{
"@context": "/terms/",
"@id": "/measurement-sets/IGVFDS2269HBNS/",
"@type": [
"MeasurementSet",
"FileSet",
"Item"
],
"accession": "IGVFDS2269HBNS",
"aliases": [
"jesse-engreitz:measurement_CRUDO_FAM3C_Auxin0hrs-FF1-BinF-PCR1-Rep1_S114",
"jesse-engreitz:measurement_CRUDO_FAM3C_Auxin0hrs-FF1-BinF-PCR2-Rep1_S120",
"jesse-engreitz:measurement_CRUDO_FAM3C_Auxin0hrs-FF1-BinF-PCR3-Rep1_S168",
"jesse-engreitz:measurement_CRUDO_FAM3C_Auxin0hrs-FF1-BinF-PCR4-Rep1_S174"
],
"assay_term": {
"@id": "/assay-terms/OBI_0003661/",
"assay_slims": [
"CRISPR screens"
],
"status": "released",
"term_name": "in vitro CRISPR screen using flow cytometry"
},
"audit": {
"INTERNAL_ACTION": [
{
"category": "missing publication",
"detail": "Auxiliary set [IGVFDS7833JNUC](/auxiliary-sets/IGVFDS7833JNUC/) has no `publications`. Released and archived file sets are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_file_set_missing_publication",
"path": "/auxiliary-sets/IGVFDS7833JNUC/"
},
{
"category": "missing publication",
"detail": "In vitro system [IGVFSM6177XAAS](/in-vitro-systems/IGVFSM6177XAAS/) has no `publications`. Released and archived samples are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_sample_missing_publication",
"path": "/in-vitro-systems/IGVFSM6177XAAS/"
},
{
"category": "missing publication",
"detail": "Measurement set [IGVFDS2269HBNS](/measurement-sets/IGVFDS2269HBNS/) has no `publications`. Released and archived file sets are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_file_set_missing_publication",
"path": "/measurement-sets/IGVFDS2269HBNS/"
},
{
"category": "missing sequencing kit",
"detail": "Measurement set [IGVFDS2269HBNS](/measurement-sets/IGVFDS2269HBNS/) has sequence file(s) [IGVFFI5568QDEG](/sequence-files/IGVFFI5568QDEG/), [IGVFFI4783GBNK](/sequence-files/IGVFFI4783GBNK/), [IGVFFI8149ZFKP](/sequence-files/IGVFFI8149ZFKP/), [IGVFFI2576YRDI](/sequence-files/IGVFFI2576YRDI/) which lack specification of a `sequencing_kit`. Sequence files in a file set associated wtih bulk data should specify a sequencing kit.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_inconsistent_sequencing_kit",
"path": "/measurement-sets/IGVFDS2269HBNS/"
}
],
"WARNING": [
{
"category": "missing nucleic acid delivery",
"detail": "In vitro system [IGVFSM6177XAAS](/in-vitro-systems/IGVFSM6177XAAS/) has `construct_library_sets` but is missing `nucleic_acid_delivery`. Samples linked to construct library sets are expected to specify nucleic acid delivery method.",
"level": 40,
"level_name": "WARNING",
"name": "audit_missing_nucleic_acid_delivery",
"path": "/in-vitro-systems/IGVFSM6177XAAS/"
}
]
},
"auxiliary_sets": [
{
"@id": "/auxiliary-sets/IGVFDS7833JNUC/",
"accession": "IGVFDS7833JNUC",
"aliases": [
"jesse-engreitz:auxiliary_sortparams_CRUDO_FAM3C_Auxin0hrs-FF1"
],
"file_set_type": "cell sorting",
"status": "released"
}
],
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
"title": "Jesse Engreitz"
},
"title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
},
"construct_library_sets": [
{
"@id": "/construct-library-sets/IGVFDS1891GTLU/",
"accession": "IGVFDS1891GTLU",
"file_set_type": "guide library",
"integrated_content_files": [
{
"@id": "/tabular-files/IGVFFI0842RREU/",
"accession": "IGVFFI0842RREU",
"content_type": "guide RNA sequences",
"status": "released",
"summary": "guide RNA sequences"
}
],
"status": "released",
"summary": "guide (sgRNA) library targeting accessible genome regions in many genomic loci"
}
],
"control_for": [],
"creation_timestamp": "2024-05-14T16:16:19.018273+00:00",
"documents": [
"/documents/74d53b9c-ab1d-42e3-b679-d3a00ae3d842/"
],
"donors": [
{
"@id": "/human-donors/IGVFDO0384OZRW/",
"accession": "IGVFDO0384OZRW",
"aliases": [
"igvf:donor_of_HCT116"
],
"sex": "male",
"status": "released",
"taxa": "Homo sapiens"
}
],
"externally_hosted": false,
"file_set_type": "experimental data",
"files": [
{
"@id": "/configuration-files/IGVFFI0298DADK/",
"accession": "IGVFFI0298DADK",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_FAM3C_Auxin0hrs-FF1-BinF-PCR4-Rep1_S174"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:42:44.189975+00:00",
"file_format": "yaml",
"file_size": 913,
"href": "/configuration-files/IGVFFI0298DADK/@@download/IGVFFI0298DADK.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/573e5b92-ba48-4dd9-a4f6-84b03d062009/IGVFFI0298DADK.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.FAM3C_Auxin0hrs-FF1-BinF-PCR4-Rep1_S174.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/configuration-files/IGVFFI3382VPDH/",
"accession": "IGVFFI3382VPDH",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_FAM3C_Auxin0hrs-FF1-BinF-PCR3-Rep1_S168"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:42:30.866460+00:00",
"file_format": "yaml",
"file_size": 913,
"href": "/configuration-files/IGVFFI3382VPDH/@@download/IGVFFI3382VPDH.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/d0731797-1c2c-42d3-befd-781eb9537c4e/IGVFFI3382VPDH.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.FAM3C_Auxin0hrs-FF1-BinF-PCR3-Rep1_S168.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/configuration-files/IGVFFI6386TNSY/",
"accession": "IGVFFI6386TNSY",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_FAM3C_Auxin0hrs-FF1-BinF-PCR2-Rep1_S120"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:42:17.732548+00:00",
"file_format": "yaml",
"file_size": 915,
"href": "/configuration-files/IGVFFI6386TNSY/@@download/IGVFFI6386TNSY.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/073fe174-23a9-4ed8-94c6-a5374dfb67fe/IGVFFI6386TNSY.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.FAM3C_Auxin0hrs-FF1-BinF-PCR2-Rep1_S120.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/configuration-files/IGVFFI0978YZEF/",
"accession": "IGVFFI0978YZEF",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_FAM3C_Auxin0hrs-FF1-BinF-PCR1-Rep1_S114"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:42:02.503181+00:00",
"file_format": "yaml",
"file_size": 914,
"href": "/configuration-files/IGVFFI0978YZEF/@@download/IGVFFI0978YZEF.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/fb7e525e-adb8-4744-b3cf-3de198e5983b/IGVFFI0978YZEF.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.FAM3C_Auxin0hrs-FF1-BinF-PCR1-Rep1_S114.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/sequence-files/IGVFFI5568QDEG/",
"accession": "IGVFFI5568QDEG",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_FAM3C_Auxin0hrs-FF1-BinF-PCR4-Rep1_S174"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-01T01:52:03.866451+00:00",
"file_format": "fastq",
"file_size": 35909910,
"href": "/sequence-files/IGVFFI5568QDEG/@@download/IGVFFI5568QDEG.fastq.gz",
"s3_uri": "s3://igvf-files/2024/06/01/dca53366-dbca-4db0-ac2e-7cec9b4638a9/IGVFFI5568QDEG.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008565/",
"status": "released",
"term_name": "Illumina HiSeq 2500"
},
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/FAM3C-Auxin0hrs-FF1-BinF-PCR4-Rep1_S174_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI4783GBNK/",
"accession": "IGVFFI4783GBNK",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_FAM3C_Auxin0hrs-FF1-BinF-PCR3-Rep1_S168"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-01T01:51:44.617302+00:00",
"file_format": "fastq",
"file_size": 32360808,
"href": "/sequence-files/IGVFFI4783GBNK/@@download/IGVFFI4783GBNK.fastq.gz",
"s3_uri": "s3://igvf-files/2024/06/01/7b035173-ed36-4ade-b66d-ae68ac6b6809/IGVFFI4783GBNK.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008565/",
"status": "released",
"term_name": "Illumina HiSeq 2500"
},
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/FAM3C-Auxin0hrs-FF1-BinF-PCR3-Rep1_S168_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI8149ZFKP/",
"accession": "IGVFFI8149ZFKP",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_FAM3C_Auxin0hrs-FF1-BinF-PCR2-Rep1_S120"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-01T01:51:25.640751+00:00",
"file_format": "fastq",
"file_size": 24747787,
"href": "/sequence-files/IGVFFI8149ZFKP/@@download/IGVFFI8149ZFKP.fastq.gz",
"s3_uri": "s3://igvf-files/2024/06/01/bda80f1e-61c9-48c1-a4e7-66e9352bae5b/IGVFFI8149ZFKP.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008565/",
"status": "released",
"term_name": "Illumina HiSeq 2500"
},
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/FAM3C-Auxin0hrs-FF1-BinF-PCR2-Rep1_S120_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI2576YRDI/",
"accession": "IGVFFI2576YRDI",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_FAM3C_Auxin0hrs-FF1-BinF-PCR1-Rep1_S114"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-01T01:51:07.165063+00:00",
"file_format": "fastq",
"file_size": 22272483,
"href": "/sequence-files/IGVFFI2576YRDI/@@download/IGVFFI2576YRDI.fastq.gz",
"s3_uri": "s3://igvf-files/2024/06/01/8f3c1113-a1db-41bd-b7a5-f56c55a8ce79/IGVFFI2576YRDI.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008565/",
"status": "released",
"term_name": "Illumina HiSeq 2500"
},
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/FAM3C-Auxin0hrs-FF1-BinF-PCR1-Rep1_S114_R1_001.fastq.gz",
"upload_status": "validated"
}
],
"input_for": [
"/analysis-sets/IGVFDS5239VQTN/"
],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set.",
"preferred_assay_title": "CRISPR FlowFISH screen",
"protocols": [
"https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1"
],
"release_timestamp": "2024-07-10T19:40:17.231305+00:00",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM6177XAAS/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM6177XAAS",
"aliases": [
"jesse-engreitz:in-vitro-system_CRUDO_FAM3C_Auxin0hrs-FF1-BinF-PCR1-Rep1_S114",
"jesse-engreitz:in-vitro-system_CRUDO_FAM3C_Auxin0hrs-FF1-BinF-PCR2-Rep1_S120",
"jesse-engreitz:in-vitro-system_CRUDO_FAM3C_Auxin0hrs-FF1-BinF-PCR3-Rep1_S168",
"jesse-engreitz:in-vitro-system_CRUDO_FAM3C_Auxin0hrs-FF1-BinF-PCR4-Rep1_S174"
],
"classifications": [
"cell line"
],
"institutional_certificates": [],
"modifications": [
{
"@id": "/crispr-modifications/8543fa06-1720-4e7d-8e8f-7e24c1b1cf79/",
"modality": "interference",
"status": "released"
},
{
"@id": "/degron-modifications/2e8d10cc-fc98-44d5-a7d9-e2892e16c98a/",
"modality": "degradation",
"status": "released"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002824/",
"@type": [
"SampleTerm",
"OntologyTerm",
"Item"
],
"aliases": [
"igvf:HCT116"
],
"status": "released",
"summary": "HCT116",
"term_name": "HCT116"
}
],
"status": "released",
"summary": "Homo sapiens (male) HCT116 cell line (sorting details: FACS bin F for 80-100% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library",
"taxa": "Homo sapiens"
}
],
"schema_version": "26",
"status": "released",
"submitted_by": {
"@id": "/users/3bd6f74c-8cf3-4c35-a94d-0c7c11654815/",
"title": "Chongyon Park"
},
"submitted_files_timestamp": "2024-06-01T01:51:07.165063+00:00",
"summary": "mixed CRISPR FlowFISH screen sorted on the expression of FAM3C integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci",
"targeted_genes": [
{
"@id": "/genes/ENSG00000196937/",
"geneid": "ENSG00000196937",
"name": "FAM3 metabolism regulating signaling molecule C",
"status": "released",
"symbol": "FAM3C",
"synonyms": [
"FAM3 metabolism regulating signaling molecule C",
"FAM3C",
"interleukin-like EMT inducer",
"protein FAM3C",
"predicted osteoblast protein",
"family with sequence similarity 3 member C",
"ILEI",
"GS3786",
"interleukin-like epithelial-mesenchymal transition inducer"
]
}
],
"uuid": "109dedc8-58ec-4267-9b56-0cb9dc4906a6"
}