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IGVFDS2368HJNA

released
File Set Type
experimental data
Summary
mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Assay Term
in vitro CRISPR screen using flow cytometry
Targeted Genes
Sample Summaries
  • HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
Protocols
Aliases
jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF1-BinE-PCR1-Rep1_S245
jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF1-BinE-PCR2-Rep1_S251
jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF1-BinE-PCR3-Rep1_S287
jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF1-BinE-PCR4-Rep1_S293

Sequencing Results

4 items
Read Names
fastq
reads
1
ACAGTTGA:TTGA
15 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
GACCTAAC:GCCA
21 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
ACAGTTGA:GCCA
26 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
GACCTAAC:TTGA
14 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Associated Construct Library Sets
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
guide (sgRNA) library targeting accessible genome regions in many genomic loci

Auxiliary Datasets

1 item
Accession
Type
Summary
Files
Aliases
Lab
AuxiliarySet
cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets
  • jesse-engreitz:auxiliary_sortparams_CRUDO_CCND1_Auxin6hrs-FF1
Jesse Engreitz, Stanford

Documents

1 item
Page
Description
Type
Download
Preview
overview of experimental design
experimental protocol

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford