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IGVFDS2333QYKL

released
File Set Type
experimental data
Summary
CRISPR base editing FACS screen integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide
Assay Term
in vitro CRISPR screen using flow cytometry
Sample Summaries
  • HepG2 cell line, male, Homo sapiens (sorting details: Sorted for Top 20% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)
Protocols
Aliases
richard-sherwood:18loci_uptake_Rep3_top20_ms

Sequencing Results (Illumina)

2 items
Read Names
fastq
reads
R1
1
7.3 MB
Richard Sherwood, Brigham and Women's Hospital
validated
fastq
reads
R2
1
9.8 MB
Richard Sherwood, Brigham and Women's Hospital
validated

Samples

1 item
Accession
Type
Sample Terms
Associated Construct Library Sets
Summary
InVitroSystem
HepG2 cell line, male, Homo sapiens (sorting details: Sorted for Top 20% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HepG2
male
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
guide (sgRNA) library targeting phenotype-associated variants genome-wide

File Sets Using This Measurement Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
CRISPR base editing FACS screen: guide quantifications, variant effects
  • richard-sherwood:18loci_ABE_LDLuptake_bean_analysis_set
Richard Sherwood, Brigham and Women's Hospital

Attribution

Award
HG012010
Principal Investigator(s)
Contact P.I.
Luca Pinello
Lab
Richard Sherwood, Brigham and Women's Hospital