IGVFDS2427TBFR

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

MYC

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Construct Library Sets

IGVFDS7857SYCK guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR1_Rep1_S27

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR1_Rep2_S82

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR2_Rep1_S33

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR2_Rep2_S88

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR3_Rep1_S39

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR3_Rep2_S94

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR4_Rep1_S45

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR4_Rep2_S100

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM7258NLNH

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS3139RYUI

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI1435MZPM, IGVFFI8754LKYF, IGVFFI3364QZVW, IGVFFI4506XCFK, IGVFFI2767JLED, IGVFFI9428EDUT, IGVFFI0106GQNO, IGVFFI8510KORF

jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR1_Rep1_S27
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR1_Rep2_S82
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR2_Rep1_S33
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR2_Rep2_S88
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR3_Rep1_S39
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR3_Rep2_S94
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR4_Rep1_S45
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR4_Rep2_S100

Jesse Engreitz, Stanford

IGVFDS8214HXSU

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI3328SYVP