IGVFDS2171ROQP

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

FAM3C

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Construct Library Sets

IGVFDS1891GTLU guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_FAM3C_Auxin6hrs-FF1-BinB-PCR1-Rep1_S134

jesse-engreitz:measurement_CRUDO_FAM3C_Auxin6hrs-FF1-BinB-PCR2-Rep1_S140

jesse-engreitz:measurement_CRUDO_FAM3C_Auxin6hrs-FF1-BinB-PCR3-Rep1_S188

jesse-engreitz:measurement_CRUDO_FAM3C_Auxin6hrs-FF1-BinB-PCR4-Rep1_S194

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9056QVLB

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS6385IAUC

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI8034QLTO, IGVFFI8323VZPJ, IGVFFI6260ZECC, IGVFFI6399FWUL

jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin6hrs-FF1-BinB-PCR1-Rep1_S134
jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin6hrs-FF1-BinB-PCR2-Rep1_S140
jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin6hrs-FF1-BinB-PCR3-Rep1_S188
jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin6hrs-FF1-BinB-PCR4-Rep1_S194

Jesse Engreitz, Stanford

IGVFDS9190KTAF

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI8850HGLT