IGVFDS2241NBBY

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

CCND1

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Construct Library Sets

IGVFDS6351FGNG guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_CCND1_Auxin0hrs-FF1-BinF-PCR1-Rep1_S222

jesse-engreitz:measurement_CRUDO_CCND1_Auxin0hrs-FF1-BinF-PCR2-Rep1_S228

jesse-engreitz:measurement_CRUDO_CCND1_Auxin0hrs-FF1-BinF-PCR3-Rep1_S264

jesse-engreitz:measurement_CRUDO_CCND1_Auxin0hrs-FF1-BinF-PCR4-Rep1_S270

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM4218VHTT

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS4259ETEA

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI9279HEMN, IGVFFI6818RKHX, IGVFFI8918ZHNH, IGVFFI5127EQNC

jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin0hrs-FF1-BinF-PCR1-Rep1_S222
jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin0hrs-FF1-BinF-PCR2-Rep1_S228
jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin0hrs-FF1-BinF-PCR3-Rep1_S264
jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin0hrs-FF1-BinF-PCR4-Rep1_S270

Jesse Engreitz, Stanford

IGVFDS5464XLTY

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI0860RTJY