vitro CRISPR screen using flow cytometry
Targeted Genes
Sample Summaries
  • HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
Construct Library Sets
  • IGVFDS7857SYCK Guide (sgRNA) library targeting accessible genome regions in many genomic loci
Protocols
Aliases
jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR1_Rep1_S27
jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR1_Rep2_S82
jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR2_Rep1_S33
jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR2_Rep2_S88
jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR3_Rep1_S39
jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR3_Rep2_S94
jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR4_Rep1_S45
jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR4_Rep2_S100

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford