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IGVFDS2226SPYF

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File Set Type
experimental data
Summary
CRISPR prime editing Variant-EFFECTS sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Assay Term
in vitro CRISPR screen using flow cytometry
Targeted Genes
Sample Summaries
  • Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 76-100% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library
Protocols
Aliases
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep3-BinD_AmpliconSequencing
Attribution
Jesse Engreitz, Stanford

Sequencing Results (Illumina)

32 items
Read Names
fastq
reads
I2
1
AGGTCGCA:TTGT
1.4 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
GCAGCCGT:TTGT
1.6 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
AGGTGCGA:TTGT
12 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
GCAGCCGT:TTGT
6.1 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
AGGTGCGA:GGCA
8.6 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
AGGTGCGA:GGCA
1.8 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
AGGTGCGA:GGCA
6.5 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
GCCGCAAC:GGCA
9.0 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
AGGTCGCA:TTGT
5.9 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
GCCGCAAC:GGCA
1.9 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Associated Construct Library Sets
Summary
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 76-100% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_Jurkat
male
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
guide (sgRNA) library targeting sequence variants in a genomic locus

Control File Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
prime editing Variant-EFFECTS sorted on the expression of IL2RA
  • jesse-engreitz:Jurkat_Stim-IL2RA_prom-NegControl_AmpliconSequencing
Jesse Engreitz, Stanford

File Sets Using This Measurement Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
prime editing Variant-EFFECTS targeting IL2RA: variant effects
  • jesse-engreitz:IL2RA_promoter_tiling_Jurkat-Stimulation_Variant-EFFECTS_analysis
Jesse Engreitz, Stanford